the best substrate of the type Lys-Pro-Ile(P3)-Glu(P2)-Phe-/-p-nitrophenylalanine-Arg(P2')-Leu(P3') has an Ala replacement at the P3 position and preferres such charged amino acid residue as Glu, Asp, Arg, or Lys at the P2' position

651761

additional information

Xanthomonas sp. T-22

651761

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Ca2+

Xanthomonas sp.

Ca2+-binding site

649090

additional information

Xanthomonas sp.

no inhibition or activation by divalent cations

137235

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INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1,2-epoxy-3-(4-nitrophenoxy)propane

chymostatin

weak

HgCl2

slight

isovaleryl-tyrosyl-leucyl-tyrosinal

Xanthomonas sp.

Q60106

tyrostatin

665088

Microbial alkaline protease inhibitor alpha

Xanthomonas sp.

weak

137235

PCMB

2 entries

additional information

4 entries

1,2-epoxy-3-(4-nitrophenoxy)propane

Pseudomonas sp.

137232

1,2-epoxy-3-(4-nitrophenoxy)propane

PCMB

slight

PCMB

slight

additional information

Pseudomonas sp.

diisopropyl fluorophosphate; EDTA; iodoacetic acid; methyl 2-diazoacetamidohexanoate; not: pepstatin; p-chloromercuribenzenesulfonic acid; phenylmethanesulfonyl fluoride

137232

additional information

Xanthomonas sp.

diazoacetyl-DL-norleucine methyl ester; divalent cations; methyl 2-diazoacetamidohexanoate; not: pepstatin

137235

additional information

Xanthomonas sp.

not inhibited by pepstatin. The hydrophilic nature of the S2' subsite appears to be a distinguishing feature of pepstatin-insensitive carboxyl proteinases

651761

additional information

Xanthomonas sp.

Q60106

no inhibition by pepstatin, 2,3-diamino naphthalene and 1,2-Epoxy-3-(p-nitrophenoxy)propane

665088

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0036

1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol

Xanthomonas sp.

Q60106

pH3.5, 37°C

665088

0.0188 - 0.0191

Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu

2 entries

0.00605

Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0058 - 0.0188

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

3 entries

0.0823 - 0.0829

Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu

2 entries

0.00932

Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0115

Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0139

Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.013

Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.012

Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.005

Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0135

Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00531

Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0026

Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0126

Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0174

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00763

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0063

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00995

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00824

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0106

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00746

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00982

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00944

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00532

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0183

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0213

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0217

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00977

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0283

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0158

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0164

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0138

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0101

Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00716

Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00214

Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0173

Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0662

Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00782

Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00523

Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00451

Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00445

Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0114

Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0183

Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0105

Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0039

Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0071

Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00224

Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.00995

Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.0188

Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu

Pseudomonas sp.

recombinant PCP

137236

0.0191

Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu

Pseudomonas sp.

autentic PCP

137236

0.0058

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C, mutant enzyme DELTA192

651950

0.0111

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C, mutant enzyme E230A

651950

0.0188

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C, mutant enzyme E235A

651950

0.0823

Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu

Pseudomonas sp.

recombinant PCP

137236

0.0829

Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu

Pseudomonas sp.

autentic PCP

137236

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

52.2

1-(4-methoxyphenyl)-2-methyl-3-nitro-1-H-indol-6-ol

Xanthomonas sp.

Q60106

pH3.5, 37°C

665088

20.4

Lys-Pro-Ala-Asp-Phe-(4-nitro)Phe-Arg-Leu

Pseudomonas sp.

autentic and recombinant PCP

137236

0.037 - 79.4

Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

0.3 - 68.4

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

5 entries

19.2

Lys-Pro-Ala-Lys-Phe-(4-nitro)Phe-Arg-Leu

Pseudomonas sp.

autentic and recombinant PCP

137236

105

Lys-Pro-Arg-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

38.3

Lys-Pro-Asn-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

18.1

Lys-Pro-Asp-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

2.2 - 73.9

Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

34 - 62.4

Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

60.5

Lys-Pro-Ile-Ala-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

43.3

Lys-Pro-Ile-Arg-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.8 - 35.7

Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

18.6

Lys-Pro-Ile-Asp-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

48.8

Lys-Pro-Ile-Gln-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

1.4

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ala-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

