Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.4.19.9 - folate gamma-glutamyl hydrolase and Organism(s) Arabidopsis thaliana and UniProt Accession O65355

for references in articles please use BRENDA:EC3.4.19.9
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.9 folate gamma-glutamyl hydrolase
IUBMB Comments
The enzyme, which occurs only in animals and plants, can be either endo- and/or exopeptidase. It acts on tetrahydropteroyl polyglutamates and their modified forms, as well as the polyglutamates of the folate breakdown product N-(4-aminobenzoyl)-L-glutamate (pABA-Glu). The initial cleavage may release either monoglutamate or poly-gamma-glutamate of two or more residues, depending on the specific enzyme. For example, GGH1 from the plant Arabidopsis thaliana cleaves pentaglutamates, mainly to di- and triglutamates, whereas GGH2 from the same organism yields mainly monoglutamates. The enzyme is lysosomal (and secreted) in animals and vacuolar in plants. In peptidase family C26.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: O65355
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
conjugase, gamma-glutamyl hydrolase, folate conjugase, folylpolyglutamate hydrolase, gamma-gh, leggh2, leggh3, zgammagh, leggh1, low gamma-glutamyl hydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxypeptidase G
-
-
-
-
conjugase
-
-
-
-
FGPH
-
-
-
-
folate conjugase
-
-
-
-
folic acid conjugase
-
-
-
-
folylpolyglutamate hydrolase
-
-
-
-
gamma-Glu-X carboxypeptidase
-
-
-
-
gamma-glutamyl hydrolase
-
-
-
-
hydrolase, gamma-glutamyl
-
-
-
-
lysosomal gamma-glutamyl carboxypeptidase
-
-
-
-
poly(gamma-glutamic acid) endohydrolase
-
-
-
-
poly(glutamic acid) hydrolase II
-
-
-
-
polyglutamate hydrolase
-
-
-
-
pteroyl-poly-gamma-glutamate hydrolase
-
-
-
-
pteroylpoly-gamma-glutamyl hydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
tetrahydropteroyl-poly-gamma-glutamyl gamma-glutamyl hydrolase
The enzyme, which occurs only in animals and plants, can be either endo- and/or exopeptidase. It acts on tetrahydropteroyl polyglutamates and their modified forms, as well as the polyglutamates of the folate breakdown product N-(4-aminobenzoyl)-L-glutamate (pABA-Glu). The initial cleavage may release either monoglutamate or poly-gamma-glutamate of two or more residues, depending on the specific enzyme. For example, GGH1 from the plant Arabidopsis thaliana cleaves pentaglutamates, mainly to di- and triglutamates, whereas GGH2 from the same organism yields mainly monoglutamates. The enzyme is lysosomal (and secreted) in animals and vacuolar in plants. In peptidase family C26.
CAS REGISTRY NUMBER
COMMENTARY hide
9074-87-7
not distinguishable from EC 3.4.17.11 in Chemical Abstracts
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5,10-methenyltetrahydrofolate glutamate + H2O
5,10-methenyltetrahydrofolate + L-glutamate
show the reaction diagram
-
-
-
?
5,10-methenyltetrahydrofolate heptaglutamate + 7 H2O
5,10-methenyltetrahydrofolate + 7 L-glutamate
show the reaction diagram
-
-
-
?
5,10-methenyltetrahydrofolate hexaglutamate + 6 H2O
5,10-methenyltetrahydrofolate + 6 L-glutamate
show the reaction diagram
-
-
-
?
5-formyltetrahydrofolate glutamate + H2O
5-formyltetrahydrofolate + L-glutamate
show the reaction diagram
-
-
-
?
5-formyltetrahydrofolate heptaglutamate + 7 H2O
5-formyltetrahydrofolate + 7 L-glutamate
show the reaction diagram
-
-
-
?
5-formyltetrahydrofolate hexaglutamate + 6 H2O
5-formyltetrahydrofolate + 6 L-glutamate
show the reaction diagram
-
-
-
?
5-methyltetrahydrofolate glutamate + H2O
5-methyltetrahydrofolate + L-glutamate
show the reaction diagram
-
-
-
?
5-methyltetrahydrofolate heptaglutamate + 7 H2O
5-methyltetrahydrofolate + 7 L-glutamate
show the reaction diagram
-
-
-
?
5-methyltetrahydrofolate hexaglutamate + 6 H2O
5-methyltetrahydrofolate + 6 L-glutamate
show the reaction diagram
-
-
-
?
p-aminobenzoyl-penta-gamma-glutamate + H2O
?
show the reaction diagram
pteroyl-penta-gamma-glutamate + H2O
?
show the reaction diagram
additional information
?
-
isoform GGH2 alone is able to completely deconjugate folates using 50-100 microg/g of sample for 1 h at 37°C in all matrices tested. It removes the tails of folylpolyglutamates from tomato fruit, black bean and peanut seeds, and alfalfa sprouts extracts
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0015 - 0.00186
p-aminobenzoylpentaglutamate
0.00052 - 0.00079
pteroylpentaglutamate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.51 - 8.29
p-aminobenzoylpentaglutamate
0.04 - 19.46
pteroylpentaglutamate
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GGH2_ARATH
347
0
38642
Swiss-Prot
Secretory Pathway (Reliability: 1)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant AtGGH1
recombinant AtGGH2
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtGGH1 is expressed in truncated form in Escherichia coli
AtGGH2 is expressed in truncated form in Escherichia coli
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
folate analysis. Isoform GGH2 is able to completely deconjugate folates using 50-100 microg/g of sample for 1 h at 37°C in all matrices tested. It removes the tails of folylpolyglutamates from tomato fruit, black bean and peanut seeds, and alfalfa sprouts extracts
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Orsomando, G.; de la Garza, R.D.; Green, B.J.; Peng, M.; Rea, P.A.; Ryan, T.J.; Gregory, J.F.; Hanson, A.D.
Plant gamma-glutamyl hydrolases and folate polyglutamates: characterization, compartmentation, and co-occurrence in vacuoles
J. Biol. Chem.
280
28877-28884
2005
Arabidopsis thaliana (O65355), Beta vulgaris, Pisum sativum
Manually annotated by BRENDA team
Ramos-Parra, P.; Urrea-Lopez, R.; Diaz de la Garza, R.
Folate analysis in complex food matrices: Use of a recombinant Arabidopsis ?-glutamyl hydrolase for folate deglutamylation
Food Res. Int.
54
177-185
2013
Arabidopsis thaliana (O65355)
Manually annotated by BRENDA team