Information on EC 3.4.14.1 - dipeptidyl-peptidase I

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The expected taxonomic range for this enzyme is: Eukaryota

EC NUMBER
COMMENTARY hide
3.4.14.1
-
RECOMMENDED NAME
GeneOntology No.
dipeptidyl-peptidase I
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Release of an N-terminal dipeptide, Xaa-Yaa-/-Zaa-, except when Xaa is Arg or Lys, or Yaa or Zaa is Pro
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
polymerization of dipeptide amides
-
-
-
-
transamidation
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-
CAS REGISTRY NUMBER
COMMENTARY hide
9032-68-2
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
under native conditions the enzyme may be aggregated with beta-hexosaminidase, beta-galactosidase and alpha-fucosidase
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
giant tiger prawn
UniProt
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
mutations in the cathepsin C gene result in an autosomal recessive disorder, Papillon-Lefevre syndrome
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(H2N-Abu-Homo-Phe)2-rhodamine + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
(H2N-fulleroproline-Homo-Phe)2-rhodamine + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
(H2N-Leu-Homo-Phe)2-rhodamine + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
(H2N-Leu-Leu)2-rhodamine + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
(H2N-Nva-(4-phenyl)Phe)2-rhodamine + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
(H2N-Nva-Homo-Phe)2-rhodamine + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
(H2N-Pro-Homo-Phe)2-rhodamine + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
(Pro-Arg)2-Rho + 2 H2O
rhodamine + 2 Pro-Arg
show the reaction diagram
-
-
-
-
?
Ala-Ala-2-naphthylamide + H2O
Ala-Ala + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Ala-Arg-2-naphthylamide + H2O
Ala-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Ala-Arg-NH2 + H2O
Ala-Arg + NH3
show the reaction diagram
-
-
-
-
?
Ala-Leu-NH2 + H2O
Ala-Leu + NH3
show the reaction diagram
-
-
-
-
?
Ala-Tyr-NH2 + H2O
Ala-Tyr + NH3
show the reaction diagram
-
-
-
-
?
alpha-Asp-Arg-2-naphthylamide + H2O
alpha-Asp-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
alpha-D-Asp1-angiotensin II + H2O
?
show the reaction diagram
-
low reaction rate
-
-
?
Asn1-angiotensin II + H2O
?
show the reaction diagram
-
-
-
-
?
benzyloxycarbonyl-Phe-Arg-4-methylcoumarin 7-amide + H2O
?
show the reaction diagram
-
-
-
-
?
beta-Asp1-angiotensin II + H2O
?
show the reaction diagram
-
low reaction rate
-
-
?
beta-corticotropin + H2O
Ser-Tyr + Ser-Met + Glu-His + Phe-Arg + Trp-Gly
show the reaction diagram
Glu-His-2-naphthylamide + H2O
Glu-His + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
glucagon + H2O
His-Ser + Thr-Phe + Thr-Ser + Asp-Tyr + Ser-Lys + Tyr-Leu + Asp-Ser + ?
show the reaction diagram
-
-
further degradation of the hormone is prevented by the appearance of the NH2-terminal Arg
?
Gly-(beta-phenyl)-L-lactic acid methyl ester + H2O
Gly-(beta-phenyl)-L-lactic acid + methanol
show the reaction diagram
-
-
-
?
Gly-Ala-NH2 + H2O
Gly-Ala + NH3
show the reaction diagram
-
-
-
-
?
Gly-Arg-2-naphthylamide + H2O
(Gly-Arg-)5-2-naphthylamide + 2-naphthylamine
show the reaction diagram
-
polymerization
polymers up to (Gly-Arg-)5-2-naphthylamide
?
Gly-Arg-2-naphthylamide + H2O
Gly-Arg + 2-naphthylamine
show the reaction diagram
Gly-Arg-4-methoxy-2-naphthylamide + H2O
Gly-Arg + 4-methoxy-2-naphthylamine
show the reaction diagram
-
-
-
-
?
Gly-Arg-4-methylcoumarin 7-amide + H2O
Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Gly-Arg-7-amido-4-methylcoumarin + H2O
Gly-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Gly-Arg-NH2 + H2O
?
show the reaction diagram
-
polymerization
-
-
?
Gly-Arg-p-nitroanilide + H2O
Gly-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Gly-Gly-ethyl ester + H2O
Gly-Gly + ethanol
show the reaction diagram
-
-
-
?
Gly-Gly-Gly + H2O
Gly-Gly + Gly
show the reaction diagram
-
-
-
?
Gly-Gly-Phe-NH2 + H2O
Gly-Gly + Phe-NH2
show the reaction diagram
-
-
-
-
?
Gly-L-Arg-7-amido-4-methylcoumarin + H2O
Gly-L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Gly-L-Phe-NH2 + H2O
Gly-L-Phe + NH3
show the reaction diagram
Gly-L-Phe-NH2 + H2O
Gly-Phe-Gly-Phe-Gly-Phe-Gly-Phe-NH2
show the reaction diagram
Gly-L-Trp-NH2 + H2O
Gly-L-Trp + NH3
show the reaction diagram
-
-
-
?
Gly-L-Tyr-NH2 + H2O
Gly-L-Tyr + NH3
show the reaction diagram
Gly-Leu-ethyl ester + H2O
Gly-Leu + ethanol
show the reaction diagram
-
-
-
-
?
Gly-Leu-NH2 + H2O
Gly-Leu + NH3
show the reaction diagram
-
-
-
-
?
Gly-Phe-2-naphthylamide + H2O
2-naphthylamine + Gly-Phe
show the reaction diagram
-
assay at 37C, pH 5.5, reaction stopped by addition of glycine-NaOH
-
-
?
Gly-Phe-2-naphthylamide + H2O
Gly-Phe + 2-naphthylamine
show the reaction diagram
Gly-Phe-4-methoxy-2-naphthylamide + H2O
Gly-Phe + 4-methoxy-2-naphthylamine
show the reaction diagram
-
-
-
-
?
Gly-Phe-7-amido-4-methylcoumarin + H2O
Gly-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
Gly-Phe-ethyl ester + H2O
Gly-Phe + ethanol
show the reaction diagram
-
-
-
?
Gly-Phe-methyl ester + H2O
Gly-Phe + methanol
show the reaction diagram
-
-
-
-
?
Gly-Phe-p-nitroanilide + H2O
Gly-Phe + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Gly-Trp-2-naphthylamide + H2O
Gly-Trp + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Gly-Trp-methyl ester + H2O
Gly-Trp + methanol
show the reaction diagram
-
-
-
-
?
Gly-Trp-NH2 + H2O
?
show the reaction diagram
-
polymerization
-
-
?
Gly-Tyr(3'NO2)-Gly-Pro-Pro-Lys(epsilon-(2-aminobenzoyl))-Gly + H2O
Gly-Tyr(3'NO2) + Gly-Pro-Pro-Lys(epsilon-(2-aminobenzoyl))-Gly
show the reaction diagram
-
-
-
-
?
Gly-Tyr-ethyl ester + H2O
Gly-Tyr + ethanol
show the reaction diagram
-
-
-
-
?
Gly-Tyr-Gly + H2O
Gly-Tyr + Gly
show the reaction diagram
-
-
-
-
?
H2N-Abu-(4-phenyl)Phe-rhodamine-morpholine-4-carboxamide + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
H2N-Abu-Homo-Phe-rhodamine-morpholine-4-carboxamide + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
H2N-fulleroproline-(3-methyl)Phe-rhodamine-morpholine-4-carboxamide + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
H2N-fulleroproline-(4-phenyl)Phe-rhodamine-morpholine-4-carboxamide + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
H2N-Leu-(3-methyl)Phe-rhodamine-morpholine-4-carboxamide + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
H2N-Nva-(3-methyl)Phe-rhodamine-morpholine-4-carboxamide + H2O
?
show the reaction diagram
-
assay at pH 5.5, 37C
-
-
?
hexaalanine + H2O
Ala-Ala
show the reaction diagram
-
-
-
?
His-Leu-NH2 + H2O
His-Leu + NH3
show the reaction diagram
-
-
-
-
?
His-Ser-2-naphthylamide + H2O
His-Ser + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
His-Tyr-NH2 + H2O
His-Tyr + NH3
show the reaction diagram
-
-
-
-
?
Ile-Leu-NH2 + H2O
Ile-Leu + NH3
show the reaction diagram
-
-
-
-
?
Ile5-angiotensin II + H2O
?
show the reaction diagram
-
-
-
-
?
Insulin B-chain + H2O
?
show the reaction diagram
L-His-L-Phe-NH2 + H2O
L-His-L-Phe + NH3
show the reaction diagram
-
-
-
?
L-His-L-Tyr-NH2 + H2O
L-His-L-Tyr + NH3
show the reaction diagram
-
-
-
?
L-Ser-L-Tyr-7-amido-4-methylcoumarin + H2O
L-Ser-L-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Leu-enkephalin + H2O
Tyr-Gly + Gly-Phe-Leu
show the reaction diagram
-
i.e. Tyr-Gly-Gly-Phe-Leu
no further degradation of the tripeptide
?
Leu-Tyr-NH2 + H2O
Leu-Tyr + NH3
show the reaction diagram
-
-
-
-
?
Lys-Gly-NH2 + H2O
Lys-Gly + NH3
show the reaction diagram
-
-
-
-
?
Met-enkephalin + H2O
Tyr-Gly + Gly-Phe-Met
show the reaction diagram
-
-
further degradation of Gly-Phe-Met to Gly-Phe + Met
?
Met-Met-Met + H2O
Met-Met + Met
show the reaction diagram
-
-
-
?
pentaalanine + H2O
Ala-Ala + Ala-Ala-Ala
show the reaction diagram
-
-
tri-alanine is resistant to further breakdown
?
Phe-Arg-2-naphthylamide + H2O
Phe-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Phe-Phe-Phe + H2O
Phe-Phe + Phe
show the reaction diagram
-
-
-
?
Pro-Arg-2-naphthylamide + H2O
Pro-Arg + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Pro-Arg-7-amido-4-methylcoumarin + H2O
Pro-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Pro-Leu-NH2 + H2O
Pro-Leu + NH3
show the reaction diagram
-
-
-
-
?
Pro-Phe-NH2 + H2O
Pro-Phe + NH3
show the reaction diagram
sarcosyl-Phe-ethyl ester + H2O
sarcosyl-Phe + ethanol
show the reaction diagram
-
-
-
-
?
secretin + H2O
His-Ser + Asp-Gly + Thr-Phe + Thr-Ser + Glu-Leu + Ser-Arg + Leu-Arg + Asp-Ser + Ala-Arg + Leu-Gln + ?
show the reaction diagram
-
-
further degradation of the hormone is prevented by the appearance of the NH2-terminal Arg
?
Ser-His-Ala + H2O
Ser-His + Ala
show the reaction diagram
-
-
-
?
Ser-Leu-NH2 + H2O
Ser-Leu + NH3
show the reaction diagram
-
-
-
-
?
Ser-Met-2-naphthylamide + H2O
Ser-Met + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Ser-Met-Glu + H2O
Ser-Met + Glu
show the reaction diagram
-
-
-
?
Ser-Tyr-2-naphthylamide + H2O
Ser-Tyr + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Ser-Tyr-NH2 + H2O
Ser + Tyr + NH3
show the reaction diagram
-
-
-
-
?
tetracosactrin + H2O
?
show the reaction diagram
tetraglycine + H2O
Gly-Gly
show the reaction diagram
-
-
-
?
tetraphenylalanine + H2O
Phe-Phe
show the reaction diagram
-
-
-
?
