Information on EC 3.4.19.11 - gamma-D-glutamyl-meso-diaminopimelate peptidase

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The expected taxonomic range for this enzyme is: Lysinibacillus sphaericus

EC NUMBER
COMMENTARY hide
3.4.19.11
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RECOMMENDED NAME
GeneOntology No.
gamma-D-glutamyl-meso-diaminopimelate peptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of gamma-D-glutamyl bonds to the L-terminus (position 7) of meso-diaminopimelic acid (meso-A2pm) in 7-(L-Ala-gamma-D-Glu)-meso-A2pm and 7-(L-Ala-gamma-D-Glu)-7-(D-Ala)-meso-A2pm. It is required that the D-terminal amino and carboxy groups of meso-A2pm are unsubstituted
show the reaction diagram
position 7
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
62572-28-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
N-Acetylglucosamine-N-acetylmuramoyl-L-Ala-D-Glu-[-(L)-meso-diaminopimelic acid-D-Ala]NH2 + H2O
?
show the reaction diagram
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N-Acetylmuramoyl-L-Ala-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala + H2O
N-Acetylmuramoyl-L-Ala-D-Glu + meso-diaminopimelic acid-(L)-D-Ala
show the reaction diagram
N-Acetylmuramoyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala + H2O
N-Acetylmuramoyl-L-Ala-gamma-D-Glu + meso-diaminopimelic acid-(L)-D-Ala
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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activates apoenzyme, maximal activation at 0.0002 mM
Mn2+
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activates apoenzyme, maximal activation at 0.0001 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DD-Diaminopimelic acid isomers
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Dicarboxylic D-amino acids
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inhibition is a function of length, maximum at n=4
EDTA
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reactivation by zinc, cobalt and manganese ions
meso-diaminopimelic acid isomers
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o-phenanthroline
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reactivation by zinc, cobalt and manganese ions
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Detergents
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enhance activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.99 - 4.34
N-acetylglucosamine-N-acetylmuramoyl-L-Ala-D-Glu-[-(L)-meso-diaminopimelic acid-D-Ala]NH2
0.33
N-acetylmuramoyl-L-Ala-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala
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0.57 - 0.8
N-acetylmuramoyl-L-Ala-gamma-D-Glu-(L)-meso-diaminopimelic acid-(L)-D-Ala
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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enzyme from sporulation medium
13.9
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enzyme from spores
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8
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native and immobilized enzyme
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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sporulation medium
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Mycobacterium paratuberculosis (strain ATCC BAA-968 / K-10)
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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stable for 45 days
55
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10 min, 2% loss of activity
65
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10 min, 3% loss of activity
85
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10 min, 88% loss of activity
95
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10 min, 99% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, stable for months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Show AA Sequence (167 entries)
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