Information on EC 3.4.16.4 - serine-type D-Ala-D-Ala carboxypeptidase

New: Word Map on EC 3.4.16.4
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Search Reference ID:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
3.4.16.4
-
RECOMMENDED NAME
GeneOntology No.
serine-type D-Ala-D-Ala carboxypeptidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferential cleavage: (Ac)2-L-Lys-D-Ala-/-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of amide bond
-
hydrolysis of peptide bond
transpeptidation
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
peptidoglycan biosynthesis
-
-
Peptidoglycan biosynthesis
-
-
peptidoglycan biosynthesis II (staphylococci)
-
-
peptidoglycan biosynthesis IV (Enterococcus faecium)
-
-
peptidoglycan maturation (meso-diaminopimelate containing)
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9077-67-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
544 Brucella abortus biovar 1 strain and strain K23
-
-
Manually annotated by BRENDA team
BM 4147
-
-
Manually annotated by BRENDA team
BM4339
-
-
Manually annotated by BRENDA team
ATCC 9790
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Nonomuraea sp.
-
UniProt
Manually annotated by BRENDA team
Ochrobactrum anthropi SV3
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Streptomyces pneumoniae
-
-
-
Manually annotated by BRENDA team
K11
-
-
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
Vibrio cholerae ATCC 39315
-
UniProt
Manually annotated by BRENDA team
KP+, serotype O3:K6
UniProt
Manually annotated by BRENDA team
KP+, serotype O3:K6
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
loss of penicillin-binding protein 5 enhances beta-lactam susceptibility, the observed susceptibilities for ampicillin, piperacillin, amoxicillin, penicillin G, cefadroxil and cefalexin are enhanced by 4fold and for cefalothin and cefaclor the susceptibilities are at least 8fold higher in the mutants
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(2R)-2-[[N-(phenylacetyl)glycyl]sulfanyl]propanoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
(R)-[2-(benzoylamino)propionylsulfanyl]acetic acid + H2O
?
show the reaction diagram
-
-
-
-
?
2-(N-glycyl-L-cysteinyl)acetyl-D-alanyl-D-alanine + H2O
2-(N-glycyl-L-cysteinyl)acetyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
-
?
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
-
?
3-(N-glycyl-cysteaminyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-cysteaminyl)propanoyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
-
?
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate + H2O
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanine + D-thiolactate
show the reaction diagram
-
very efficient substrate for R61 DD-peptidase
-
-
?
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
-
?
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine + H2O
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
-
?
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate + H2O
(2R)-N-(phenylacetyl)-2-hydroxyglycine + 3-hydroxybenzoate
show the reaction diagram
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
(2R)-N-(phenylacetyl)-2-methoxyglycine + 3-hydroxybenzoate
show the reaction diagram
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate + H2O
?
show the reaction diagram
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate + H2O
?
show the reaction diagram
3-[[N-(phenylacetyl)-D-alanyl]oxy]benzoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
3-[[N-(phenylacetyl)glycyl]oxy]benzoic acid + H2O
?
show the reaction diagram
-
-
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Ala
?
show the reaction diagram
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Gln
?
show the reaction diagram
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Gln + Gly-L-Ala
Ac2-L-Lys-D-Ala-Gly-L-Ala + ?
show the reaction diagram
-
transpeptidation
-
-
r
Ac2-L-Lys-D-Ala-Gly-L-Leu
?
show the reaction diagram
-
poor substrate, but hydrolysis was significant
-
-
?
Ac2-L-Lys-D-Ala-Gly-L-Leu + D-alanine
Ac2-L-Lys-D-Ala-D-Ala + ?
show the reaction diagram
-
transpeptidation
-
-
r
acetyl-L-Lys-D-Ala-D-Ala + H2O
acetyl-L-Lys-D-Ala + D-Ala
show the reaction diagram
benzoyl-D-Ala-thioglycolate + H2O
?
show the reaction diagram
-
-
?
benzoyl-Gly-thioglycolate + H2O
?
show the reaction diagram
-
-
?
benzoyl-Gly-thiolactate + H2O
?
show the reaction diagram
-
-
?
Boc-gamma-D-Glu-L-Lys-(Cbz)-D-Ala-D-Ala
?
show the reaction diagram
-
-
-
?
bocillin FL
?
show the reaction diagram
-
-
-
-
?
D-Ala-D-Ala + H2O
D-Ala + D-Ala
show the reaction diagram
D-Ala-D-Phe + H2O
D-Ala + D-Phe
show the reaction diagram
-
-
-
-
?
D-Ala-D-Trp + H2O
D-Ala + D-Trp
show the reaction diagram
-
-
-
-
?
D-Ala-D-Tyr + H2O
D-Ala + D-Tyr
show the reaction diagram
-
-
-
-
?
D-amino acid amide + H2O
D-amino acid + ammonia
show the reaction diagram
-
-
-
?
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala-D-Ala + H2O
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala + D-Ala
show the reaction diagram
-
-
-
-
?
Gly-gamma-L-Glu-D-Ala-D-Ala + H2O
Gly-gamma-L-Glu-D-Ala + D-Ala
show the reaction diagram
-
-
-
?
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala-D-Ala + H2O
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala + D-Ala
show the reaction diagram
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-alanine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-leucine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-leucine
show the reaction diagram
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-D-norleucine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + D-norleucine
show the reaction diagram
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycine + H2O
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanine + glycine
show the reaction diagram
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-alanine + H2O
?
show the reaction diagram
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-leucine + H2O
?
show the reaction diagram
-
-
-
-
?
glycyl-L-alpha-amino-epsilon-pimelyl-D-alanyl-glycyl-L-phenylalanine + H2O
?
show the reaction diagram
-
-
-
-
?
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]amino]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine + H2O
?
show the reaction diagram
-
-
-
-
?
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine + H2O
?
show the reaction diagram
-
-
-
-
?
hippuryl-mercaptoacetic acid
hippuric acid + mercaptoacetate
show the reaction diagram
-
-
-
-
?
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O
?
show the reaction diagram
-
only substrate of penicillin-binding protein 5, but not of penicillin-binding protein 6
-
-
?
L-Lys-D-Ala-D-Ala + H2O
L-Lys-D-Ala + D-Ala
show the reaction diagram
-
-
-
-
?
N,N'-diacetyl-L-Lys-D-Ala-D-Ala + H2O
?
show the reaction diagram
Streptomyces pneumoniae
-
-
-
-
?
N,N'-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
?
show the reaction diagram
-
-
-
-
?
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanine + D-Ala
show the reaction diagram
-
-
-
?
N-acetyl-D-Ala-D-Ala + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-D-Ala-D-Ala + H2O
N-acetyl-D-Ala + D-Ala
show the reaction diagram
-
-
-
-
?
N-acetyl-L-Lys-D-Ala-D-Ala + H2O
N-acetyl-L-Lys-D-Ala + D-Ala
show the reaction diagram
-
-
-
-
?
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
?
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine + H2O
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
?
N-alpha-acetyl-L-Lys-D-Ala-thiolactate + H2O
?
show the reaction diagram
-
-
?
N-benzoyl-D-Ala + H2O
benzoate + D-Ala
show the reaction diagram
-
substrate binding structure
-
-
?
N-benzoyl-D-Ala-thio-D-lactate
?
show the reaction diagram
-
-
-
?
N-benzoyl-D-Ala-thioglycolate
?
show the reaction diagram
-
-
-
?
N-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]-D-alanyl-D-alanine + H2O
?
show the reaction diagram
-
-
-
-
?
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine + H2O
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanine + D-Ala
show the reaction diagram
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + Gly-Gly
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly-Gly + D-Ala
show the reaction diagram
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
?
show the reaction diagram
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
N,N'-diacetyl-L-Lys-D-Ala + D-Ala
show the reaction diagram
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-Ala
show the reaction diagram
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-alanine
show the reaction diagram
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-lactate + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + D-lactate
show the reaction diagram
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-Gly + H2O
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala + Gly
show the reaction diagram
-
-
-
-
?
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate + H2O
?
show the reaction diagram
-
-
?
Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
?
show the reaction diagram
-
substrate in DD-carboxypeptidase activity assay
-
-
?
Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
D-alanine + Nalpha,Nepsilon-diacetyl-L-lysyl-D-alanine
show the reaction diagram
Nalpha,Nepsilon-diacetyl-Lys-D-Ala-D-Ala + H2O
Nalpha,Nepsilon-diacetyl-Lys-D-Ala + D-Ala
show the reaction diagram
-
-
-
?
Nalpha,Neta-diacetyl-L-lysyl-D-alanyl-D-alanine + H2O
?
show the reaction diagram
-
substrate for assay of DD-carboxypeptidase activity
-
-
?
Nalpha-(beta-1,4-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)-L-lysyl-D-alanyl-D-alanine + H2O
Nalpha-(beta-1,4-acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)-L-lysyl-D-alanine + D-alanine
show the reaction diagram
-
-
-
-
?
Nalpha-acetyl-L-Lys-D-Ala-D-Ala + H2O
?
show the reaction diagram
substrate is used to detect the hydrolyzing activity of PBP4a
-
-
?
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-Acetyl-L-Lys-D-Ala + D-Ala
show the reaction diagram
-
-
?
Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala-D-Ala + H2O
Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala + D-Ala
show the reaction diagram
-
-
-
?
phenylacetyl-D-Ala-thioglycolate + H2O
?
show the reaction diagram
-
-
?
phenylacetyl-D-Ala-thiolactate + H2O
?
show the reaction diagram
-
-
?
S2a + D-alanine
?
show the reaction diagram
-
-
-
-
?
S2a + D-leucine
?
show the reaction diagram
-
-
-
-
?
S2a + D-phenylalanine
?
show the reaction diagram
-
-
-
-
?
S2a + Gly-Gly
?
show the reaction diagram
-
-
-
-
?
S2a + Gly-Gly-Gly
?
show the reaction diagram
-
-
-
-
?
S2a + Gly-L-Ala
?
show the reaction diagram
-
-
-
-
?
S2a + Gly-L-Gln
?
show the reaction diagram
-
-
-
-
?
S2a + Gly-L-Leu
?
show the reaction diagram
-
one of the most efficient acceptors
-
-
?
S2d thiolester
?
show the reaction diagram
-
-
-
-
?
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
D-Ala + UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala
show the reaction diagram
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala + D-Ala
show the reaction diagram
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
show the reaction diagram
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine + H2O
UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanine + D-alanine
show the reaction diagram
X-D-Ala-D-Ala + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-Ala-D-Ala + H2O
D-Ala + D-Ala
show the reaction diagram
-
-
-
-
?
D-amino acid amide + H2O
D-amino acid + ammonia
show the reaction diagram
Q9LCC8
-
-
-
?
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
D-Ala + UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala
show the reaction diagram
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala + H2O
UDP-MurNAc-L-Ala-D-Glu-L-Lys-D-Ala + D-Ala
show the reaction diagram
UDP-N-acetyl-muramyl-L-alanyl-D-glutamyl-mesodiaminopimelyl-D-alanyl-D-alanine
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
required
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2,3)-alpha-methylene benzylpenicillin
-
modeling of enzyme-inhibitor complex
(2-phenylethyl)boronic acid
-
91% residual activity at 1 mM
(2R)-2-amino-7-oxo-7-[(4,4,4-trifluoro-3-oxobutan-2-yl)amino]heptanoic acid
-
i.e. D-alpha-aminopimelyl-(1,1,1-trifluoro-3-amino)butan-2-one
(3-aminophenyl)boronic acid
-
20% residual activity at 1 mM
(3-nitrophenyl)boronic acid
-
82% residual activity at 1 mM
(3H)benzylpenicillin
Streptomyces pneumoniae
-
-
(3R,5R)-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3R,5R,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3R,5R,6S)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3R,5S)-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3R,5S,6R)-3-benzyloxycarbonyl-6-methyl-4-dethia-4-oxa-penam
-
-
(3R,5S,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3S,5R)-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3S,5R,6S)-3-benzyloxycarbonyl-6-methyl-4-dethia-4-oxa-penam
-
-
(3S,5R,6S)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3S,5S)-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(3S,5S,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
-
-
(4-phenoxyphenyl)boronic acid
-
98% residual activity at 1 mM
(5-acetylthiophen-2-yl)boronic acid
-
88% residual activity at 1 mM
(5-chlorothiophen-2-yl)boronic acid
-
91% residual activity at 1 mM
(5-cyanothiophen-2-yl)boronic acid
-
95% residual activity at 1 mM
(5-formylthiophen-2-yl)boronic acid
-
99% residual activity at 1 mM
(5R,6R)-6-[[(6S)-6-carboxy-6-(glycylamino)hexanoyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid
-
-
(6R,7R)-3-[(acetyloxy)methyl]-7-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]amino-8-oxo-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic acid
-
-
1,2-Ethanedithiol
-
-
1,3-propanedithiol dithiothreitol
-
-
-
1-benzothiophen-2-ylboronic acid
-
86% residual activity at 1 mM
2,1-benzoxaborol-1(3H)-ol
-
45% residual activity at 1 mM
2,3-dimercapto-1-propane-sulfonic acid
-
-
2,3-dimercapto-1-propanol
-
-
2-Methylpenem
-
-
2-Phenylpenems
-
-
-
3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzoic acid
-
38% residual activity at 0.5 mM
3-(acetylamino)-5-(dihydroxyboranyl)benzoic acid
-
50% residual activity at 1 mM
3-(benzoylamino)-5-(dihydroxyboranyl)benzoic acid
-
9% residual activity at 1 mM
3-(dihydroxyboranyl)-4-[(phenylacetyl)amino]benzoic acid
-
80% residual activity at 1 mM
3-(dihydroxyboranyl)-5-[(2,6-dimethoxybenzoyl)amino]benzoic acid
-
84% residual activity at 1 mM
3-(dihydroxyboranyl)-5-[(phenoxyacetyl)amino]benzoic acid
-
2% residual activity at 1 mM
3-(dihydroxyboranyl)-5-[(thiophen-2-ylacetyl)amino]benzoic acid
-
5% residual activity at 1 mM
3-(dihydroxyboranyl)-5-[(thiophen-2-ylcarbonyl)amino]benzoic acid
-
18% residual activity at 1 mM
3-(dihydroxyboryl)-5-(2-methoxybenzamido)benzoic acid
-
10% residual acvtivity at 1 mM
3-(dihydroxyboryl)-5-(2-phenylacetamido)benzoic acid
-
3% residual acvtivity at 1 mM
3-(dihydroxyboryl)benzoic acid
-
20% residual acvtivity at 1 mM
3-(N-glycyl-L-cysteinyl)-N-ethylpropionamide
-
-
3-(trifluoroacetyl)benzoic acid
-
60% residual activity at 1 mM
3-formylbenzoic acid
-
77% residual activity at 1 mM
4-(dihydroxyboranyl)benzoic acid
-
85% residual activity at 1 mM
4-chloromercuribenzoate
-
complete inhibition at 1 mM
4-[(phenylacetyl)amino]-3-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)benzoic acid
-
81% residual activity at 1 mM
5-(dihydroxyboranyl)thiophene-2-carboxylic acid
-
57% residual activity at 1 mM
6-beta(alpha-N-acetyl-L-lysyl)-aminopenicillanic acid
-
-
6-beta(D-alpha-aminopimelyl)-aminopenicillanic acid
6-beta(D-alpha-aminosuberyl)-aminopenicillanic acid
6-beta(N-acetyl-L-alanyl-gamma-D-glutamyl-L-alanyl)-aminopenicillanic acid
-
-
7-(phenoxyacetamido)-3-desacetoxycephalosporamic acid
-
-
7-beta(alpha-N-acetyl-L-lysyl)-aminocephalosporanic acid
-
-
7-beta(D-alpha-aminopimelyl)-aminocephalosporanic acid
alpha-N-glycyl-epsilon-N-acetyl-L-lysine
-
inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
amoxicillin
-
-
ampicillin
arylakylidene imino-thiazolidin-4-ones 10
-
86% inhibition
arylakylidene imino-thiazolidin-4-ones 11
-
82% inhibition
arylakylidene imino-thiazolidin-4-ones 12
-
64% inhibition
arylakylidene imino-thiazolidin-4-ones 13
-
83% inhibition
arylakylidene imino-thiazolidin-4-ones 16
-
100% inhibition
arylakylidene imino-thiazolidin-4-ones 17
-
100% inhibition
arylakylidene imino-thiazolidin-4-ones 18
-
96% inhibition
arylakylidene imino-thiazolidin-4-ones 19
-
84% inhibition
arylakylidene imino-thiazolidin-4-ones 5
-
89% inhibition
arylakylidene imino-thiazolidin-4-ones 6
-
92% inhibition
arylakylidene imino-thiazolidin-4-ones 8
-
99% inhibition
arylakylidene imino-thiazolidin-4-ones 9
-
85% inhibition
arylalkylidene rhodanine derivative 1
-
88% inhibition
arylalkylidene rhodanine derivative 2
arylalkylidene rhodanine derivative 3
-
83% inhibition
arylalkylidene rhodanine derivative 4
-
95% inhibition
benzylpenicillin
benzylpenicllin
-
dipeptide-releasing activity is penicillin sensitive
-
beta-lactam
beta-Lactams
biphenyl-4,4'-diyldiboronic acid
-
97% residual activity at 0.1 mM
Boc-gamma-D-Glu-L-Lys-(Cbz)-D-boroAla-(-)-pinanediol
-
effective inhibitor of the D-alanine CPase activity of PBP5
carbenicillin
-
-
cefaclor
-
-
cefadroxil
-
-
cefalexin
-
-
cefalothin
-
-
cefmetazole
-
-
cephalexin
-
-
Cephalosporidin
-
-
cephalosporin
cephalosporin C
-
classical non-specific beta-lactam
Cephalothin
Cetyltrimethylammonium bromide
-
-
Cloxacillin
-
-
D-Ala(P,O)D-Ala
-
acts as slow binding inhibitor
D-Ala(P,O)D-Phe
-
acts as slow binding inhibitor
D-alpha-phenylpropionate
-
-
D-Cysteine
-
-
D-hexahydromandelate
-
-
D-mandelate
-
-
D-phenyllactate
-
-
Delta2-(and Delta3)-Deacetoxy-7-phenylacetamidocephalosporanates
-
-
-
depsipeptides
-
-
-
diacetyl-L-Lys-D-Ala
-
-
Dicloxacillin
-
-
diisopropyl fluorophosphate
-
1 mM, 28% inhibition
dithiothreitol
-
-
EDTA
Nonomuraea sp.
-
4 mM, complete loss of activity. Ativity may be restored in presence of 8 mM Zn2+
epsilon-N-glycyl-alpha-N-acetyl-L-alanyl-D-isoglutaminyl-L-lysine
-
inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
formaldehyde
-
1 mM, 53% inhibition
glycolyl-L-Phe
-
-
iodoacetamide
-
1 mM, 20% inhibition
L-cysteine
-
-
Mercaptosuccinic acid
-
-
Methicillin
Moenomycin
N,N-diacetyl-L-Lys-D-Ala-D-Ala
Streptomyces pneumoniae
-
inhibition of PBP3 by its own substrate above a ligand concentration of 15 mM
N-(glycyl)-D,L-alpha-aminocaprylic acid
-
inhibits hydrolysis of 3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
N-benzoyl-beta-sultam
-
time-dependent, irreversible active site directed inhibitor, the rate of inactivation is first order with respect to beta-sultam concentration and second order rate constants show dependence on pH
N-Chlorosuccinimide
-
1 mM, 50% inhibition
N-p-chloro-beta-sultam
-
-
N-p-methoxy-beta-sultam
-
least effective inhibitor
N-p-nitro-beta-sultam
-
-
Nalpha-tert-butoxycarbonyl-Nepsilon-benzyloxycarbonyl-L-Lys-D-Ala-D-Ala
-
substrate inhibition above 30 mM
NEM
-
1 mM, complete inhibition
nocardicin A
Oxyimino-DELTA3-cephalosporins
p-hydroxymercuribenzoate
-
1 mM, complete inhibition
Penicillanate
-
-
penicillin
penicillin G
Penicillin V
-
-
phenoxymethylpenicillin
-
-
phenyl glycyl-L-alpha-aminopimelyl-epsilon-(D-2-aminoethyl)phosphonate
-
-
phenylboronic acid
piperacillin
sulfazecine
thiophen-2-ylboronic acid
-
88% residual activity at 1 mM
thiosalicylic acid
-
-
Vancomycin
-
vancomycin derivatives inhibit the glycosyl transferase activity
[3-([[5-(dimethylamino)naphthalen-1-yl]sulfonyl]amino)phenyl]boronic acid
-
60% residual activity at 0.5 mM
[3-[(2,6-dimethoxybenzoyl)amino]-5-(methoxycarbonyl)thiophen-2-yl]boronic acid
-
98% residual activity at 1 mM
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
-
activation
aminomalon-(N-ethyl)amide
-
dual function acceptor, which is particularly effective
aminomalon-(N-phenethyl)amide
-
dual function acceptor, which is particularly effective
D-phenylalanine
-
most effective D-amino acid and dipeptide acceptor, respectively
Gly-L-Phe
-
most effective D-amino acid and dipeptide acceptor, respectively
N-ethyl-D-asparagine
-
less effective acceptor
p-chloromercuribenzoate
-
stimulation of hydrolase reaction, no influence on transpeptidase
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
(2R)-2-[[N-(phenylacetyl)glycyl]sulfanyl]propanoic acid
-
-
0.86
2-(N-glycyl-L-cysteinyl)acetyl-D-alanyl-D-alanine
-
-
1.28
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine
-
-
3
