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Information on EC 3.4.17.10 - carboxypeptidase E and Organism(s) Homo sapiens and UniProt Accession P16870

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.17 Metallocarboxypeptidases
                3.4.17.10 carboxypeptidase E
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This record set is specific for:
Homo sapiens
UNIPROT: P16870 not found.
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The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
release of C-terminal arginine or lysine residues from polypeptides
Synonyms
cpe, carboxypeptidase e, carboxypeptidase h, metallocarboxypeptidase, enkephalin convertase, membrane-bound carboxypeptidase, egl-21, carboxypeptidase e/h, prohormone processing carboxypeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carboxypeptidase E
-
CPE-NFalpha1
-
neurotrophic factor-alpha1
-
carboxypeptidase E
carboxypeptidase, enkephalin precursor
-
-
-
-
cobalt stimulated chromaffin granule carboxypeptidase
-
-
-
-
CPH
-
-
-
-
enkephalin convertase
-
-
-
-
enkephalin precursor carboxypeptidase
-
-
-
-
enkephalin-precursor endopeptidase
-
-
-
-
hCPE
-
-
insulin granule-associated carboxypeptidase
-
-
-
-
membrane-bound carboxypeptidase
-
-
-
-
peptidyl-L-lysine(-L-arginine) hydrolase
-
-
-
-
Prohormone processing carboxypeptidase
-
-
-
-
additional information
-
the enzyme belongs to the family of metallocarboxypeptidases
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
81876-95-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-Tyr-Ala-Arg + H2O
acetyl-Tyr-Ala + Arg
show the reaction diagram
-
-
-
-
?
benzoyl-Phe-Ala-Arg + H2O
benzoyl-Phe-Ala + Arg
show the reaction diagram
-
-
-
-
?
Dansyl-Phe-Ala-Arg + H2O
Dansyl-Phe-Ala + Arg
show the reaction diagram
-
-
-
-
?
furylacrylic-Ala-Arg + H2O
furylacrylic-Ala + Arg
show the reaction diagram
-
-
-
-
?
[Leu5]enkephalin-Arg6-Arg7 + H2O
[Leu5]enkephalin + Arg
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zn2+
-
metalloenzyme
additional information
-
metallocarboxypeptidase
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
-
-
EDTA
-
-
Guanidinoethylmercaptosuccinic acid
-
-
HgCl2
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 5.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
CPE reaction
additional information
-
rapid decline in activity at pH values above pH 5.5
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
CPE reaction
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
about
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the enzyme colocalizes with and interacts with human growth hormone in AtT20 pituitary cells
Manually annotated by BRENDA team
-
immortalized bronchial epithelial cell line
Manually annotated by BRENDA team
-
contitioned culture medium, from hepatoma cells
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
primary, 17 different biopsies
Manually annotated by BRENDA team
additional information
-
gene expression analysis in lung tumor tissue, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the enzyme is produced from pro-carboxypeptidase E and exists in soluble and membrane forms
Manually annotated by BRENDA team
the enzyme is produced from pro-carboxypeptidase E and exists in soluble and membrane forms
-
Manually annotated by BRENDA team
-
secretion from neuroendocrine tissue
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
in two types of carboxypeptidase E mutant mice, Cpefat/Cpefat and Cpe knockout, loss of normal carboxypeptidase E leads to a lot of disorders, including diabetes, hyperproinsulinemia, low bone mineral density and deficits in learning and memory
physiological function
malfunction
-
in Neuro 2a cells that lack CPE, PC1/3 and PC2, proglucagon co-localise with the Golgi marker p115 as determined by quantitative immunofluorescence microscopy. siRNA-mediated knockdown of CPE disrupts regulated secretion of glucagon from pancreatic-derived alphaTC1-6 cells, but not of GLP-1 from intestinal cell-derived GLUTag cells
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CBPE_HUMAN
476
0
53151
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52000 - 54000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
structure overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
glycosylation does not appear to affect the enzyme activity
side-chain modification
-
glycoprotein
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R283W
-
naturally occurring genetic variant of the enzyme, the mutant shows altered kinetics, heat stability, and pH optimum, and a 50% reduced activity compared to the wild-type enzyme
additional information
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, partially purified enzyme, 1 month, 90% loss of activity
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ion exchange, heparin affinity and gel filtration chromatography
-
partial
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CPE gene and total gene quantitative expression analysis in lung tumor tissue, detailed overview
-
DNA and amino acid sequence determination, localization on chromosome 4, several naturally occurring genetic variants exist
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expression in HEK-293 cells
-
interaction of the recombinant enzyme with recombinnat eosinophil cationic protein in the yeast two-hybrid system via residues 318-387 of CPE, overview, expression of the enzyme fused to the activation domain of Gal4 in GH3 cells
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
medicine
biotechnology
-
carboxypeptidase E is used in an in vitro system for the production of bioactive peptides
diagnostics
-
CPE serves as a marker for pulmonary endocrine tumor cells in the lung, enzyme expression correlates with a good prognosis, while expression of gamma-glutamyl hydrolase is correlated with a poor prognosis, overview
drug development
-
carboxypeptidase E plays a role in the regulation of dopamine transporter trafficking and dopamine transporter-mediated dopamine uptake, which may provide a novel target in the treatment of dopamine-governed diseases such as drug addiction and obesity
medicine
-
the polymorphism 801 C to T in the carboxypeptidase E exon5 gene may contribiute to the angiographical characteristics of coronary atherosclerosis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Grimwood, B.G.; Plummer, T.H.; Tarentino, A.L.
Carboxypeptidase H. A regulatory peptide-processing enzyme produced by human hepatoma Hep G2 cells
J. Biol. Chem.
264
15662-15667
1989
Homo sapiens
Manually annotated by BRENDA team
Hook, V.Y.H.; Affolter, H.U.
Identification of zymogen and mature forms of human carboxypeptidase H. A processing enzyme for the synthesis of peptide hormones
FEBS Lett.
238
338-342
1988
Homo sapiens
Manually annotated by BRENDA team
Deddish, P.A.; Skidgel, R.A.; Erds, E.G.
Enhanced Co2+ activation and inhibitor binding of carboxypeptidase M at low pH. Similarity to carboxypeptidase H (enkephalin convertase)
Biochem. J.
261
289-291
1989
Homo sapiens
Manually annotated by BRENDA team
Manser, E.; Fernandez, D.; Loo, L.; Goh, P.Y.; Monfries, C.; Hall, C.; Lim, L.
Human carboxypeptidase E. Isolation and characterization of the cDNA, sequence conservation, expression and processing in vitro
Biochem. J.
267
517-525
1990
Homo sapiens
Manually annotated by BRENDA team
Wu, C.M.; Chang, H.T.; Chang, M.D.
Membrane-bound carboxypeptidase E facilitates the entry of eosinophil cationic protein into neuroendocrine cells
Biochem. J.
382
841-848
2004
Homo sapiens
Manually annotated by BRENDA team
Fricker, L.D.
Carboxypeptidase E
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. ) Academic Press
2
840-844
2004
Aplysia sp., Bos taurus, Homo sapiens, Mus musculus, Rattus norvegicus
-
Manually annotated by BRENDA team
He, P.; Varticovski, L.; Bowman, E.D.; Fukuoka, J.; Welsh, J.A.; Miura, K.; Jen, J.; Gabrielson, E.; Brambilla, E.; Travis, W.D.; Harris, C.C.
Identification of carboxypeptidase E and gamma-glutamyl hydrolase as biomarkers for pulmonary neuroendocrine tumors by cDNA microarray
Hum. Pathol.
35
1196-1209
2004
Homo sapiens
Manually annotated by BRENDA team
Ozawa, A.; Cai, Y.; Lindberg, I.
Production of bioactive peptides in an in vitro system
Anal. Biochem.
366
182-189
2007
Homo sapiens
Manually annotated by BRENDA team
Wang, J.; Zhang, Y.; Yang, Z.J.; Zhu, T.B.; Wang, L.S.; Chen, B.; Cao, K.J.; Huang, J.; Ma, W.Z.; Jia, E.Z.