Thr-Leu-NH2 + H2O
Thr-Leu + NH3
show the reaction diagram
-
-
-
-
?
thyroglobulin + H2O
?
show the reaction diagram
-
-
enzyme removes up to 12 amino acids from the N-terminus of porcine thyroglobulin, including a dipeptide with thyroxin on position 5. The newly formed N-terminus, Arg-Pro-, is not hydrolysed further
-
?
Val-Leu-NH2 + H2O
Val-Leu + NH3
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F-
-
absolute requirement for halide ions, the efficacy in decreasing order: Cl-, Br-, I-, F-
I-
-
absolute requirement for halide ions, the efficacy in decreasing order: Cl-, Br-, I-, F-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2S)-2-amino-N-[(1S)-2-(biphenyl-4-yl)-1-cyanoethyl]butanamide
-
-
(4R)-N-[(1S)-1-cyano-2-phenylethyl]-4-(methylsulfanyl)-4-phenyl-L-prolinamide
-
-
(4R)-N-[(1S)-1-cyano-2-phenylethyl]-4-fluoro-4-phenyl-L-prolinamide
-
-
(4S)-4-chloro-N-[(1S)-1-cyano-2-phenylethyl]-L-prolinamide
-
-
(4S)-N-(1-cyanocyclopropyl)-4-(methylsulfanyl)-L-prolinamide
-
-
(4S)-N-(1-cyanocyclopropyl)-4-fluoro-L-prolinamide
-
-
(4S)-N-[(1R,2S)-2-benzyl-1-cyanocyclopropyl]-4-(methylsulfanyl)-L-prolinamide
-
-
(4S)-N-[(1R,2S)-2-benzyl-1-cyanocyclopropyl]-4-fluoro-L-prolinamide
-
-
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-(ethylsulfanyl)-L-prolinamide
-
-
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-(methylsulfanyl)-L-prolinamide
-
-
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-fluoro-L-prolinamide
-
-
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-methoxy-L-prolinamide
-
-
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-methyl-L-prolinamide
-
-
(4S)-N-[(1S,2R)-2-benzyl-1-cyanocyclopropyl]-4-(methylsulfanyl)-L-prolinamide
-
-
(4S)-N-[(1S,2R)-2-benzyl-1-cyanocyclopropyl]-4-fluoro-L-prolinamide
-
-
(4S)-N-[(1S,2S)-1-cyano-2-phenylcyclopropyl]-4-fluoro-L-prolinamide
-
-
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(2-naphthyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
-
IC50: 19 nM
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(benzo[b]-thiophen-3-yl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
-
IC50: 22 nM
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(indol-3-yl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
-
IC50: 21 nM
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(m-fluorophenyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
-
IC50: 31 nM, competitive inhibition, selective for DPP I over other cysteine and serine proteases, noncytotoxic
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(m-methoxyphenyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
-
IC50: 39 nM
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(p-chlorophenyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
-
IC50: 45 nM
3-[4-(1-amino-1-cyclopentylethyl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
3-[4-(1-amino-2-phenylethyl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-(1-aminocyclohexyl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-(1-aminocyclohexyl)-1H-1,2,3-triazol-1-yl]-2-oxoheptyl 2,6-dimethylbenzoate
-
-
3-[4-(1-aminoethyl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-(2-aminopentan-2-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-(2-aminopropan-2-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-(2-methylpyrrolidin-2-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-(3-aminopentan-3-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-(4-aminoheptan-4-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-[(1R)-1-amino-1-cyclopentylethyl]-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-[(1S)-1-amino-1-cyclohexylethyl]-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-[(1S)-1-amino-1-cyclopentylethyl]-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
