Information on EC 3.4.17.10 - carboxypeptidase E

Word Map on EC 3.4.17.10
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.17.10
-
RECOMMENDED NAME
GeneOntology No.
carboxypeptidase E
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of C-terminal arginine or lysine residues from polypeptides
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
81876-95-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
dolphin
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
equine herpesvirus type 2
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
human adenovirus
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
Mus musculus C57BL/6
C57/BL6 mice
-
-
Manually annotated by BRENDA team
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
pheasant
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
plant
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
1400051 A+, 1400174 A+, 1444897 A+, 1444909 A+, 1444971 A+, 1445130 A+, 1445431 A+, 1445439 A+, 1445454 A+, 1445501 A+, 1445545 A+, 1445659 A+, 1445665 A+, 1445871 A+, 1445879 A+, 2375724 A+, 2571052 A+, 2571133 A+, 2571230 A+, 2571295 A+, 2571313 A+, 2571346 A+, 2571394 A+, 732743 A+, 776309 A+, 776498 A+, 81307 A+, 822365 A+, 822368 A+, 845884 A+, 845906 A+, 1285137 A++, 1285179 A++, 1400159 A++, 1400182 A++, 1437660 A++, 1437823 A++, 1444929 A++, 1444948 A++, 1444957 A++, 1445108 A++, 1445140 A++, 1445292 A++, 1445309 A++, 2147970 A++, 2163702 A++, 2163705 A++, 2163706 A++, 2163717 A++, 2430798 A++, 2532252 A++, 2570990 A++, 2571142 A++, 683068 A++, 709596 A++, 81293 A++, 81294 A++, 81296 A++, 81297 A++, 822303 A++, 845810 A++, 845849 A++, 845874 A++, 1285141 A++, 1297565 A++, 1400119 A++, 1401354 A++, 1444925 A++, 1445136 A++, 1445142 A++, 1445153 A++, 1445201 A++, 1445311 A++, 2163699 A++, 2163700 A++, 2163703 A++, 2163707 A++, 2163708 A++, 2163711 A++, 2163712 A++, 2163713 A++, 2163714 A++, 2163716 A++, 2163719 A++, 2163720 A++, 2163722 A++, 2163751 A++, 2550647 A++, 2571019 A++, 2571144 A++, 28969 A++, 669606 A++, 683932 A++, 760173 A++, 810539 A++, 81295 A++, 81304 A++, 81305 A++, 821426 A++, 821495 A++, 821514 A++, 843759 A++, 845803 A++, 845804 A++, 845805 A++, 845809 A++, 845811 A++, 845819 A++, 845832 A++, 845833 A++, 845834 A++, 845841 A++, 845859 A++, 845882 A++, 845903 A++, 845851 A+++, 668863 A++++, 842363 A++++, 845801 A++++, 845802 A++++, 845812 A++++, 845815 A++++, 845816 A++++, 845817 A++++, 845818 A++++, 845820 A++++, 845822 A++++, 845823 A++++, 845825 A++++, 845827 A++++, 845830 A++++, 845831 A++++, 845835 A++++, 845838 A++++, 845839 A++++, 845840 A++++, 845858 A++++, 845867 A++++
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
salmon
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
shark
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
synthetic construct
-
-
-
Manually annotated by BRENDA team
toad
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
-
-
-
Automatic Mining of ENzyme DAta
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
-
the amino acid sequence of carboxypeptidase E appears to be highly conserved among chicken, mouse, and human. The C-terminal sequence of carboxypeptidase E, which serves as an anchor to the membrane of secretory granules, is perfectly conserved between birds and mammals
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(Met)enkephalin-Arg + H2O
Tyr-Gly-Gly-Phe-Met + Arg
show the reaction diagram
(Met)enkephalin-Lys + H2O
Tyr-Gly-Gly-Phe-Met + Lys
show the reaction diagram
acetyl-Tyr-Ala-Arg + H2O
acetyl-Tyr-Ala + Arg
show the reaction diagram
benzoyl-Phe-Ala-Arg + H2O
benzoyl-Phe-Ala + Arg
show the reaction diagram
D-Tyr-Ala-His-Lys-Lys + H2O
D-Tyr-Ala-His-Lys + Lys
show the reaction diagram
-
-
-
-
?
Dansyl-Ala-Arg + H2O
Dansyl-Ala + Arg
show the reaction diagram
-
-
-
-
?
dansyl-Gly-Lys + H2O
dansyl-Gly + Lys
show the reaction diagram
-
-
-
-
?
Dansyl-Phe-Ala-Arg + H2O
Dansyl-Phe-Ala + Arg
show the reaction diagram
Dansyl-Phe-Gly-Arg + H2O
Dansyl-Phe-Gly + Arg
show the reaction diagram
-
-
-
-
?
Dansyl-Phe-Ile-Arg + H2O
Dansyl-Phe-Ile + Arg
show the reaction diagram
-
-
-
-
?
dansyl-Phe-Leu-Ala-Arg + H2O
dansyl-Phe-Leu-Ala + Arg
show the reaction diagram
-
-
-
-
?
Dansyl-Phe-Leu-Arg + H2O
Dansyl-Phe-Leu + Arg
show the reaction diagram
dansyl-Phe-Leu-Gly-Arg + H2O
dansyl-Phe-Leu-Gly + Arg
show the reaction diagram
-
-
-
-
?
Dansyl-Pro-Ala-Arg + H2O
Dansyl-Pro-Ala + Arg
show the reaction diagram
-
-
-
-
?
enkephalin precursor + H2O
?
show the reaction diagram
-
-
-
-
?
furylacrylic-Ala-Arg + H2O
furylacrylic-Ala + Arg
show the reaction diagram
-
-
-
-
?
hippuryl-Arg + H2O
hippuric acid + Arg
show the reaction diagram
-
-
-
-
?
iodo-acetyl-Tyr-Ala-Arg + H2O
iodo-acetyl-Tyr-Ala + Arg
show the reaction diagram
-
-
-
-
?
pro-islet amyloid polypeptide + H2O
islet amyloid polypeptide + pro-islet amyloid polypeptide propeptide
show the reaction diagram
proinsulin tryptic peptides + H2O
?
show the reaction diagram
-
-
-
-
?
[Leu5]enkephalin-Arg6-Arg7 + H2O
[Leu5]enkephalin + Arg
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pro-islet amyloid polypeptide + H2O
islet amyloid polypeptide + pro-islet amyloid polypeptide propeptide
show the reaction diagram
-
processing in pancreatic beta-cells
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
enhances the binding of CPE to secretogranin III
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
2-mercaptomethyl-3-guanidinoethylthiopropanoic acid
-
-
4-Chloromercuriphenylsulfonate
-
over 95% inhibition at 0.1 mM
Aminopropylmercapto succinic acid
arginine
-
-
CdCl2
CuCl2
Guanidinoethylmercaptosuccinic acid
HgCl2
Leu-enkephalin
Luteinizing hormone-releasing hormone
lysine
-
-
Met-enkephalin
Met-Enkephalin-Arg-Gly-Leu
Met-Enkephalin-Arg6-Phe7
Oxytoxin
p-Chloromercuriphenyl sulfonate
Substance P
sulfhydryl reagents
thyrotropin-releasing hormone
Vasopressin
[Leu5]Enkephalin-Arg6
-
-
[Leu5]Enkephalin-Lys6
-
-
[Met5]Enkephalin-Arg6
-
-
[Met5]Enkephalin-Lys6
-
-
additional information
-
excessive secretogranin III leads to a decrease in CPE-bound proopiomelanocortin-derived intermediate molecules
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
allyl isothiocyanate
-
mRNA for carboxypeptidase E is transiently increased in the first 6 h, after which is decreased to below baseline levels, after intracolonically administration of allyl isothiocyanate
additional information
-
tryptic activation of the pancreatic zymogen
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.1 - 0.12
Dansyl-Ala-Arg
-
-
0.018 - 0.034
dansyl-Phe-Ala-Arg
0.029 - 0.031
dansyl-Phe-Gly-Arg
-
-
0.068 - 0.077
dansyl-Phe-Ile-Arg
-
-
0.34 - 0.49
dansyl-Phe-Leu-Ala-Arg
-
-
0.0175 - 0.071
dansyl-Phe-Leu-Arg
0.16 - 0.18
dansyl-Phe-Leu-Gly-Arg
-
-
0.058 - 0.062
Dansyl-Pro-Ala-Arg
-
-
0.4
hippuryl-Arg
-
-
0.028
iodo-acetyl-Tyr-Ala-Arg
-
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
13
dansyl-Phe-Ala-Arg
Sus scrofa
-
-
8.5
hippuryl-Arg
Sus scrofa
-
-
16
iodo-acetyl-Tyr-Ala-Arg
Sus scrofa
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
metabolic parameters of wild-type, heterozygous and homozygeous enzyme-deficient mutant mice, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 5.5
5 - 5.5
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6.5
-
pH 4: about 20% of activity maximum, pH 6.5: about 15% of activity maximum
5 - 6
-
-
5
-
CPE reaction
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1
-
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
immortalized bronchial epithelial cell line
Manually annotated by BRENDA team
-
-
Automatic Mining of ENzyme DAta
-
contitioned culture medium, from hepatoma cells
Manually annotated by BRENDA team
-
transiently expressed in mouse embryos from embryonic day 5.5 to postnatal day 1
Manually annotated by BRENDA team
-
specifically expressed in enteroendocrine cell of the murine colon and ileum
Manually annotated by BRENDA team
-
CPE expression occurs in gastrin synthesizing cells and in gastrin-somatostatin progenitor cells
Manually annotated by BRENDA team
-
present in some dendrites and nerve terminals, which contain storage vesicles
Manually annotated by BRENDA team
-
primary, 17 different biopsies
Manually annotated by BRENDA team
additional information