Information on EC 3.4.17.6 - alanine carboxypeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.17.6
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RECOMMENDED NAME
GeneOntology No.
alanine carboxypeptidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Release of a C-terminal alanine from a peptide or a variety of pteroyl or acyl groups
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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exopeptidase, C-terminus, amino acid
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-70-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
mutant K12 with decreased activity
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
Soil bacterium
unidentified
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-amino-4-deoxypteroyl-Ala + H2O
4-amino-4-deoxypteroate + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
4-aminobenzoyl-Ala + H2O
4-aminobenzoate + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
4-aminobenzoylalanine + H2O
?
show the reaction diagram
Soil bacterium
-
-
-
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acyl-Ala + H2O
? + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
Ala-Ala + H2O
Ala + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
benzoyl-Ala + H2O
benzoate + Ala
show the reaction diagram
benzyloxycarbonyl-Ala + H2O
benzyloxy-CHO + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
benzyloxycarbonyl-Ala-Ala + H2O
benzyloxycarbonyl-Ala + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
benzyloxycarbonyl-Asp-Ala + H2O
benzyloxycarbonyl-Asp + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
benzyloxycarbonyl-Ile-Ala + H2O
benzyloxycarbonyl-Ile + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
benzyloxycarbonyl-Leu-Ala + H2O
benzyloxycarbonyl-Leu + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
benzyloxycarbonyl-Met-Ala + H2O
benzyloxycarbonyl-Met + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
benzyloxycarbonyl-Phe-Ala + H2O
benzyloxycarbonyl-Phe + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
benzyloxycarbonyl-Ser-Ala + H2O
benzyloxycarbonyl-Ser + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
Glu-Ala + H2O
Glu + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
Ile-Ala + H2O
Ile + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
Leu-Ala + H2O
Leu + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
Met-Ala + H2O
Met + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
N-benzoyl-Gly-Ala + H2O
N-benzoyl-Gly + Ala
show the reaction diagram
N-benzoyl-L-aminobutyric acid + H2O
benzoate + L-aminobutyric acid
show the reaction diagram
p-chlorobenzoyl-Ala + H2O
p-chlorobenzoate + Ala
show the reaction diagram
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-
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ir
p-chlorobenzoyl-Gly + H2O
p-chlorobenzoate + Gly
show the reaction diagram
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-
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ir
p-hydroxybenzoyl-Ala + H2O
p-hydroxybenzoate + Ala
show the reaction diagram
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-
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ir
p-methoxybenzoyl-Gly + H2O
p-methoxybenzoate + Gly
show the reaction diagram
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-
-
ir
p-methoxyenzoyl-Ala + H2O
p-methoxybenzoate + Ala
show the reaction diagram
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-
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ir
p-nitrobenzoyl-Ala + H2O
p-nitrobenzoate + Ala
show the reaction diagram
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-
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ir
p-nitrobenzoyl-Gly + H2O
p-nitrobenzoate + Gly
show the reaction diagram
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-
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ir
peptidyl-L-alanine + H2O
?
show the reaction diagram
Soil bacterium
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peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position
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Phe-Ala + H2O
Phe + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
Ser-Ala + H2O
Ser + Ala
show the reaction diagram
Soil bacterium
-
-
-
ir
Tyr-Ala + H2O
Tyr + Ala
show the reaction diagram
Soil bacterium
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-
-
ir
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-aminobenzoylalanine + H2O
?
show the reaction diagram
Soil bacterium
-
-
-
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peptidyl-L-alanine + H2O
?
show the reaction diagram
Soil bacterium
-
peptide can be replaced by a variety of pteroyl or acyl groups, absolute specificity for alanine in carboxyl-terminal position
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-
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
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146% activation at 0.5 mM, optimum concentration
Mg2+
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can partially replace cobalt
Ni2+
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can partially replace cobalt
Zn2+
Soil bacterium
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required
additional information
Soil bacterium
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no effect: Co2+, Mn2+, Mg2+, Fe3+, Fe2+, Cu2+, Cd2+, Ni2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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100% inhibition at 1 mM
2-2'-dipyridyl
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80% inhibition at 1 mM
8-hydroxyquinoline
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100% inhibition at 1 mM
Ag2+
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100% inhibition at 1 mM
Benzoyl-Ala
Soil bacterium
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at least 30% inhibition at 0.3 mM; competitive, Ki: 0.009 mM
benzoyl-Leu-Ala
Soil bacterium
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at least 30% inhibition at 0.3 mM
carboxyl-terminal Ala containing compounds
Soil bacterium
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Cu2+
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100% inhibition at 1 mM
Fe2+
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33% inhibition at 1 mM
Fe3+
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47% inhibition at 1 mM
Gly-Ala
Soil bacterium
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non-competitive, Ki: 0.13 mM
Hg2+
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100% inhibition at 1 mM
His-Ala
Soil bacterium
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non-competitive, Ki: 0.34 mM
iodoacetic acid
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25% inhibition at 1 mM
PCMB
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100% inhibition at 1 mM
Phe-Ala
Soil bacterium
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at least 30% inhibition at 0.3 mM
Phosphate buffer
Soil bacterium
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Tyr-Ala
Soil bacterium
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at least 30% inhibition at 0.3 mM
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.3
N-Benzoyl-Ala
Soil bacterium
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6.7
N-benzoyl-Gly
Soil bacterium
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4.3
N-benzoyl-L-aminobutyric acid
Soil bacterium
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.009
Benzoyl-Ala
Soil bacterium
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0.13
Gly-Ala
Soil bacterium
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non-competitive
0.34
His-Ala
Soil bacterium
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non-competitive
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.3
Soil bacterium
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additional information
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activities per g wet weight 10fold higher in wild-type than in K12 cells
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 8
Soil bacterium
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
Soil bacterium
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
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6 * 40000, SDS-PAGE
230000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
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6 * 40000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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below
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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possible penicillin target