Information on EC 3.4.21.119 - kallikrein 13

Word Map on EC 3.4.21.119
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.119
-
RECOMMENDED NAME
GeneOntology No.
kallikrein 13
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolyses mouse Ren2 protein (a species of prorenin present in the submandibular gland) on the carboxy side of the arginine residue at the Lys-Arg-/- pair in the N-terminus, to yield mature renin
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
342900-44-1
-
9001-01-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme belongs to the human kallikrein-related peptidase family, which is comprised of 15 serine protease genes on chromosome 19q13.4
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
35 kDa pro-interleukin 1beta + H2O
17.5 kDa interleukin 1beta + 20 kDa interleukin 1beta
show the reaction diagram
-
cleavage of Leu113-Leu114 bond
-
-
?
Abz-GFSPFRSSRIQ-EDDnp + H2O
Abz-GFSPFR + SSRIQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLKSSKQ-EDDnp + H2O
Abz-KLK + SSKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLRKSKQ-EDDnp + H2O
Abz-KLR + KSKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLRRSKQ-EDDnp + H2O
Abz-KLR + RSKQ-EDDnp
show the reaction diagram
-
-
-
-
?
Abz-KLRSSKQ-EDDnp + H2O
Abz-KLR + SSKQ-EDDnp
show the reaction diagram
-
best substrate
-
-
?
Abz-MISLMKRPPGFSPFRSSRI-NH2 + H2O
Abz-MISLMKRPPGFSPFR + SSRI-NH2
show the reaction diagram
-
rapid cleavage
-
-
?
Abz-MISLMKRPQ-EDDnp + H2O
Abz-MISLMK + RPQ-EDDnp
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Arg-Val-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Arg-Val-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity
-
-
?
benzyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Gln-Arg-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
best substrate
-
-
?
benzyloxycarbonyl-Glu-Lys-Lys-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Glu-Lys-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity
-
-
?
benzyloxycarbonyl-Leu-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
benzyloxycarbonyl-Leu-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Boc-Phe-Ser-Arg-7-amido-4-methylcoumarin + H2O
?
show the reaction diagram
-
-
-
-
?
FSR-amido-4-methylcoumarin + H2O
FSR + 7-amino-4-methylcoumarin
show the reaction diagram
-
fluorogenic trypsin-like peptide substrate
-
-
?
histatin 3 + H2O
DSHAKRHHGYKRKFHEKHHSHRGYR + SNYLYDN
show the reaction diagram
-
i.e. DSHAKRHHGYKRKFHEKHHSHRGYRSNYLYDN, efficient substrate
-
-
?
low molecular weight human kininogen + H2O
?
show the reaction diagram
-
-
-
-
?
mouse prorenin Ren2 + H2O
renin + ?
show the reaction diagram
-
specific cleavage of Lys-Arg bond of mouse prorenin Ren2
-
-
?
myelin basic protein + H2O
?
show the reaction diagram
-
at least two cleavage sites are identified at YGGR-ASDYK and PRHR-DTGI
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
Prorenin + H2O
Renin + ?
show the reaction diagram
pyroglutamyl-Arg-Tyr-Lys-Arg-4-methyl-coumaryl-7-amide + H2O
pyroglutamyl-Arg-Tyr-Lys-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
VPR-amido-4-methylcoumarin + H2O
VPR + 7-amino-4-methylcoumarin
show the reaction diagram
-
fluorogenic trypsin-like peptide substrate
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polypeptide + H2O
peptides
show the reaction diagram
Prorenin + H2O
Renin + ?
show the reaction diagram
additional information
?