24.7

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ala

Xanthomonas sp.

pH 3.5, 37°C

651761

15.5

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Arg

Xanthomonas sp.

pH 3.5, 37°C

651761

101

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Asp

Xanthomonas sp.

pH 3.5, 37°C

651761

27.3

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ile

Xanthomonas sp.

pH 3.5, 37°C

651761

102

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

26.7

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Phe

Xanthomonas sp.

pH 3.5, 37°C

651761

1.6 - 71.3

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser

2 entries

27.6

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Tyr

Xanthomonas sp.

pH 3.5, 37°C

651761

30.9

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Val

Xanthomonas sp.

pH 3.5, 37°C

651761

32.7

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asn-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

20 - 87.9

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu

2 entries

189

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Glu-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

16.1

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ile-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Leu-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

43.7

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Lys-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

40.4

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Ser-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.04 - 8.57

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu

2 entries

16.9 - 23.1

Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

1.1 - 19.4

Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

16.8

Lys-Pro-Ile-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.97 - 16.6

Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

39.8

Lys-Pro-Ile-Nle-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

6 - 57.9

Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

56.8

Lys-Pro-Ile-Ser-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

30.5

Lys-Pro-Ile-Thr-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

15.9 - 26.9

Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

15.3

Lys-Pro-Leu-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

96.4

Lys-Pro-Lys-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

2.6 - 57.3

Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

3.4 - 36.7

Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu

2 entries

Lys-Pro-Thr-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

8.45

Lys-Pro-Tyr-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

28.1

Lys-Pro-Val-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.037 - 0.23

Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

79.4

Lys-Pro-Ala-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.3

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C, mutant enzyme E235A

651950

0.9

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C, mutant enzyme DELTA192

651950

21.8

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C, mutant enzyme E230A

651950

34.9

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C, mutant enzyme E235A

651950

68.4

Lys-Pro-Ala-Leu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C, mutant enzyme DELTA192

651950

2.2

Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

73.9

Lys-Pro-Gln-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

62.4

Lys-Pro-Glu-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.8

Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

35.7

Lys-Pro-Ile-Asn-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

1.6

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser

Xanthomonas sp.

pH 3.5, 37°C

651761

71.3

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Arg-Ser

Xanthomonas sp.

pH 3.5, 37°C

651761

20 - 50

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

87.9

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Asp-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.04 - 1.97

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

8.57

Lys-Pro-Ile-Glu-Phe-p-nitrophenylalanine-Val-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

16.9

Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

23.1

Lys-Pro-Ile-His-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

1.1

Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

19.4

Lys-Pro-Ile-Ile-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

0.97

Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

16.6

Lys-Pro-Ile-Lys-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

57.9

Lys-Pro-Ile-Pro-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

15.9

Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

26.9

Lys-Pro-Ile-Val-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

2.6

Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

57.3

Lys-Pro-Phe-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

3.4

Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

36.7

Lys-Pro-Ser-Glu-Phe-p-nitrophenylalanine-Arg-Leu

Xanthomonas sp.

pH 3.5, 37°C

651761

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Ki VALUE [mM]

INHIBITOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0000021

isovaleryl-tyrosyl-leucyl-tyrosinal

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

additional information

2 entries

additional information

Pseudomonas sp.

137232

additional information

Xanthomonas sp.

137235

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pH OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2.7

2 entries

Pseudomonas sp.

acid-denatured hemoglobin, casein

137232

2.7

Xanthomonas sp.

casein, acid-denatured hemoglobin

2.7

for casein digestion

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TEMPERATURE OPTIMUM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

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pI VALUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Pseudomonas sp.

2 entries

Pseudomonas sp. No. 101

brenda

brenda

expression in Escherichia coli

137236

brenda

Xanthomonas sp.

