Information on EC 3.4.19.2 - peptidyl-glycinamidase

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The expected taxonomic range for this enzyme is: Tetrapoda

EC NUMBER
COMMENTARY hide
3.4.19.2
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RECOMMENDED NAME
GeneOntology No.
peptidyl-glycinamidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cleavage of C-terminal glycinamide from polypeptides
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
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CAS REGISTRY NUMBER
COMMENTARY hide
94047-14-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
toad
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-L-Trp ethyl ester + H2O
?
show the reaction diagram
toad
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-
-
-
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alpha-aminosuberic acid oxytocin + H2O
glycinamide + ?
show the reaction diagram
-
-
-
?
alpha-deamino-cystathionine-6,1-oxytocin + H2O
glycinamide + ?
show the reaction diagram
-
-
-
?
antidiuretic hormone + H2O
glycinamide + ?
show the reaction diagram
-
release of glycinamide from the C-terminus of the hormone
-
?
benzoyl-L-Arg ethyl ester + H2O
?
show the reaction diagram
toad
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-
-
-
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beta-deamino-cystathionine-1,6-oxytocin + H2O
glycinamide + ?
show the reaction diagram
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-
-
?
oxytocin + H2O
?
show the reaction diagram
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the enzyme is not a strict peptidyl-glycinamidase, but can release other amino acid amides from a variety of amidated peptide
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-
?
oxytocin + H2O
glycinamide + ?
show the reaction diagram
Vasopressin + H2O
?
show the reaction diagram
-
-
-
-
?
[Arg8]-vasopressin + H2O
?
show the reaction diagram
toad
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hydrolysis of the Arg8-Gly9-NH2 bond
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-
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additional information
?
-
toad
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enzyme inactivates neurophyseal hormones
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
toad
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enzyme inactivates neurophyseal hormones
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-
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-methylumbelliferyl p-guanidinobenzoate
toad
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alpha-aminosuberic acid oxytocin
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at high concentrations
antipain
toad
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beta-deamino-cystathionine-1,6-oxytocin
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at high concentrations
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Ca2+
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strong inhibition
chymostatin
toad
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concanavalin A
toad
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diisopropyl phosphofluoridate
Elastatinal
toad
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leupeptin
toad
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Lima bean trypsin inhibitor
toad
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Mg2+
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slight inhibition
ovoinhibitor
toad
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ovomucoid
toad
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Oxytocin
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p-Nitrophenyl p-guanidinobenzoate
toad
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tosyllysine chloromethyl ketone
toad
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tosylphenylalanine chloromethyl ketone
toad
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Trasylol
toad
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0025 - 0.0033
alpha-aminosuberic acid
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0.0025 - 0.0033
alpha-deamino-cystathionine
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0.00625
beta-deamino-cystathionine-1,6-oxytocin
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-
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0.0025 - 0.0033
Oxytocin
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
toad
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hydrolysis of 8-arginine-vasopressin or benzoyl-L-Arg ethyl ester
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
medulla
Manually annotated by BRENDA team
toad
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
toad
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gel filtration
442000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
EDTA, 0.001 mM, stabilizes during purification
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
to homogeneity
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