3-(N-glycyl-cysteaminyl)propanoyl-D-alanyl-D-alanine
-
-
0.004
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
-
-
0.48
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanyl-D-alanine
-
-
0.26
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine
-
-
0.36 - 4.3
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
0.2 - 0.45
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
0.45
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
-
-
0.35 - 1.8
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
1.37
3-[[N-(phenylacetyl)-D-alanyl]oxy]benzoic acid
-
-
0.76
3-[[N-(phenylacetyl)glycyl]oxy]benzoic acid
-
-
7.5
acetyl-L-Ala-D-Glu-L-Lys(Ac)-D-Ala-D-Ala
-
-
8
acetyl-L-Lys-D-Ala-D-Ala
Nonomuraea sp.
-
pH 7.4, 25C
-
1.3 - 1.5
benzoyl-D-Ala-thioglycolate
0.8 - 3
benzoyl-Gly-thioglycolate
0.22 - 1
benzoyl-Gly-thiolactate
6.4
Boc-gamma-D-Glu-L-Lys-(Cbz)-D-Ala-D-Ala
-
-
20
D-Ala-D-Ala
Nonomuraea sp.
-
pH 7.4, 25C
0.86
Gly-gamma-L-Glu-D-Ala-D-Ala
-
-
0.0079
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala-D-Ala
0.26
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]amino]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
-
-
0.0041
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
-
-
0.12
hippuryl-mercaptoacetic acid
-
crystal and solution
1.38
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala
-
recombinant PBP 5, in 50 mM Tris-HCl, pH 8.5, at 37C
9.8
N,N'-diacetyl-L-lysyl-D-alanyl-D-alanine
-
-
3.5
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine
-
pH 7.5, 25C
0.675 - 0.913
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
0.0178 - 0.401
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
0.4
N-alpha-acetyl-L-Lys-D-Ala-thiolactate
pH 7.2, 37C, wild-type enzyme
0.0079
N-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]-D-alanyl-D-alanine
-
-
3.7
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine
-
pH 7.5, 25C
12
Nalpha, Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
-
lysozyme releasable enzyme
0.1 - 14
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
2 - 3
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate
1.45
Nalpha-(beta-1,4 acetylglucosaminyl-N-acetylmuramyl-L-alanyl-D-isoglutaminyl)-Nepsilon-(D-isoasparaginyl)L-Lys-D-Ala-D-Ala
-
-
-
9.4 - 30
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala
0.6
Nalpha-acetyl-L-Lys-D-Ala-thiolactate
pH 7.2, 37C, mutant enzyme C98A
2.4 - 3
phenylacetyl-D-Ala-thioglycolate
1.2
phenylacetyl-D-Ala-thiolactate
pH 7.2, 37C, mutant enzyme C98A; pH 7.2, 37C, wild-type enzyme
1.4
UDP-N-acetylmuramyl-L-Ala-D-Glu-mesodiaminopimelyl-D-Ala-D-Ala
-
-
5 - 20
X-D-Ala-D-Ala
-
value depending on X
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.7
(2R)-2-[[N-(phenylacetyl)glycyl]sulfanyl]propanoic acid
Streptomyces sp. R61
-
-
0.17
2-(N-glycyl-L-cysteinyl)acetyl-D-alanyl-D-alanine
Streptomyces sp. R61
-
-
0.25
3-(N-acetyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine
Streptomyces sp. R61
-
-
2.4
3-(N-glycyl-cysteaminyl)propanoyl-D-alanyl-D-alanine
Streptomyces sp. R61
-
-
42
3-(N-glycyl-L-cysteinyl)-propanoyl-D-alanyl-D-thiolactate
Streptomyces sp. R61
-
-
0.27
3-(N-glycyl-L-cysteinyl)butanoyl-D-alanyl-D-alanine
Streptomyces sp. R61
-
-
48
3-(N-glycyl-L-cysteinyl)propanoyl-D-alanyl-D-alanine
Streptomyces sp. R61
-
-
6.9 - 10.4
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
4 - 114
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
44 - 114
3-carboxyphenyl N-(phenylacetyl)-alpha-methoxyglycinate
0.65 - 22
3-carboxyphenyl N-(phenylacetyl)-alpha-serinate
31
3-[[N-(phenylacetyl)-D-alanyl]oxy]benzoic acid
Streptomyces sp. R61
-
-
1.51
3-[[N-(phenylacetyl)glycyl]oxy]benzoic acid
Streptomyces sp. R61
-
-
12.5
acetyl-L-Lys-D-Ala-D-Ala
Nonomuraea sp.