137235, 649090, 651950

brenda

T-22

brenda

T-22

SwissProt

brenda

T-22

brenda

T-22

SwissProt

brenda

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SOURCE TISSUE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

SOURCE

brenda

Go to AA Sequence and Transmembrane Helices SearchGo to Localization Prediction

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

Q60106 pBLAST

XANP_XANS2

Xanthomonas sp. (strain T-22)

827

83707

Swiss-Prot

Show Sequence

A0A378WBX6 pBLAST

A0A378WBX6_MYCFO

Mycolicibacterium fortuitum

537

56113

TrEMBL

Show Sequence

A0A839EVZ8 pBLAST

A0A839EVZ8_9GAMM

Dokdonella fugitiva

840

84494

TrEMBL

Show Sequence

Q396D3 pBLAST

Q396D3_BURL3

Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)

644

66322

TrEMBL

Show Sequence

A0A0P4UUC0 pBLAST

A0A0P4UUC0_9CYAN

Leptolyngbya sp. NIES-2104

275

29576

TrEMBL

Show Sequence

A0A2N7QNK5 pBLAST

A0A2N7QNK5_9GAMM

Dyella sp. AD56

579

60431

TrEMBL

Show Sequence

A0A2N7QQG1 pBLAST

A0A2N7QQG1_9GAMM

Dyella sp. AD56

886

91455

TrEMBL

Show Sequence

A0A7X0E2B7 pBLAST

A0A7X0E2B7_9GAMM

Rhodanobacter sp. MP1X3

872

89839

TrEMBL

Show Sequence

A0A2N9LE97 pBLAST

A0A2N9LE97_9BACT

Candidatus Sulfotelmatomonas gaucii

1198

119909

TrEMBL

Show Sequence

A0A0A1FCY2 pBLAST

A0A0A1FCY2_9BURK

Collimonas arenae

621

64173

TrEMBL

Show Sequence

A0A1M5VBF1 pBLAST

A0A1M5VBF1_9VIBR

Vibrio aerogenes CECT 7868

293

31038

TrEMBL

Show Sequence

Q7NWL8 pBLAST

Q7NWL8_CHRVO

Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

559

57414

TrEMBL

Show Sequence

A0A7W8LE23 pBLAST

A0A7W8LE23_9BURK

Paraburkholderia youngii

642

67617

TrEMBL

Show Sequence

A0A7Z7WE28 pBLAST

A0A7Z7WE28_9NEIS

Chromobacterium vaccinii

159

16820

TrEMBL

Show Sequence

A0A0A1F751 pBLAST

A0A0A1F751_9BURK

Collimonas arenae

635

65765

TrEMBL

Show Sequence

A0A1N7RST4 pBLAST

A0A1N7RST4_9BURK

Paraburkholderia ribeironis

682

72322

TrEMBL

Show Sequence

Q2SW22 pBLAST

Q2SW22_BURTA

Burkholderia thailandensis (strain ATCC 700388 / DSM 13276 / CIP 106301 / E264)

190

20028

TrEMBL

Show Sequence

Q7NWL5 pBLAST

Q7NWL5_CHRVO

Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757)

556

57810

TrEMBL

Show Sequence

Q08U45 pBLAST

Q08U45_STIAD

Stigmatella aurantiaca (strain DW4/3-1)

833

88623

TrEMBL

Show Sequence

A0A5P9J0N6 pBLAST

A0A5P9J0N6_9GAMM

Pseudoalteromonas sp. THAF3

877

94120

TrEMBL

Show Sequence

A0A8D3Y9M9 pBLAST

A0A8D3Y9M9_BURCM

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD)

