Information on EC 3.4.15.5 - Peptidyl-dipeptidase Dcp

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.15.5
-
RECOMMENDED NAME
GeneOntology No.
Peptidyl-dipeptidase Dcp
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of unblocked, C-terminal dipeptides from oligopeptides, with broad specificity. Does not hydrolyse bonds in which P1' is Pro, or both P1 and P1' are Gly
show the reaction diagram
broad specificity, does not hydrolyze bonds in which P1' is Pro, or both P1 and P1' are Gly
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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-
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CAS REGISTRY NUMBER
COMMENTARY hide
9015-82-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-Nitrobenzyloxycarbonyl-Gly-(S-4-nitrobenzo-2-oxa-1,3-diazole)-Cys-Gly + H2O
?
show the reaction diagram
-
-
-
-
-
4-Nitrobenzyloxycarbonyl-Gly-Phe-Gly + H2O
?
show the reaction diagram
-
-
-
-
-
Acetyl-Ala-Ala-Ala + H2O
Acetyl-Ala + Ala-Ala
show the reaction diagram
Acetyl-Ala3 + H2O
?
show the reaction diagram
Ala-Ala-Ala-Ala + H2O
Ala-Ala + Ala-Ala
show the reaction diagram
-
-
-
-
Ala-Ala-Ala-Lys-Phe + H2O
Ala-Ala-Ala + Lys-Phe
show the reaction diagram
-
-
-
-
angiotensin I + H2O
DRVYIHPF + His-Leu
show the reaction diagram
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i.e. DRVYIHPFHL, hydrolysis at the F-H bond generating angiotensin II
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-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
?
show the reaction diagram
-
-
-
-
-
Benzyloxycarbonyl-Ala-Ala-Ala + H2O
Benzyloxycarbonyl-Ala + Ala-Ala
show the reaction diagram
-
-
-
-
bradykinin + H2O
RPPGF + Ser-Pro + Phe-Arg
show the reaction diagram
-
i.e. RPPGFSPFR, hydrolysis at at sequential cleavage sites releasing the C-terminal dipeptides F-R and S-P
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-
?
Dinitrophenyl-poly-(Pro-Gly-Pro) + H2O
Gly-Pro + ?
show the reaction diagram
-
-
-
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Gly-Gly-Phe-Ala + H2O
Gly-Gly + Phe-Ala
show the reaction diagram
-
-
-
-
Hippuric acid-His-Leu + H2O
Hippuric acid + His-Leu
show the reaction diagram
-
-
-
-
Lys-Ala-Ala-Lys-Ala-Ala + H2O
Lys-Ala + Ala-Lys-Ala-Ala
show the reaction diagram
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-
-
-
Lys-Lys-Lys-Lys + H2O
Lys-Lys + Lys-Lys
show the reaction diagram
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-
-
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o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-OH + H2O
o-aminobenzoyl-FR + AK(2,4-dinitrophenyl)-OH
show the reaction diagram
-
an ACE substrate, Dcp hydrolyzes at the R-A bond
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-
?
o-aminobenzoyl-FRK(2,4-dinitrophenyl)P-OH + H2O
o-aminobenzoyl-FR + K(2,4-dinitrophenyl)P-OH
show the reaction diagram
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an ACE substrate, Dcp hydrolyzes at the R-K bond
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-
?
o-aminobenzoyl-LFK(2,4-dinitrophenyl)-OH + H2O
o-aminobenzoyl-L + FK(2,4-dinitrophenyl)-OH
show the reaction diagram
-
an ACE substrate, Dcp hydrolyzes at the L-F bond
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-
?
tert-Butyloxycarbonyl-Ala-Glu-Ala-Ala + H2O
tert-Butyloxycarbonyl-Ala-Glu + Ala-Ala
show the reaction diagram
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-
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additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Acetyl-Ala3 + H2O
?
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
activates
Mn2+
-
activates
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(5S)-5-[(N-benzoyl)amino]-4-oxo-6-phenylhexanoyl-L-tryptophan
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-
1,10-phenanthroline
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captopril
chymostatin
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lisinopril
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lisinopril-Trp
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Pepstatin
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trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butane
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i.e. E-64
Trypsin inhibitor
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from lima bean and from bovine pancreas
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additional information
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inhibition mechanism, molecular modeling, overview
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.27
Ala-Ala-Phe-Ala
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-
0.61
Ala-Gly-Phe-Ala
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-
0.44
Ala4
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-
1.39
Gly-Ala-Phe-Ala
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-
6.06
Gly-Gly-Phe-Ala
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-
0.1
Lys4
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-
0.00066
o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-OH
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pH 8.0, 37°C, recombinant enzyme
0.00029
o-aminobenzoyl-FRK(2,4-dinitrophenyl)P-OH
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pH 8.0, 37°C, recombinant enzyme
0.00255
o-aminobenzoyl-LFK(2,4-dinitrophenyl)-OH
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pH 8.0, 37°C, recombinant enzyme
0.71
tert-butyloxycarbonyl-Ala3
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-
additional information
additional information
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kinetics, overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.1
o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-OH
Escherichia coli
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pH 8.0, 37°C, recombinant enzyme
0.81
o-aminobenzoyl-FRK(2,4-dinitrophenyl)P-OH
Escherichia coli
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pH 8.0, 37°C, recombinant enzyme
1.4
o-aminobenzoyl-LFK(2,4-dinitrophenyl)-OH
Escherichia coli
-
pH 8.0, 37°C, recombinant enzyme
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1667
o-aminobenzoyl-FRAK(2,4-dinitrophenyl)-OH
Escherichia coli
-
pH 8.0, 37°C, recombinant enzyme
40530
2793
o-aminobenzoyl-FRK(2,4-dinitrophenyl)P-OH
Escherichia coli
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pH 8.0, 37°C, recombinant enzyme
40528
538
o-aminobenzoyl-LFK(2,4-dinitrophenyl)-OH
Escherichia coli
-
pH 8.0, 37°C, recombinant enzyme
40529
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.022
(5S)-5-[(N-benzoyl)amino]-4-oxo-6-phenylhexanoyl-L-tryptophan
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-
0.000003
captopril
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0.0044
lisinopril
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-
0.0008
lisinopril-Trp
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-
additional information
additional information
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inhibition kinetics, overview
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pI: 5.29, Escherichia coli, 5.06, Salmonella typhimurium
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
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activity range, profile, overview
5 - 7.5
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pH 5.0: 50% of activity maximum, pH 7.5: activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 42
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37°C: 75% of activity maximum, 42°C: activity maximum
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Escherichia coli (strain K12)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
77270
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Salmonella typhimurium, calculation from nucleotide sequence
77500
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E. coli, calculation from nucleotide sequence
78000
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1 * 78000, SDS-PAGE
97000
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E. coli B, gradient PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
-
peptide mapping, overview
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
crystallization in presence of a octapeptide inhibitor, sitting-drop vapor-diffusion procedure, 2.0 A resolution, indication of a ligand-dependent hinge movement mechanism
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, retains 70% of the original activity after 5 weeks
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged Dcp from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
phylogenetic analysis, expression of His-tagged Dcp in Escherichia coli strain BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
H466A/H470A/Q467A
mutation results in loss of activity
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