3-[4-[4-(methylamino)heptan-4-yl]-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
-
-
5-aminopentanoic acid ethyl ester
-
-
Ala-4(I)Phe-diazomethyl ketone
-
irreversible inhibitor
antipain
Arg-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
Benzoyl-Arg-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
beta-Ala-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
beta-alanine ethyl ester
-
-
Boc-Gly-DELTA(Z)Phe-AbuPO(OMe)2
-
-
Butylamine
-
reaction with Gly-Gly-ethyl ester, Gly-Gly-NH2 or Gly-Phe-NH2
Cystatin
-
-
-
ethylamine
-
-
fluoride
-
fluoride preparations inhibit activity of cathepsin C in saliva
FY01
-
FY01 is a selective reagent for DPPI and can efficiently label its target in an activity-dependent manner in both crude tissue extracts and intact cells
gamma-aminobutyryl ethyl ester
-
-
-
Glu-alpha-methyl ester
-
inhibition of the reaction with Gly-Gly-ethyl ester, weak inhibition of reaction with Gly-L-Phe-NH2
-
glucagon
-
competitive inhibition of His-Ser-2-naphthylamide hydrolysis
Gly-beta-Ala-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
Gly-DELTA(Z)Phe-AlaPO(OEt)2-trifluoroacetic acid
-
-
Gly-DELTAZPhe-Gly-DELTAEPhe-Gly
-
-
Gly-DELTAZPhe-Gly-DELTAEPhe-Gly-OMe
-
-
Gly-DELTAZPhe-Gly-DELTAEPhe-Phe
-
-
Gly-DELTAZPhe-Gly-DELTAEPhe-Phe-OMe
-
-
Gly-Gly-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
Gly-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
Gly-Phe-NH2
-
competitive inhibition of His-Ser-2-naphthylamide hydrolysis
glycinamide
-
reaction with Gly-Gly-ethyl ester, Gly-Gly-NH2 or Gly-Phe-NH2
glycine ethyl ester
-
-
Guanidinium chloride
-
reversible, significantly decreases the Km-value of substrate hydrolysis, without changing the maximal velocity
Hexylamine
-
-
iodoacetamide
iodoacetate
-
-
L-trans-epoxy-succinyl-leucylamido(4-guanidino)-butane
-
-
Leu-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
Leupeptin
Lys-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester, weak inhibition of reaction with Gly-L-Phe-NH2
methylamine
-
-
N-(1-cyano-2,2-dimethylcyclopropyl)-3-thiophen-2-yl-L-alaninamide
-
-
N-(1-cyano-2-phenylcyclopropyl)-3-thiophen-2-yl-L-alaninamide
-
-
N-(1-cyanocyclobutyl)-3-thiophen-2-yl-L-alaninamide
-
-
N-(1-cyanocyclopropyl)-3-thiophen-2-yl-L-alaninamide
-
-
N-(2-cyanopropan-2-yl)-3-thiophen-2-yl-L-alaninamide
-
-
N-(cyanomethyl)-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-norvalinamide
-
-
N-[(1R,2R)-1-cyano-2-phenylcyclopropyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2,2-dimethylpropyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2,2-diphenylethyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-L-methioninamide
-
-
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-L-valinamide
-
-
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-S-ethyl-L-cysteinamide
-
-
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-S-ethyl-L-homocysteinamide
-
-
N-[(1S)-1-cyano-2-(4-fluorophenyl)ethyl]-L-prolinamide
-
-
N-[(1S)-1-cyano-2-(5-phenylthiophen-2-yl)ethyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2-(naphthalen-2-yl)ethyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2-cyclohexylethyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2-methylpropyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2-phenylethyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-1-cyano-2-phenylethyl]-L-prolinamide