-
pH 7.4, 25C
-
0.11 - 0.3
benzoyl-D-Ala-thioglycolate
0.024 - 0.08
benzoyl-Gly-thioglycolate
0.006 - 0.08
benzoyl-Gly-thiolactate
8.3
D-Ala-D-Ala
Nonomuraea sp.
-
pH 7.4, 25C
17.5 - 350
diacetyl-L-Lys-D-Ala-D-Ala
12.8
Gly-gamma-L-Glu-D-Ala-D-Ala
Streptomyces sp. R61
-
-
69
Gly-L-alpha-amino-epsilon-pimelyl-D-Ala-D-Ala
48
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]amino]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
Streptomyces sp. R61
-
-
81
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
Streptomyces sp. R61
-
-
1.41
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala
Escherichia coli
-
recombinant PBP 5, in 50 mM Tris-HCl, pH 8.5, at 37C
34.5
N,N'-diacetyl-L-lysyl-D-alanyl-D-alanine
Streptomyces sp. R61
-
-
0.9
N-(N-acetyl-1-O-methyl-beta-muramoyl)-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine
Escherichia coli
-
pH 7.5, 25C
2.51 - 3.18
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
2.42 - 3.47
N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine
0.1 - 0.8
N-alpha-acetyl-L-Lys-D-Ala-thiolactate
69
N-[(6S)-6-carboxy-6-(glycylamino)hexanoyl]-D-alanyl-D-alanine
Streptomyces sp. R61
-
-
1.1
N-[N-acetyl-4-O-[2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl]-1-O-methyl-beta-muramoyl]-L-alanyl-D-gamma-glutamyl-L-lysyl-D-alanyl-D-alanine
Escherichia coli
-
pH 7.5, 25C
0.2 - 2.7
Nalpha,Nepsilon-Diacetyl-L-Lys-D-Ala-D-Ala
0.43 - 1.1
Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-thiolactate
0.13 - 0.45
Nalpha-Acetyl-L-Lys-D-Ala-D-Ala
0.015 - 6.08
phenylacetyl-D-Ala-thioglycolate
0.6 - 2.2
phenylacetyl-D-Ala-thiolactate
additional information
additional information
Escherichia coli
-
the ratio of turnover number to Km-value for the substrate Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala is 32/M*s for the wild-type enzyme, 3.9/M*s for the S86A mutant, 2.0/M*s for the S87A mutant and 2.6/M*s for the S86A/S87A mutant enzyme
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
19 - 24
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-hydroxyglycinate
98 - 570
3-carboxyphenyl (2R)-N-(phenylacetyl)-2-methoxyglycinate
1.56
acetyl-L-Lys-D-Ala-D-Ala
Nonomuraea sp.
-
pH 7.4, 25C
202289
0.415
D-Ala-D-Ala
Nonomuraea sp.
