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Information on EC 3.4.21.108 - HtrA2 peptidase and Organism(s) Drosophila melanogaster and UniProt Accession Q9VFJ3

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.108 HtrA2 peptidase
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This record set is specific for:
Drosophila melanogaster
UNIPROT: Q9VFJ3 not found.
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Word Map
The taxonomic range for the selected organisms is: Drosophila melanogaster
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
cleavage of non-polar aliphatic amino-acids at the P1 position, with a preference for Val, Ile and Met. At the P2 and P3 positions, Arg is selected most strongly with a secondary preference for other hydrophilic residues
Synonyms
omi/htra2, htra2/omi, serine protease omi/htra2, serine protease htra2/omi, serine protease htra2, high temperature requirement a2, htra2 protease, omi/htra2 protease, omi protease, high temperature requirement protein a2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dOmi/HtrA2
two isoforms, dOmi-L, dOmi-S
high temperature requirement A2
-
Omi/HtrA2 serine protease
-
HtrA2 protease
-
-
-
-
serine protease HtrA2/Omi
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
CAS REGISTRY NUMBER
COMMENTARY hide
204655-80-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
cleavage of DIAP1 between the BIR1 and BIR2 domain and further degradation of BIR1
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
cleavage of DIAP1 between the BIR1 and BIR2 domain and further degradation of BIR1
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
in Omi/HtrA2 silenced Drosophila S2R+ cells elongated mitochondria are found by live cell imaging. Electron microscopy confirm the mitochondrial morphology alterations and show abnormal cristae structure. Examining the levels of proteins involved in mitochondrial fusion, a selective up-regulation of optic atrophy protein 1 is shown
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HTRA2_DROME
422
0
46000
Swiss-Prot
Mitochondrion (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
dOmi-S, determined by SDS-PAGE and Western Blot analysis
35000
37000
cleaved form, mitochondrial import
38000
unprocessed dOmi, determined by SDS-PAGE and Western Blot analysis
46000
full-length protein
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
G363S
Drosophila Omi mutant analogous to human Omi/HtrA2 mutation G399S
HtrA2DELTA1
deletion mutant, the P-element G4907 is excised to produce a deletion of 1037 bp
S236C
Drosophila Omi mutant analogous to human Omi/HtrA2 mutation S276C
S266A
Drosophila Omi mutant analogous to human Omi/HtrA2 mutation S306A
S364A
Drosophila Omi mutant analogous to human Omi/HtrA2 mutation S400A
S306A
-
mutant shows abolished protease activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using TALON agarose
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
constructs encoding C-terminal His6-tagged full-length dOmi or truncated mutants are generated using the pET-21a and the pVL-1393 transfer vector, proteins are overexpressed in Escherichia coli BL21DE3 and Sf9 insect cells, respectively
into the vectors pCaSpeR-HS and pUASp
into the vectors pUASt and pTMR
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Vande Walle, L.; Lamkanfi, M.; Vandenabeele, P.
The mitochondrial serine protease HtrA2/Omi: an overview
Cell Death Differ.
15
453-460
2008
Bacillus sp. (in: Bacteria), Bos taurus, Canis lupus familiaris, Clostridium sp., Drosophila melanogaster, Escherichia coli, Homo sapiens, Macaca mulatta, Mesorhizobium loti, Mus musculus, no activity in Caenorhabditis elegans, no activity in Mycoplasma genitalium, no activity in Mycoplasma pneumoniae, Rattus norvegicus
Manually annotated by BRENDA team
Khan, F.S.; Fujioka, M.; Datta, P.; Fernandes-Alnemri, T.; Jaynes, J.B.; Alnemri, E.S.
The interaction of DIAP1 with dOmi/HtrA2 regulates cell death in Drosophila
Cell Death Differ.
15
1073-1083
2008
Drosophila melanogaster (Q9VFJ3)
Manually annotated by BRENDA team
Tain, L.S.; Chowdhury, R.B.; Tao, R.N.; Plun-Favreau, H.; Moisoi, N.; Martins, L.M.; Downward, J.; Whitworth, A.J.; Tapon, N.
Drosophila HtrA2 is dispensable for apoptosis but acts downstream of PINK1 independently from Parkin
Cell Death Differ.
16
1118-1125
2009
Drosophila melanogaster (Q9VFJ3)
Manually annotated by BRENDA team
Yun, J.; Cao, J.H.; Dodson, M.W.; Clark, I.E.; Kapahi, P.; Chowdhury, R.B.; Guo, M.
Loss-of-function analysis suggests that Omi/HtrA2 is not an essential component of the PINK1/PARKIN pathway in vivo
J. Neurosci.
28
14500-14510
2008
Drosophila melanogaster (Q9VFJ3)
Manually annotated by BRENDA team
Kieper, N.; Holmstroem, K.M.; Ciceri, D.; Fiesel, F.C.; Wolburg, H.; Ziviani, E.; Whitworth, A.J.; Martins, L.M.; Kahle, P.J.; Krueger, R.
Modulation of mitochondrial function and morphology by interaction of Omi/HtrA2 with the mitochondrial fusion factor OPA1
Exp. Cell Res.
316
1213-1224
2010
Drosophila melanogaster, Homo sapiens, Mus musculus
Manually annotated by BRENDA team