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Information on EC 3.4.21.118 - kallikrein 8 and Organism(s) Rattus norvegicus and UniProt Accession O88780

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.118 kallikrein 8
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This record set is specific for:
Rattus norvegicus
UNIPROT: O88780 not found.
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys
Synonyms
neuropsin, kallikrein-related peptidase, kallikrein-8, kallikrein 8, tadg-14, kallikrein-related peptidase 8, tadg14, rgk-8, type ii neuropsin, brain serine protease 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
brain serine protease 1
-
kallikrein-8
-
-
-
-
neuropsin
-
-
-
-
P1 kallikrein
-
S01.244
-
-
-
-
tissue kallikrein
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cleavage of amide substrates following the basic amino acids Arg or Lys at the P1 position, with a preference for Arg over Lys
show the reaction diagram
kallikrein k8 exhibit a marked preference for cleavage downstream of Arg residues
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
171715-15-4
-
9001-01-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
benzoyl-Phe-Val-Arg-4-methylcoumarin 7-amide + H2O
benzoyl-Phe-Val-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
benzyloxycarbonyl-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
polypeptide + H2O
peptides
show the reaction diagram
-
-
-
?
Pro-Phe-Arg-4-methylcoumaryl-7-amide
Pro-Phe-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
tert-butyloxycarbonyl-Phe-Ser-Arg-4-methylcoumaryl-7-amide + H2O
tert-butyloxycarbonyl-Phe-Ser-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
no activity
-
-
?
tert-butyloxycarbonyl-Val-Pro-Arg-4-methylcoumaryl-7-amide + H2O
tert-butyloxycarbonyl-Val-Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polypeptide + H2O
peptides
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
no inhibition by soybean trypsin inhibitor
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
primary cardiomyocyte
Manually annotated by BRENDA team
KLK8 expression is upregulated in left ventricle of cardiac hypertrophy models
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
both intra-cardiac adenovirus-mediated and transgenic-mediated KLK8 overexpression leads to cardiac hypertrophy in vivo. In primary neonatal rat cardiomyocytes, KLK8 knockdown inhibits phenylephrine-induced cardiomyocyte hypertrophy, whereas KLK8 overexpression promotes cardiomyocyte hypertrophy via a serine protease activity-dependent but kinin receptor-independent pathway. KLK8 overexpression increases epidermal growth factor production, which is blocked by the inhibitors of serine protease. EGF receptor antagonist and EGFR knockdown reverse the hypertrophy induced by KLK8 overexpression. KLK8-induced cardiomyocyte hypertrophy is also significantly decreased by blocking the protease-activated receptor PAR1 or PAR2 pathway
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
KLK8_RAT
260
1
28510
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
-
x * 10000 + x * 18000, SDS-PAGE
18000
-
x * 10000 + x * 18000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 10000 + x * 18000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Boeckmann, B.; Bairoch, A.; Apweiler, R.; Blatter, M.C.; Estreicher, A.; Gasteiger, E.; Martin M.J.; Michoud, K.; O'Donovan, C.; Phan, I.; Pilbout, S.; Schneider, M.
The SWISS-PROT protein knowledgebase and its supplement TrEMBL
Nucleic Acids Res.
31
365-370
2003
Homo sapiens (O60259), Mus musculus (P07628), Mus musculus (Q61955), Rattus norvegicus (O88780), Rattus norvegicus (P36374)
Manually annotated by BRENDA team
El Moujahed, A.; Gutman, N.; Brillard, M.; Gauthier, F.
Substrate specificity of two kallikrein family gene products isolated from the rat submandibular gland
FEBS Lett.
265
137-140
1990
Rattus norvegicus
Manually annotated by BRENDA team
Cao, B.; Yu, Q.; Zhao, W.; Tang, Z.; Cong, B.; Du, J.; Lu, J.; Zhu, X.; Ni, X.
Kallikrein-related peptidase 8 is expressed in myocardium and induces cardiac hypertrophy
Sci. Rep.
7
20024
2016
Rattus norvegicus (O88780)
Manually annotated by BRENDA team