Information on EC 3.4.17.19 - Carboxypeptidase Taq

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.17.19
-
RECOMMENDED NAME
GeneOntology No.
Carboxypeptidase Taq
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Release of a C-terminal amino acid with broad specificity, except for -Pro
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9031-98-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Carbobenzoxy-Gly-Ala + H2O
Carbobenzoxy-Gly + Ala
show the reaction diagram
Carbobenzoxy-Gly-Leu + H2O
Carbobenzoxy-Gly + Leu
show the reaction diagram
Carbobenzoxy-Gly-Phe + H2O
Carbobenzoxy-Gly + Phe
show the reaction diagram
Carbobenzoxy-Gly-Pro-Leu-Gly + H2O
Carbobenzoxy-Gly-Pro-Leu + Gly
show the reaction diagram
-
-
-
-
-
Carbobenzoxy-Gly-Val + H2O
Carbobenzoxy-Gly + Val
show the reaction diagram
Carbobenzoxy-Phe-Tyr + H2O
Carbobenzoxy-Phe + Tyr
show the reaction diagram
N-(3-[2-furyl]acryloyl)-Ala-Lys + H2O
?
show the reaction diagram
-
-
-
?
N-(3-[2-furyl]acryloyl)-Phe-Phe + H2O
?
show the reaction diagram
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
50% of the activation with Co2+
Co2+
-
dependent on
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
Ca2+
at 1 mM 92.2% activity relative to control
Co2+
at 0.01 mM 59.7% activity relative to control
guanidine hydrochloride
-
-
Metal chelating reagents
-
-
-
Mg2+
at 1 mM 88% activity relative to control
Mn2+
at 0.1 mM 76% activity relative to control
Ni2+
at 0.01 mM 44.6% activity relative to control
o-phenanthroline
1 mM strongly inhibits
Zn2+
at 0.01 mM 8.98% activity relative to control
additional information
PMSF at 2 mM and E-64 at 0.001 mM have no effect on activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.166 - 0.169
N-(3-[2-furyl]acryloyl)-Ala-Lys
0.073
N-(3-[2-furyl]acryloyl)-Phe-Phe
in the presence of Co2+
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.7 - 20
N-(3-[2-furyl]acryloyl)-Ala-Lys
0.7
N-(3-[2-furyl]acryloyl)-Phe-Phe
Trypanosoma cruzi
Q6ZXC0
in the presence of Co2+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2
cell-free extract
85
20.4fold purified enzyme
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.5
-
pH 6: about 35% of activity maximum, pH 8.5: about 85% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 90
-
30°C: less than 30% of activity maximum, 90°C: more than 90% of activity maximum
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
cell-derived and metacylic trypomastigotes
Manually annotated by BRENDA team
additional information
present in all life cycle stages
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Thermus thermophilus (strain HB27 / ATCC BAA-163 / DSM 7039)
Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
-
1 * 56000, Thermus aquaticus, SDS-PAGE
56210
-
Thermus aquaticus, calculation from nucleotide sequence
57700
x * 57700, sequence analysis, x * 59000, Western blot analysis
58000
-
Thermus aquaticus, gel filtration
59000
x * 57700, sequence analysis, x * 59000, Western blot analysis
128000
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 57700, sequence analysis, x * 59000, Western blot analysis
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 10
-
4°C, 18 h, stable
28905
7 - 9
-
25°C, 5 h, stable
28905
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
-
pH 7-10, 18 h, stable
25
-
pH 7-9, 5 h, stable
80
-
5 h, 10% loss of activity
90
-
5 h, about 80% loss of activity
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gel filtration and two ion-exchange chromatography steps (Ni- and Co-NTA resin), 20.4fold purification and 32% recovery
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli XL1 Blue cells as catalytically active poly-His-tagged recombinant enzyme. GST-fusion proteins expressed in Escherichia coli BL21 Codon Plus (DE3) cells
expression in Escherichia coli
-
expression in Pichia pastoris GS115
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
potential target for drug development
synthesis
expression of enzyme gene optimized for the Pichia pastoris codon usage and secretory expression. System is applicable for large-scale preparation of enzyme
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