-
pH 7.4, 25C
1348
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00037
(2R)-2-amino-7-oxo-7-[(4,4,4-trifluoro-3-oxobutan-2-yl)amino]heptanoic acid
-
pH not specified in the publication, temperature not specified in the publication
1.02
3-(N-glycyl-L-cysteinyl)-N-ethylpropionamide
-
-
0.013
Boc-gamma-D-Glu-L-Lys-(Cbz)-D-boroAla-(-)-pinanediol
-
-
15.5
D-alpha-phenylpropionate
-
glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
7
D-hexahydromandelate
-
substrate glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
11.6
D-mandelate
-
substrate (2R)-2-[[N-(phenylacetyl)glycyl]sulfanyl]propanoic acid; substrate 3-[[N-(phenylacetyl)-D-alanyl]oxy]benzoic acid; substrate 3-[[N-(phenylacetyl)glycyl]oxy]benzoic acid; substrate glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
17.8
D-phenyllactate
-
substrate glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
19.3
glycolyl-L-Phe
-
substrate glycyl-S-(3-[[(1R)-2-[[(1R)-1-carboxyethyl]sulfanyl]-1-methyl-2-oxoethyl]amino]-3-oxopropyl)-L-cysteine
additional information
additional information
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
28
(3R,5R)-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
1
(3R,5R,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
25
(3R,5R,6S)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
6.6
(3R,5S)-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
4.5
(3R,5S,6R)-3-benzyloxycarbonyl-6-methyl-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
3.4
(3R,5S,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
1.3
(3S,5R)-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
16
(3S,5R,6S)-3-benzyloxycarbonyl-6-methyl-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
5.4
(3S,5R,6S)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
3
(3S,5S,6R)-6-methyl-3-(furyl-2')-4-dethia-4-oxa-penam
Saccharopolyspora erythraea
-
-
1
3-(acetylamino)-5-(dihydroxyboranyl)benzoic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
0.034
3-(benzoylamino)-5-(dihydroxyboranyl)benzoic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
0.028
3-(dihydroxyboranyl)-5-[(phenoxyacetyl)amino]benzoic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
0.078
3-(dihydroxyboranyl)-5-[(thiophen-2-ylacetyl)amino]benzoic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
0.032
3-(dihydroxyboranyl)-5-[(thiophen-2-ylcarbonyl)amino]benzoic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
0.023
3-(dihydroxyboryl)-5-(2-methoxybenzamido)benzoic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
0.088
3-(dihydroxyboryl)-5-(2-phenylacetamido)benzoic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
0.4
3-(dihydroxyboryl)benzoic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
0.06
ampicillin
Corynebacterium jeikeium
-
-
0.5
phenylboronic acid
Actinomadura sp. R39
-
in 10 mM sodium phosphate buffer (pH 7.2) with 100 mM NaCl, 100 mM D-alanine, and 0.01 mg/ml bovine serum albumin for 60 min at 25C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 8
Nonomuraea sp.
-
substrate Nalpha,Nepsilon-diacetyl-L-Lys-D-Ala-D-Ala , pH 7.4, 25C
18
Nonomuraea sp.
-
substrate D-Ala-D-Ala, pH 7.4, 25C
40
Nonomuraea sp.
-
substrate acetyl-L-Lys-D-Ala-D-Ala, pH 7.4, 25C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
-
vegetative and stage-V forespore membrane DD-carboxypeptidase
6 - 8
-
hydrolysis, value increased by high ionic strength
6
-
sodium cacodylate buffer
6 - 8
-
hydrolysis, value increased by high ionic strength
7.6
activity assay
9.5
-
transpeptidation, Streptomyces sp. R39
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.6 - 8.9
-
effectiveness of D-alanine as an acceptor increases with pH
8 - 11
-
pH 8: about 50% of maximal activity, pH 11: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
kinetic measurements
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
PBP3 is evenly distributed on both hemispheres but absent at the equator, the site of future division
Manually annotated by BRENDA team
mutant enzyme S96A is produced in cytoplasm of Escherichia coli
Manually annotated by BRENDA team
-
anchored into the outer leaflet of the cytoplasmic membrane
-
Manually annotated by BRENDA team
Nonomuraea sp.
-
-
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Actinomadura sp. (strain R39)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bacillus subtilis (strain 168)
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Bdellovibrio bacteriovorus (strain ATCC 15356 / DSM 50701 / NCIB 9529 / HD100)
Enterococcus faecalis (strain ATCC 700802 / V583)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)
Pseudomonas aeruginosa (strain UCBPP-PA14)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain COL)
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Staphylococcus aureus (strain Mu50 / ATCC 700699)
Streptococcus pneumoniae (strain ATCC BAA-255 / R6)
Streptomyces sp. (strain K15)
Streptomyces sp. (strain K15)
Streptomyces sp. (strain K15)
Streptomyces sp. (strain K15)
Streptomyces sp. (strain K15)
Streptomyces sp. (strain K15)
Streptomyces sp. (strain K15)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)
Streptomyces sp. (strain R61)