774

78572

TrEMBL

Show Sequence

A0A238H6S4 pBLAST

A0A238H6S4_9BURK

Burkholderia singularis

598

62232

TrEMBL

Show Sequence

A0A2N7QXX0 pBLAST

A0A2N7QXX0_9GAMM

Dyella sp. AD56

748

77186

TrEMBL

Show Sequence

A0A2X1GH85 pBLAST

A0A2X1GH85_BURCE

Burkholderia cepacia

642

66250

TrEMBL

Show Sequence

A0A1V5G1L7 pBLAST

A0A1V5G1L7_9PROT

Alphaproteobacteria bacterium ADurb.BinA280

223

23015

TrEMBL

Show Sequence

A0A099KYA9 pBLAST

A0A099KYA9_COLPS

Colwellia psychrerythraea

901

99244

TrEMBL

Show Sequence

A0A099KB29 pBLAST

A0A099KB29_COLPS

Colwellia psychrerythraea

566

58625

TrEMBL

Show Sequence

A0A099L3G1 pBLAST

A0A099L3G1_COLPS

Colwellia psychrerythraea

717

75566

TrEMBL

Show Sequence

A0A0H2M505 pBLAST

A0A0H2M505_VARPD

Variovorax paradoxus

139

14821

TrEMBL

Show Sequence

A0A0A1FM98 pBLAST

A0A0A1FM98_9BURK

Collimonas arenae

661

68469

TrEMBL

Show Sequence

A0A2K9JZN2 pBLAST

A0A2K9JZN2_9GAMM

Pseudoalteromonas sp. NC201

654

71731

TrEMBL

Show Sequence

A0A841KPJ6 pBLAST

A0A841KPJ6_9GAMM

Oleiagrimonas soli

526

53189

TrEMBL

Show Sequence

A0A8B4QG75 pBLAST

A0A8B4QG75_MYCSM

Mycolicibacterium smegmatis

520

54602

TrEMBL

Show Sequence

R0DTC9 pBLAST

R0DTC9_RALPI

Ralstonia pickettii OR214

574

59970

TrEMBL

Show Sequence

A0A099L7S2 pBLAST

A0A099L7S2_COLPS

Colwellia psychrerythraea

1038

106089

TrEMBL

Show Sequence

A0A0A1FAY0 pBLAST

A0A0A1FAY0_9BURK

Collimonas arenae

628

64804

TrEMBL

Show Sequence

A0A846V8B2 pBLAST

A0A846V8B2_9GAMM

Dyella sp. SG609

876

90432

TrEMBL

Show Sequence

A0A7W5EMM9 pBLAST

A0A7W5EMM9_9GAMM

Luteibacter sp. Sphag1AF

825

83846

TrEMBL

Show Sequence

A0A381EWS5 pBLAST

A0A381EWS5_CHRVL

Chromobacterium violaceum

102

10398

TrEMBL

Show Sequence

A0A099K9R4 pBLAST

A0A099K9R4_COLPS

Colwellia psychrerythraea

687

71153

TrEMBL

Show Sequence

A0A238H2E5 pBLAST

A0A238H2E5_9BURK

Burkholderia singularis

634

65647

TrEMBL

Show Sequence

A0A846V057 pBLAST

A0A846V057_9GAMM

Dyella sp. SG609

798

82070

TrEMBL

Show Sequence

A0A2K8W4B5 pBLAST

A0A2K8W4B5_9GAMM

Dickeya solani RNS 08.23.3.1.A

623

67610

TrEMBL

Show Sequence

A0A7W8VB49 pBLAST

A0A7W8VB49_PARAM

Paraburkholderia atlantica

564

59328

TrEMBL

Show Sequence

Q393U2 pBLAST

Q393U2_BURL3

Burkholderia lata (strain ATCC 17760 / DSM 23089 / LMG 22485 / NCIMB 9086 / R18194 / 383)

577

60125

TrEMBL

Show Sequence

G0A9N7 pBLAST

G0A9N7_COLFT

Collimonas fungivorans (strain Ter331)

661

68367

TrEMBL

Show Sequence

Q0BAG7 pBLAST

Q0BAG7_BURCM

Burkholderia ambifaria (strain ATCC BAA-244 / AMMD)

579

60264

TrEMBL

Show Sequence

A0A1V6HFK8 pBLAST

A0A1V6HFK8_9BACT

Bacteroidetes bacterium ADurb.Bin041

433

48745

TrEMBL

Show Sequence

A0A0A1F9V7 pBLAST

A0A0A1F9V7_9BURK

Collimonas arenae

113

11932

TrEMBL

Show Sequence

I7FYH5 pBLAST

I7FYH5_MYCS2

Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155)

