Information on EC 3.4.17.B5 - Pyrococcus furiosus carboxypeptidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Pyrococcus furiosus

EC NUMBER
COMMENTARY hide
3.4.17.B5
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Pyrococcus furiosus carboxypeptidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
broad specificity for neutral, aromatic, polar, and basic C-terminal residues. No activity with N-carboxybenzoyl-Ala-Pro, N-carboxybenzoyl-Ala-Asp, N-carboxybenzoyl-Ala-Gly, N-carboxybenzoyl-Ala-Glu.
show the reaction diagram
-
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro + L-phenylalanine
show the reaction diagram
-
-
-
?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His + H2O
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe + L-histidine
show the reaction diagram
-
-
-
?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + H2O
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His + L-leucine
show the reaction diagram
-
-
-
?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu + H2O
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu + L-leucine
show the reaction diagram
-
-
-
?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val + H2O
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu + L-valine
show the reaction diagram
-
-
-
?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr + H2O
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val + L-tyrosine
show the reaction diagram
-
-
-
?
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr-Ser + H2O
Ac-Asp-Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu-Leu-Val-Tyr + L-serine
show the reaction diagram
stepwise hydrolysis of up to seven residues from the C-terminus
-
-
?
N-carboxybenzoyl-Ala-Ala + H2O
N-carboxybenzoyl-Ala + L-alanine
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Arg + H2O
N-carboxybenzoyl-Ala + L-arginine
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Ile + H2O
N-carboxybenzoyl-Ala + L-isoleucine
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Leu + H2O
N-carboxybenzoyl-Ala + L-leucine
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Lys + H2O
N-carboxybenzoyl-Ala + L-lysine
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Met + H2O
N-carboxybenzoyl-Ala + L-methionine
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Phe + H2O
N-carboxybenzoyl-Ala + L-phenylalanine
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Trp + H2O
N-carboxybenzoyl-Ala + L-tryptophan
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Tyr + H2O
N-carboxybenzoyl-Ala + L-tyrosine
show the reaction diagram
-
-
-
?
N-carboxybenzoyl-Ala-Val + H2O
N-carboxybenzoyl-Ala + L-valine
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
purified in its inactive state by the addition of EDTA and dithiothreitol to purification buffers, and the activity is restored by the addition of Co2+. No activation by Zn2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTT
2 mM, complete inhibition
EDTA
1 mM, complete inhibition. Inhibition is fully reversible upon 50fold dilution and re-activation by Co2+
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
N-carboxybenzoyl-Ala-Arg
80°C, pH 6.5, 0.4 mM CoCl2
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
600
N-carboxybenzoyl-Ala-Arg
Pyrococcus furiosus
Q8U3L0
80°C, pH 6.5, 0.4 mM CoCl2
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
667
N-carboxybenzoyl-Ala-Arg
Pyrococcus furiosus
Q8U3L0
80°C, pH 6.5, 0.4 mM CoCl2
42212
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1391
substrate: N-carboxybenzoyl-Ala-Arg, 80°C, pH 6.5, 0.4 mM CoCl2
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3 - 7.3
pH 5.3: about 50% of maximal activity, pH 7.3: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
58000
2 * 58000, SDS-PAGE
59000
2 * 59000, matrix-assisted laser desorption ionization time-of flight mass spectrometry
128000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
2 * 58000, SDS-PAGE; 2 * 59000, matrix-assisted laser desorption ionization time-of flight mass spectrometry
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
the overall structure of the holoenzyme is extremely thermostable. The activities of both the apo and holo enzyme exhibit a similar second-order decay over time, with 50% activity remaining after 40 min at 80°C
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
purified in its inactive state by the addition of EDTA and dithiothreitol to purification buffers, and the activity is restored by the addition of Co2+