-
-
enzyme is involved in tissue remodeling and/or tumor invasion and metastasis
-
-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
Na+, K+, Mg2+ and Ca2+ up to 1 M concentration do not activate the enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
antipain
benzamidine
diisopropyl fluorophosphate
-
2 mM, 35% inhibition
leupeptin
Phenylmethylsulfonylfluoride
-
-
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Chondroitin
-
-
dermatan sulfate
-
-
enterokinase
-
-
-
Sodium citrate
-
-
Sodium sulfate
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0053 - 0.031
Abz-KLKSSKQ-EDDnp
0.0012 - 0.0022
Abz-KLRSSKQ-EDDnp
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1 - 0.5
Abz-KLKSSKQ-EDDnp
3.2 - 3.4
Abz-KLRSSKQ-EDDnp
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
16 - 19
Abz-KLKSSKQ-EDDnp
19572
1455 - 2834
Abz-KLRSSKQ-EDDnp
10357
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0011
-
purified enzyme, substrate benzyloxycarbonyl-Gln-Arg-Arg-4-methyl-coumaryl-7-amide
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
assay at
7.5 - 8.5
-
-
7.5
-
assay at
8 - 8.5
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.5 - 9.8
-
-
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
from women with advanced ovarian carcinoma
Manually annotated by BRENDA team
down-regulated by steroid hormones
Manually annotated by BRENDA team
-
expression of the KLK13 and KLK14 genes are examined at the mRNA and protein levels in a cohort of 57 patients with non-small-cell lung cancer. The mRNA levels, assessed by real-time RT-PCR, are significantly different in malignant tissues compared to adjacent non-malignant tissues. KLK13 and KLK14 mRNA overexpression in tumors is associated with a positive nodal status in multivariate analysis
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
kallikrein-related peptidase 13 is downregulated in oral squamous cell carcinoma cell lines, primary tumors and established cell lines
Manually annotated by BRENDA team
-
modest concentration
Manually annotated by BRENDA team
-
the clinical value of 11 members of the tissue kallikrein family as potential biomarkers for lung cancer diagnosis are analyzed: Serum specimens from 51 patients with non-small cell lung cancer and from50 healthy volunteers are collected. Samples are analyzed for 11 kallikreins (KLK1, KLK4-8, and KLK10-14) by specific ELISA. compared with sera from normal subjects, sera of patients with non-small cell lung cancer have lower levels of KLK5, KLK7, KLK8, KLK10, and KLK12, and higher levels of KLK11, KLK13, and KLK14. KLK11, KLK12, KLK13, and KLK14 are associated with higher risk of non-small cell lung cancer as determined by univariate analysis and confirmed by multivariate analysis
Manually annotated by BRENDA team
-
hK13 is present at significantly lower concentrations in females than in males; quantification of eight different tissue kallikreins. Enzyme is present at significantly lower concentrations in females than in males
Manually annotated by BRENDA team
-
from the face with highest amounts, in the eccrine sweat glands regardless of gender; quantification of eight different tissue kallikreins. Enzyme is detected only in the eccrine sweat glands regardless of gender
Manually annotated by BRENDA team
-
low concentration
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
of granular convoluted tubular cell, co-localization of enzyme and pro-interleukin 1beta
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
-
1 * 17000 + 1 * 10000, SDS-PAGE
17000
-
1 * 17000 + 1 * 10000, SDS-PAGE
30000
-
secreted enzyme, gel filtration
50000
-
x * 50000, recombinant glycosylated enzyme, SDS-PAGE
54000
-
about, gel filtration and crystal structure
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
both hanging and sitting drop vapour diffusion method, protein solution: 57 mg/ml, precipitant solution: 10-15% PEG 8000, 200 mM Li2SO4, 100 mM sodium cacodylate, pH 6.5, room temperature, X-ray diffraction structure determination and analysis
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
KLK13 activity is reduced close to zero in the presence of 50 mM Tris HCl (with no sodium citrate or heparin)
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
-
recombinant enzyme from Pichia pastoris
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in a baculovirus/insect cell line system
-
expression in Pichia pastoris
-
gene KLK-L4, DNA sequence determination and analysis, gene mapps to chromosome 19q13.3-q13.4, tissue expression analysis
gene KLK13, encoded on chromosome 19q13.4, quantitative real-time PCR expression analysis
-
gene KLK13, quantitative real-time PCR expression anaysis
-
the enzyme is encoded on chromosome 19q13.4
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
kallikrein-related peptidase 13 is downregulated in oral squamous cell carcinoma cell lines
the kallikrein-related peptidase 13 gene is substantially up-regulated after exposure of gastric cancer cells to antineoplastic agents, epirubicin, oxaliplatin, or methotrexate
-
the KLK13 gene is dysregulated in several carcinomas, and its expression levels seem to be associated with disease prognosis
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
treatment of ovarian cancer cell lines secreting enzyme with an enzyme neutralizing antibody results in reduced migration of cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
medicine