550

57980

TrEMBL

Show Sequence

A0A7X0E8E7 pBLAST

A0A7X0E8E7_9GAMM

Rhodanobacter sp. A1T4

878

90410

TrEMBL

Show Sequence

A0A099KUA5 pBLAST

A0A099KUA5_COLPS

Colwellia psychrerythraea

690

73374

TrEMBL

Show Sequence

A0A099KY20 pBLAST

A0A099KY20_COLPS

Colwellia psychrerythraea

984

106609

TrEMBL

Show Sequence

A0A1M7YWI3 pBLAST

A0A1M7YWI3_9VIBR

Vibrio quintilis

292

31153

TrEMBL

Show Sequence

A0A378W4S7 pBLAST

A0A378W4S7_9MYCO

Mycobacterium senegalense

543

56851

TrEMBL

Show Sequence

A0A841KE35 pBLAST

A0A841KE35_9GAMM

Oleiagrimonas soli

538

54545

TrEMBL

Show Sequence

A0A8D9NRL7 pBLAST

A0A8D9NRL7_BURPE

Burkholderia pseudomallei

644

66625

TrEMBL

Show Sequence

A0A2K9K1W2 pBLAST

A0A2K9K1W2_9GAMM

Pseudoalteromonas sp. NC201

600

63986

TrEMBL

Show Sequence

A9F4F7 pBLAST

A9F4F7_SORC5

Sorangium cellulosum (strain So ce56)

654

68201

TrEMBL

Show Sequence

A0A4Q4DN59 pBLAST

A0A4Q4DN59_9ACTN

Streptomyces sp. L-9-10

157

17141

TrEMBL

Show Sequence

A0A846UVE5 pBLAST

A0A846UVE5_9GAMM

Dyella sp. SG609

536

54417

TrEMBL

Show Sequence

A0A2K9K4P9 pBLAST

A0A2K9K4P9_9GAMM

Pseudoalteromonas sp. NC201

868

93727

TrEMBL

Show Sequence

A0A099L2J5 pBLAST

A0A099L2J5_COLPS

Colwellia psychrerythraea

831

86904

TrEMBL

Show Sequence

A0A5P9EYH8 pBLAST

A0A5P9EYH8_9GAMM

Microbulbifer sp. THAF38

740

81903

TrEMBL

Show Sequence

A0A238HBD3 pBLAST

A0A238HBD3_9BURK

Burkholderia singularis

584

61272

TrEMBL

Show Sequence

A0A509E2B2 pBLAST

A0A509E2B2_9HYPH

Thalassocella blandensis

527

55881

TrEMBL

Show Sequence

A0A0H5CSR0 pBLAST

A0A0H5CSR0_9PSEU

Alloactinosynnema sp. L-07

188

18697

TrEMBL

Show Sequence

A0A0H2XXD6 pBLAST

A0A0H2XXD6_BURCA

Burkholderia cenocepacia (strain AU 1054)

577

60264

TrEMBL

Show Sequence

A0A8E0L4G0 pBLAST

A0A8E0L4G0_BURTH

Burkholderia thailandensis MSMB43

554

57464

TrEMBL

Show Sequence

A0A833QE37 pBLAST

A0A833QE37_BURGA

Burkholderia gladioli

668

68738

TrEMBL

Show Sequence

A4JM62 pBLAST

A4JM62_BURVG

Burkholderia vietnamiensis (strain G4 / LMG 22486)

579

60511

TrEMBL

Show Sequence

A0A839V1Z0 pBLAST

A0A839V1Z0_9PROT

Endobacter medicaginis

614

63256

TrEMBL

Show Sequence

A0A0P9G9X3 pBLAST

A0A0P9G9X3_9GAMM

Pseudoalteromonas sp. P1-9

867

94721

TrEMBL

Show Sequence

Entries 1 - 20 of 73

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

40000

gel filtration

41000

42000

gel filtration

43000

1 * 43000, SDS-PAGE

61070

calculation from PCP gene, processed and secreted into the periplasm as a 43000 protein, which, under acidic conditions converts to mature PCP

137236

84000

Xanthomonas sp.

Q60106

recombinant protein from Escherichia coli

41000

1 * 41000, SDS-PAGE

41000

2 * 41000, SDS-PAGE

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

dimer

monomer

dimer

2 * 41000, SDS-PAGE

dimer

2 * 41000, SDS-PAGE

monomer

Pseudomonas sp.

1 * 43000, SDS-PAGE

137232

monomer

Pseudomonas sp. No. 101

1 * 43000, SDS-PAGE

monomer

Xanthomonas sp.

1 * 41000, SDS-PAGE

137235

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POSTTRANSLATIONAL MODIFICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

proteolytic modification

Xanthomonas sp.

