Information on EC 3.4.17.B6 - tubulinyl-Glu carboxypeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.17.B6
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
tubulinyl-Glu carboxypeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
cleavage of gamma-linked Glu residues from the side chain of alpha- and beta-tubulin
show the reaction diagram
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-Tubulin + H2O
?
show the reaction diagram
-
-
treatment with isoform CCP5 leads to a decrease in the peak intensities of the multi- as well as monoglutamylated alpha-tubulin forms and an increase in the peak intensity of the form lacking all Glu residues on the side chain
-
?
alpha-tubulin + H2O
DELTA2-tubulin + ?
show the reaction diagram
beta2b-tubulin + H2o
?
show the reaction diagram
-
-
incubation with isoform CCP5 causes decreases of the peaks corresponding to multi- and monoglutamylated beta2b-tubulin and a dramatic increase of the peak corresponding to beta2b-tubulin without Glu on the side chain
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?
DATAEEEGEMYE(gamma-(E)2)-DDDEESEAQGPK + 2 H2O
DATAEEEGEMYEDDDEESEAQGPK + 2 L-Glu
show the reaction diagram
-
synthetic beta3-tubulin-based 24-residue C-terminal peptide with two side chain Glu residues
about 30% removal of Glu side chains
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?
DATAEEEGEMYE(gamma-(E)3)-DDDEESEAQGPK + 3 H2O
DATAEEEGEMYEDDDEESEAQGPK + 3 L-Glu
show the reaction diagram
-
synthetic beta3-tubulin-based 24-residue C-terminal peptide with three side chain Glu residues
-
-
?
DATAEEEGEMYE(gamma-(E)4)-DDDEESEAQGPK + 4 H2O
DATAEEEGEMYEDDDEESEAQGPK + 4 L-Glu
show the reaction diagram
-
synthetic beta3-tubulin-based 24-residue C-terminal peptide with four side chain Glu residues
-
-
?
DATAEEEGEMYE(gamma-E)-DDDEESEAQGPK + H2O
DATAEEEGEMYEDDDEESEAQGPK + L-Glu
show the reaction diagram
-
synthetic beta3-tubulin-based 24-residue C-terminal peptide with one side chain Glu residue
complete removal of Glu side chain
-
?
DELTA2-tubulin + H2O
DELTA3-tubulin + ?
show the reaction diagram
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i.e. tubulin lacking two C-terminal amino acids
i.e. tubulin lacking two C-terminal amino acids
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?
monoglutamyl alpha-tubulin + H2O
alpha-tubulin + L-Gln
show the reaction diagram
-
-
-
r
monoglutamyl beta-tubulin + H2O
beta-tubulin + L-Gln
show the reaction diagram
-
-
-
r
polymerized microtubules + H2O
?
show the reaction diagram
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polymerized microtubules prepared from MDA-MB-231 cells grown on a coverslip. Isoform CCP5 eliminates GT335 signals and diminishes poly(E) signals on polymerized tubulin
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-
?
polymerized microtubules + H2O
DELTA2-tubulin + ?
show the reaction diagram
tubulin + H2O
?
show the reaction diagram
-
porcine brain tubulin. Isoform CCP5 can process paclitaxel-treated tubulin, and the activity of CCP5 in the presence of paclitaxel is comparable with the activity in the absence of the drug
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?
additional information
?
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Sf9 cell
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