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Information on EC 3.4.19.12 - ubiquitinyl hydrolase 1 and Organism(s) Sus scrofa and UniProt Accession Q06AB3

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.19 Omega peptidases
                3.4.19.12 ubiquitinyl hydrolase 1
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This record set is specific for:
Sus scrofa
UNIPROT: Q06AB3 not found.
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Word Map
The taxonomic range for the selected organisms is: Sus scrofa
The enzyme appears in selected viruses and cellular organisms
Synonyms
uch-l1, uchl1, ataxin-3, deubiquitinating enzyme, pgp 9.5, usp22, pgp9.5, plpro, otub1, isopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
AD-019
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-
-
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CGI-70
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-
-
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esterase, ubiquitin thiol-
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-
-
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hydrolase, ubiquitin carboxyl-terminal
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-
-
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hydrolase, ubiquitin carboxyl-terminal (Aplysia californica gene Ap-uch)
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-
-
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isopeptidase
-
-
-
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isopeptidase T
-
-
-
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Neuron cytoplasmic protein 9.5
-
-
-
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PGP 9.5
-
-
-
-
PGP9.5
ubiquitin C-terminal hydrolase
ubiquitin C-terminal hydrolase (Aplysia californica gene Ap-uch)
-
-
-
-
ubiquitin C-terminal hydrolase L1
-
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Ubiquitin C-terminal hydrolase UCH37
-
-
-
-
ubiquitin carboxy-terminal hydrolase
-
-
-
-
ubiquitin carboxy-terminal hydrolase 1
-
-
ubiquitin carboxyl-terminal hydrolase
-
-
-
-
Ubiquitin thiolesterase
-
-
-
-
Ubiquitin thiolesterase L1
-
-
-
-
Ubiquitin thiolesterase L3
-
-
-
-
Ubiquitin thiolesterase L4
-
-
-
-
Ubiquitin thiolesterase L5
-
-
-
-
ubiquitin-carboxyl-terminal hydrolase PGP-9.5
-
-
-
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UCH-1
-
-
-
-
UCH-L1
UCH-L2
-
-
-
-
UCH-L3
-
-
-
-
UCH-L4
-
-
-
-
UCH-L5
-
-
-
-
UCHL1
-
-
Uchl3
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-
yeast ubiquitin hydrolase
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-
-
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YUH1
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
189642-63-5
-
86480-67-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ubiquitin-7-amido-4-methylcoumarin + H2O
ubiquitin + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
additional information
?
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UCHL3 interacts with peripheral acrosomal plasma membrane proteins
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-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Antibody
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anti-UCH antibodies increase the rate of polyspermy during in vitro fertilization by reducing UCH enzymatic activity
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Ubiquitin aldehyde
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specific nonpermeating UCH inhibitor, signficantly increases rates of sperm-zona pellucida penetration and polyspermy during in vitro fertilization by reducing UCH enzymatic activity in motile boar spermatozoa. Ipase-T and ubiquitin aldehyde cancel each other out when added concomitantly to the fertilization medium
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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UCHL3
Manually annotated by BRENDA team
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UCHL3 is localized to the acrosomal surface. UCHL1 is absent from the sperm surface
Manually annotated by BRENDA team
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UCHL3 mainly localized to the round spermatids (acrosomal cap) and elongating spermatids (caudal manchette and acrosome)
Manually annotated by BRENDA team
additional information
-
in pigs, gonocytes and spermatogonia expressing UCH-L1 include the population expressing PLZF, a known determinant of undifferentiated spermatogonial stem cells, asymmetric segregation of UCH-L1 and PLZF in spermatogonia in vivo and in vitro, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
UCHL3 diffusely distributed in the zygotic cytoplasm after fertilization
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
UCHL3_PIG
230
0
26090
Swiss-Prot
other Location (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
-
UCHL1 or UCHL3, Western blot analysis, immunocytochemistry
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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sperm acrosomal UCHs are involved in sperm-zona pellucida interactions and antipolyspermy defense. Modulation of spermal UCH activity may facilitate the management of polyspermy during in vitro fertilization and provide insights into male fertility. Recombinant UCHs reduce polyspermy and proportionally increase the rate of monospermic fertilization during in vitro fertilization. Oocyte-secreted UCHs are not necessary for antipolyspermy defense during in vitro fertilization
additional information
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essential role of UCH-L1 in oocyte maturation and in the completion of the first meiosis and its transition to anaphase
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yi, Y.J.; Manandhar, G.; Sutovsky, M.; Li, R.; Jonakova, V.; Oko, R.; Park, C.S.; Prather, R.S.; Sutovsky, P.
Ubiquitin C-terminal hydrolase-activity is involved in sperm acrosomal function and anti-polyspermy defense during porcine fertilization
Biol. Reprod.
77
780-793
2007
Sus scrofa
Manually annotated by BRENDA team
Susor, A.; Ellederova, Z.; Jelinkova, L.; Halada, P.; Kavan, D.; Kubelka, M.; Kovarova, H.
Proteomic analysis of porcine oocytes during in vitro maturation reveals essential role for the ubiquitin C-terminal hydrolase-L1
Reproduction
134
559-568
2007
Sus scrofa
Manually annotated by BRENDA team
Luo, J.; Megee, S.; Dobrinski, I.
Asymmetric distribution of UCH-L1 in spermatogonia is associated with maintenance and differentiation of spermatogonial stem cells
J. Cell. Physiol.
220
460-468
2009
Sus scrofa
Manually annotated by BRENDA team