Information on EC 3.4.21.101 - xanthomonalisin

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.21.101
-
RECOMMENDED NAME
GeneOntology No.
xanthomonalisin
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cleavage of casein
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
serine endopeptidase
-
CAS REGISTRY NUMBER
COMMENTARY hide
113356-29-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain No. 101
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Acid-denatured hemoglobin + H2O
?
show the reaction diagram
casein + H2O
?
show the reaction diagram
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ala-Asp-Phe + (4-nitro)Phe-Arg-Leu
show the reaction diagram
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ala-Leu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu + H2O
Lys-Pro-Ala-Lys-Phe + (4-nitro)Phe-Arg-Leu
show the reaction diagram
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Arg-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asn-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Asp-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Gln-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Glu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ala-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Arg-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asn-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Asp-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Gln-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ala-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ala
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Arg
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Asp
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ile
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Phe
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Ser
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Tyr
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Arg-Val
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asn-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Asp-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Glu-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ile-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Leu-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Lys-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Ser-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu + H2O
Lys-Pro-Ile-Glu-Phe + p-nitrophenylalanine-Val-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-His-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ile-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Leu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Lys-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Nle-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Pro-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Ser-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Thr-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ile-Val-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Leu-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Lys-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Phe-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Ser-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Thr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Tyr-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu + H2O
Lys-Pro-Val-Glu-Phe + p-nitrophenylalanine-Arg-Leu
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
Ca2+-binding site
additional information
-
no inhibition or activation by divalent cations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-epoxy-3-(4-nitrophenoxy)propane
chymostatin
-
weak
HgCl2
-
slight
isovaleryl-tyrosyl-leucyl-tyrosinal
tyrostatin
Microbial alkaline protease inhibitor alpha
-
weak
-
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0188 - 0.0191
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu
0.00605
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0058 - 0.0188
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
0.0823 - 0.0829
Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu
0.00932
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0115
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0139
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.013
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.012
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.005
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0135
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00531
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0026
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0126
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0174
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
-
pH 3.5, 37°C
0.00763
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
-
pH 3.5, 37°C
0.0063
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
-
pH 3.5, 37°C
0.00995
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
-
pH 3.5, 37°C
0.00824
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
-
pH 3.5, 37°C
0.0106
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00746
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
-
pH 3.5, 37°C
0.00982
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
-
pH 3.5, 37°C
0.00944
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
-
pH 3.5, 37°C
0.00532
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
-
pH 3.5, 37°C
0.0183
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
-
pH 3.5, 37°C
0.0213
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
-
pH 3.5, 37°C
0.0217
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
-
pH 3.5, 37°C
0.00977
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
-
pH 3.5, 37°C
0.0283
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
-
pH 3.5, 37°C
0.0158
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
-
pH 3.5, 37°C
0.0164
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
-
pH 3.5, 37°C
0.0138
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
-
pH 3.5, 37°C
0.0101
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00716
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00214
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0173
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0662
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00782
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00523
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00451
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00445
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0114
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0183
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0105
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0039
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.0071
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00224
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
0.00995
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
-
pH 3.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20.4
Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu
Pseudomonas sp.
-
autentic and recombinant PCP
0.037 - 79.4
Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu
0.3 - 68.4
Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu
19.2
Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu
Pseudomonas sp.
-
autentic and recombinant PCP
105
Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
38.3
Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
18.1
Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
2.2 - 73.9
Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu
34 - 62.4
Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
60.5
Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
43.3
Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
0.8 - 35.7
Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu
18.6
Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
48.8
Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
1.4
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
24.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala
Xanthomonas sp.
-
pH 3.5, 37°C
15.5
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg
Xanthomonas sp.
-
pH 3.5, 37°C
101
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp
Xanthomonas sp.
-
pH 3.5, 37°C
27.3
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile
Xanthomonas sp.
-
pH 3.5, 37°C
102
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
26.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe
Xanthomonas sp.
-
pH 3.5, 37°C
1.6 - 71.3
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser
27.6
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr
Xanthomonas sp.
-
pH 3.5, 37°C
30.9
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val
Xanthomonas sp.
-
pH 3.5, 37°C
32.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
20 - 87.9
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu
189
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
16.1
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
31
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
43.7
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
40.4
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
0.04 - 8.57
Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu
16.9 - 23.1
Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu
1.1 - 19.4
Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu
16.8
Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
0.97 - 16.6
Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu
39.8
Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
6 - 57.9
Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu
56.8
Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
30.5
Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
15.9 - 26.9
Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu
15.3
Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
96.4
Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
2.6 - 57.3
Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu
3.4 - 36.7
Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu
37
Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
8.45
Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
28.1
Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu
Xanthomonas sp.
-
pH 3.5, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000021
isovaleryl-tyrosyl-leucyl-tyrosinal
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
acid-denatured hemoglobin, casein
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
gel filtration
42000
-
gel filtration
43000
-
1 * 43000, SDS-PAGE
61070
-
calculation from PCP gene, processed and secreted into the periplasm as a 43000 protein, which, under acidic conditions converts to mature PCP
84000
recombinant protein from Escherichia coli
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
N-terminal propeptide and C-terminal propeptide
additional information
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 5.3
-
37°C, 20 h, stable
137235
4 - 5.5
-
37°C, 15 h, stable
137232
4.8
-
15 min, stable below 50°C, complete loss of activity at 60°C
137232
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
15 min, stable below
50
-
pH 4.8, 15 min, stable below
60
-
pH 4.8, 15 min, complete loss of activity
65
-
15 min, complete loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant mutant enzymes S79A, S169A and D348A
-
strain No. 101
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D348A
-
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
DELTA192
-
pro-region mutant enzyme
S169A
-
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected
S79A
-
production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected