Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.21.10 - acrosin and Organism(s) Bos taurus and UniProt Accession P79343

for references in articles please use BRENDA:EC3.4.21.10
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.21 Serine endopeptidases
                3.4.21.10 acrosin
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Bos taurus
UNIPROT: P79343 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Bos taurus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
hide
preferential cleavage: Arg-/-, Lys-/-
Synonyms
acrosin, sperm acrosin, beta-acrosin, alpha-acrosin, acrosomal proteinase, acrosomal protease, acrosin amidase, acrosin ii, psi-acrosin, acrosin i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acrosin
trypsin-like serine protease of the S1 family
53 kDa fucose-binding protein
-
-
-
-
acrosin amidase
-
-
-
-
acrosomal protease
-
-
-
-
acrosomal proteinase
-
-
-
-
acrozonase
-
-
-
-
alpha-acrosin
-
-
-
-
beta-acrosin
-
-
-
-
proteinase, acrosomal
-
-
-
-
psi-acrosin
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9068-57-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
proacrosin + H2O
acrosin + peptide
show the reaction diagram
acrosin autoactivates through an endoproteolytic cleavage at the N-terminus with subsequent conversion to the mature enzyme through C-terminus cleavages
-
-
?
N-alpha-benzoyl-DL-arginine-4-nitroanilide + H2O
N-alpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-DL-arginine 4-nitroanilide + H2O
Nalpha-benzoyl-DL-arginine + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Nalpha-benzoyl-L-arginine ethyl ester + H2O
?
show the reaction diagram
-
-
-
-
?
proacrosin + H2O
acrosin + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
proacrosin + H2O
acrosin + peptide
show the reaction diagram
acrosin autoactivates through an endoproteolytic cleavage at the N-terminus with subsequent conversion to the mature enzyme through C-terminus cleavages
-
-
?
proacrosin + H2O
acrosin + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dacarbazine
-
BIC
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
lysophosphatidylcholine
-
in heparin-capacitated spermatozoa, the addition of lysophosphatidylcholine produces a significant increase (about 7fold) in acrosin activity
progesterone
-
up to 2fold activation
additional information
-
not activating: heparin, quercitin
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
92.9
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
bovine proacrosine
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
initially present in the sperm acrosomal vesicle of mammalian spermatozoa as the zymogen form, proacrosin, converted to the active form during the acrosome reaction, after which most acrosin molecules are released from the acrosomal vesicle, with a portion remaining associated with the sperm
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
acrosin is responsible for the serine protease activity associated with the inner acrosomal membrane
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
P79343_BOVIN
377
0
41722
TrEMBL
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41722
x * 41722, calculated from the deduced amino acid sequence
35000
-
x * 35000, mature form, SDS-PAGE
39000
-
x * 39000, SDS-PAGE
43000
-
x * 43000, immmature form, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 41722, calculated from the deduced amino acid sequence
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
96% of acrosin of capacitated sperm samples and control is present in the zymogen form
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Elce, J.S.; McIntyre, E.J.
Purification of bovine and human acrosin
Can. J. Biochem.
60
8-14
1981
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Palencia, D.D.; Garner, D.L.; Hudig, D.; Holcombe, D.W.; Burner, C.A.; Redelman, D.; Fernandez, G.C.J.; Abuelyaman, A.S.; Kam, C.M.; Powers, J.C.
Determination of activable proacrosin/acrosin in bovine sperm using an irreversible isocoumarin serine protease inhibitor
Biol. Reprod.
55
536-542
1996
Bos taurus
Manually annotated by BRENDA team
NagDas, S.K.
Bovine epididymal sperm proacrosin-acrosin system: quantification and partial characterization
Andrologia
24
171-178
1992
Bos taurus
Manually annotated by BRENDA team
Rosatti, M.I.; Beconi, M.T.; Cordoba, M.
Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen
Biocell
28
311-316
2004
Bos taurus
Manually annotated by BRENDA team
Raterman, D.; Springer, M.S.
The molecular evolution of acrosin in placental mammals
Mol. Reprod. Dev.
75
1196-1207
2008
Amblysomus hottentotus (B0LM06), Bos taurus (P79343), Bradypus tridactylus (B0LM16), Canis lupus familiaris, Cavia porcellus (Q60491), Cynocephalus volans (B0LM30), Diceros bicornis (B0LM26), Dugong dugon (B0LM13), Echinops telfairi (B0LM07), Elephantulus edwardii (B0LM09), Equus caballus (B0LM27), Erethizon dorsatum (B0LM36), Euphractus sexcinctus (B0LM17), Felis catus (B0LM18), Galegeeska rufescens (B0LM10), Homo sapiens (P10323), Homo sapiens, Hystrix brachyura (B0LM35), Lama glama (B0LM21), Loxodonta africana (B0LM12), Macaca mulatta, Macroscelides proboscideus (B0LM11), Manis sp. DMR-2008 (B0LM29), Mus musculus (Q3ZB05), Ochotona princeps (B0LM32), Orycteropus afer (B0LM15), Oryctolagus cuniculus (P48038), Otospermophilus beecheyi (B0LM38), Ovis aries (Q9GL10), Pan troglodytes (B0LM34), Procavia capensis (B0LM08), Puma concolor (B0LM19), Rattus fuscipes (B0LM37), Rattus norvegicus (P29293), Sorex cinereus (B0LM24), Sus scrofa (P08001), Sylvilagus floridanus (B0LM33), Tadarida brasiliensis (B0LM23), Talpa europaea (B0LM25), Tapirus pinchaque (B0LM28), Tragelaphus angasii (B0LM22), Trichechus manatus (B0LM14), Tupaia glis (B0LM31), Tursiops truncatus (B0LM20)
Manually annotated by BRENDA team
Ferrer, M.; Rodriguez, H.; Zara, L.; Yu, Y.; Xu, W.; Oko, R.
MMP2 and acrosin are major proteinases associated with the inner acrosomal membrane and may cooperate in sperm penetration of the zona pellucida during fertilization
Cell Tissue Res.
349
881-895
2012
Bos taurus
Manually annotated by BRENDA team
Perez Aguirreburualde, M.S.; Fernandez, S.; Cordoba, M.
Acrosin activity regulation by protein kinase C and tyrosine kinase in bovine sperm acrosome exocytosis induced by lysophosphatidylcholine
Reprod. Domest. Anim.
47
915-920
2012
Bos taurus
Manually annotated by BRENDA team