Information on EC 3.4.14.13 - gamma-D-glutamyl-L-lysine dipeptidyl-peptidase

for references in articles please use BRENDA:EC3.4.14.13
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The expected taxonomic range for this enzyme is: Bacillus

EC NUMBER
COMMENTARY hide
3.4.14.13
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RECOMMENDED NAME
GeneOntology No.
gamma-D-glutamyl-L-lysine dipeptidyl-peptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
The enzyme releases L-Ala-gamma-D-Glu dipeptides from cell wall peptides via cleavage of an L-Ala-gamma-D-Glu-/-L-Lys bond
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-Ala-gamma-D-Glu-L-Lys + H2O
L-Ala-D-Glu + L-Lys
show the reaction diagram
L-Ala-gamma-D-Glu-L-Lys-D-Ala + H2O
L-Ala-D-Glu + L-Lys-D-Ala
show the reaction diagram
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O
L-Ala-D-Glu + L-Lys-D-Ala-D-Ala
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala + H2O
L-Ala-D-Glu + L-Lys-D-Ala-D-Ala
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.29
L-Ala-gamma-D-Glu-L-Lys
pH and temperature not specified in the publication
0.12
L-Ala-gamma-D-Glu-L-Lys-D-Ala
pH and temperature not specified in the publication
0.31
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala
pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
L-Ala-gamma-D-Glu-L-Lys
pH and temperature not specified in the publication
2.6
L-Ala-gamma-D-Glu-L-Lys-D-Ala
pH and temperature not specified in the publication
5.7
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala
pH and temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.1
L-Ala-gamma-D-Glu-L-Lys
pH and temperature not specified in the publication
22
L-Ala-gamma-D-Glu-L-Lys-D-Ala
pH and temperature not specified in the publication
18
L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala
pH and temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
structure of the gamma-D-glutamyl-L-diamino acid endopeptidase YkfC from Bacillus cereus in complex with L-Ala-gamma-D-Glu, 1.8 A resolution
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 40% glycerol, significant loss of activity
4°C, stable in absence of glycerol for 48h
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
His-tagged enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
selenomethionine derivative of BcYkfC is expressed in Escherichia coli with an N-terminal TEV-cleavable His tag