N-terminal propeptide and C-terminal propeptide

649090

additional information

3 entries

additional information

Pseudomonas sp.

contains less than 0.05% carbohydrate

137232

additional information

Pseudomonas sp. No. 101

contains less than 0.05% carbohydrate

additional information

Xanthomonas sp.

contains less than 0.05% carbohydrate

137235

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

Xanthomonas sp.

649090

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

D348A

Xanthomonas sp.

production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected

651950

DELTA192

Xanthomonas sp.

pro-region mutant enzyme

651950

S169A

Xanthomonas sp.

production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected

651950

S79A

Xanthomonas sp.

production of a 64000 Da precursor protein which is not processed to active enzymes under acidic conditions, no catalytic acticity detected

651950

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

3.5 - 5.3

Xanthomonas sp.

37°C, 20 h, stable

137235

4 - 5.5

Pseudomonas sp.

37°C, 15 h, stable

137232

4.8

Pseudomonas sp.

15 min, stable below 50°C, complete loss of activity at 60°C

137232

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TEMPERATURE STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

15 min, stable below

pH 4.8, 15 min, stable below

137235

Xanthomonas sp.

pH 4.8, 15 min, complete loss of activity

137235

Pseudomonas sp.

15 min, complete loss of activity

137232

Pseudomonas sp.

pH 4.0-5.5, 15 h, stable

137232

Xanthomonas sp.

pH 3.5-5.3, 20 h, stable

137235

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

Xanthomonas sp.

137235

recombinant mutant enzymes S79A, S169A and D348A

strain No. 101

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expression in Escherichia coli

3 entries

expression in Escherichia coli

Pseudomonas sp.

137236

expression in Escherichia coli

Xanthomonas sp.

651950

expression in Escherichia coli

Xanthomonas sp.

Q60106

665088

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

137232

Oda, K.; Sugitani, M.; Fukuhara, K.; Murao, S.

Purification and properties of a pepstatin-insensitive carboxyl proteinase from a gram-negative bacterium

Biochim. Biophys. Acta

923

463-469

1987

Pseudomonas sp., Pseudomonas sp. No. 101

3548827

brenda

137235

Oda, K.; Nakazima, T.; Terashita, T.; Suzuki, K.i.; Murao, S.

Purification and properties of an S-PI(pepstatin Ac)-insensitive carboxyl proteinase from a Xanthomonas sp. Bacterium

Agric. Biol. Chem.

3073-3080

1987

Xanthomonas sp.

brenda

137236

Oda, K.; Takahashi, T.; Tokuda, Y.; Shibano, Y.; Takahashi, S.

Cloning, nucleotide sequence, and expression of an isovaleryl pepstatin-insensitive carboxyl proteinase gene from Pseudomonas sp. 101

J. Biol. Chem.

269

26518-26524

1994

Pseudomonas sp., Pseudomonas sp. No. 101

7929375

brenda

649090

Wlodawer, A.; Li, M.; Gustchina, A.; Oyama, H.; Dunn, B.M.; Oda, K.

Structural and enzymatic properties of the sedolisin family of serine-carboxyl peptidases

Acta Biochim. Pol.

81-102

2003

Xanthomonas sp.

12673349

brenda

651761

Narutaki, S.; Dunn, B.M.; Oda, K.

Subsite preferences of pepstatin-insensitive carboxyl proteinases from bacteria

J. Biochem.

125

75-81

1999

Xanthomonas sp., Xanthomonas sp. T-22

9880800

brenda

651950

Oyama, H.; Abe, S.I.; Ushiyama, S.; Takahashi, S.; Oda, K.

Identification of catalytic residues of pepstatin-insensitive carboxyl proteinases from prokaryotes by site-directed mutagenesis

J. Biol. Chem.

274

27815-27822

1999

Xanthomonas sp.

10488127

brenda

665088

Oda, K.

Sedolisin-B

Handbook of proteolytic enzymes (Barrett, A. J. , Rawlings, N. D. , Woessner, J. F. , eds. ) Academic Press

1888-1889

2004

Xanthomonas sp. (Q60106), Xanthomonas sp. T-22 (Q60106)

brenda

Entries 1 - 20 of 7

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EXTERNAL LINKS (specific for EC-Number 3.4.21.101)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

MetaCyc

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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