Association of human carboxypeptidase E exon5 gene polymorphisms with angiographical characteristics of coronary atherosclerosis in a Chinese population
Acta Pharmacol. Sin.
29
736-744
2008
Homo sapiens
Manually annotated by BRENDA team
Oiso, S.; Takeda, Y.; Futagawa, T.; Miura, T.; Kuchiiwa, S.; Nishida, K.; Ikeda, R.; Kariyazono, H.; Watanabe, K.; Yamada, K.
Contactin-associated protein (Caspr) 2 interacts with carboxypeptidase E in the CNS
J. Neurochem.
109
158-167
2009
Homo sapiens
Manually annotated by BRENDA team
Jia, E.; Wang, J.; Yang, Z.; Zhu, T.; Wang, L.; Wang, H.; Li, C.; Chen, B.; Cao, K.; Huang, J.; Ma, W.
Association of the mutation for the human carboxypeptidase e gene exon 4 with the severity of coronary artery atherosclerosis
Mol. Biol. Rep.
36
245-254
2009
Homo sapiens (P16870), Homo sapiens
Manually annotated by BRENDA team
Zhang, H.; Li, S.; Wang, M.; Vukusic, B.; Pristupa, Z.B.; Liu, F.
Regulation of dopamine transporter activity by carboxypeptidase E
Mol. Brain
2
10
2009
Homo sapiens, synthetic construct
Manually annotated by BRENDA team
Kumar, S.; Tomar, A.K.; Singh, S.; Gill, K.; Dey, S.; Singh, S.; Yadav, S.
Heparin binding carboxypeptidase E protein exhibits antibacterial activity in human semen
Int. J. Biol. Macromol.
64
319-327
2014
Homo sapiens
Manually annotated by BRENDA team
McGirr, R.; Guizzetti, L.; Dhanvantari, S.
The sorting of proglucagon to secretory granules is mediated by carboxypeptidase E and intrinsic sorting signals
J. Endocrinol.
217
229-240
2013
Homo sapiens
Manually annotated by BRENDA team
Ji, L.; Wu, H.T.; Qin, X.Y.; Lan, R.
Dissecting carboxypeptidase E properties, functions and pathophysiological roles in disease
Endocr. Connect.
6
R18-R38
2017
Homo sapiens (P16870), Mus musculus (Q00493)
Manually annotated by BRENDA team
Xiao, L.; Yang, X.; Loh, Y.P.
Neurotrophic, gene regulation, and cognitive functions of carboxypeptidase E-neurotrophic factor-alpha1 and its variants
Front. Neurosci.
13
243
2019
Homo sapiens (P16870), Mus musculus (Q00493)
Manually annotated by BRENDA team
Mizutani, A.; Inoko, H.; Tanaka, M.
Carboxypeptidase E, identified as a direct interactor of growth hormone, is important for efficient secretion of the hormone
Mol. Cells
39
756-761
2016
Homo sapiens (P16870), Homo sapiens
Manually annotated by BRENDA team
Sun, J.; Meng, D.; Li, L.; Tian, X.; Jia, Y.; Wang, H.; Yu, H.; Sun, T.; Qu, A.; Shen, H.; Bao, J.; Zhang, G.
N-terminal truncated carboxypeptidase E expression is associated with poor prognosis of lung adenocarcinoma
Oncol. Lett.
12
4659-4664
2016
Homo sapiens (P16870), Homo sapiens
Manually annotated by BRENDA team
Huang, S.F.; Wu, H.D.; Chen, Y.T.; Murthy, S.R.; Chiu, Y.T.; Chang, Y.; Chang, I.C.; Yang, X.; Loh, Y.P.
Carboxypeptidase E is a prediction marker for tumor recurrence in early-stage hepatocellular carcinoma
Tumour Biol.
37
9745-9753
2016
Homo sapiens (P16870), Homo sapiens
Manually annotated by BRENDA team