-
-
N-[(1S)-1-cyano-2-[4-(methylsulfanyl)phenyl]ethyl]-L-prolinamide
-
-
N-[(1S)-1-cyano-3-phenylpropyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S)-2-(1,3-benzothiazol-2-yl)-1-cyanoethyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[(1S,2S)-1-cyano-2-phenylcyclopropyl]-3-thiophen-2-yl-L-alaninamide
-
-
N-[2-(1H-indol-3-yl)ethyl]-L-methioninamide
-
-
N2-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-leucinamide
-
-
p-Hydroxymercuriphenyl sulfonate
-
-
PCMB
-
-
Pentylamine
-
-
Phe-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
Propylamine
-
-
SAK2
-
peptide vinyl sulfone, covalent inhibitor
Sodium deoxycholate
-
-
Trp-NH2
-
inhibition of the reaction with Gly-Gly-ethyl ester or Gly-L-Phe-NH2
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
beta-mercaptoethylamine
-
SH compound required, efficient activator at 25 mM
dithioerythritol
DL-homocysteine
-
SH compound required, efficient activator at 25 mM
GSH
-
SH compound required, efficient activator at 25 mM
mercaptoethanol
-
SH compound required, efficient activator at 25 mM
mercaptoethylamine
-
activates
Phenol
-
activates reaction with Gly-Gly-ethyl ester and Gly-Gly-NH2, no effect on reaction with Gly-Phe-NH2
Pyridine
-
activates reaction with Gly-Gly-ethyl ester and Gly-Gly-NH2, no effect on reaction with Gly-Phe-NH2
sulfhydryl activators
-
required
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.31
alpha-Asp-Arg-beta-naphthylamide
-
-
0.44
Asn1-angiotensin II
-
-
0.027
glucagon
-
removal of His-Ser
0.1
Gly-Arg-2-naphthylamide
-
-
0.14
Gly-Arg-4-methoxy-beta-naphthylamide
-
-
5.3
Gly-Gly-ethyl ester
-
-
4
Gly-Leu-ethyl ester
-
-
1.5
Gly-Phe-ethyl ester
-
-
1.3
Gly-Phe-methyl ester
-
-
0.63 - 1.5
Gly-Trp-methyl ester
6
Gly-Tyr-NH2
-
-
19
glycyl-(beta-phenyl)L-lactic acid methyl ester
-
-
0.022
His-Ser-2-naphthylamide
-
-
0.34
Ile5-angiotensin II
-
-
6.4 - 17
L-Ser-L-Tyr-7-amido-4-methylcoumarin
61
sarcosyl-Phe-ethyl ester
-
-
-
2.5
Val-Leu-NH2
-
-
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
87
Asn1-angiotensin II
Rattus norvegicus
-
removal of the first dipeptide
81
glucagon
Rattus norvegicus
-
removal of His-Ser
74
Gly-Gly-ethyl ester
Bos taurus
-
-
126
Gly-Leu-ethyl ester
Bos taurus
-
-
98
Gly-Phe-ethyl ester
Bos taurus
-
-
61
Gly-Phe-methyl ester
Bos taurus
-
-
293
Gly-Trp-methyl ester
Bos taurus
-
-
90
Gly-Tyr-ethyl ester
Bos taurus
-
-
210
glycyl-(beta-phenyl)L-lactic acid methyl ester
Bos taurus
-
-
116
His-Ser-2-naphthylamide
Rattus norvegicus
-
-
87
Ile5-angiotensin II
Rattus norvegicus
-
removal of the first dipeptide
157
sarcosyl-Phe-ethyl ester
Bos taurus
-
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000015
(2S)-2-amino-N-[(1S)-2-(biphenyl-4-yl)-1-cyanoethyl]butanamide
-
-
0.000045
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(m-fluorophenyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
-
-
1.563
Gly-DELTA(Z)Phe-AlaPO(OEt)2-trifluoroacetic acid
-
at 37C in acetate buffer (pH 5) containing NaCl (10 mM)
0.05
Gly-DELTAZPhe-Gly-DELTAEPhe-Gly
-
at 37C in acetate buffer (pH 5) containing NaCl (10 mM)
0.173
Gly-DELTAZPhe-Gly-DELTAEPhe-Gly-OMe
-
at 37C in acetate buffer (pH 5) containing NaCl (10 mM)
0.122
Gly-DELTAZPhe-Gly-DELTAEPhe-Phe
-
at 37C in acetate buffer (pH 5) containing NaCl (10 mM)
0.324
Gly-DELTAZPhe-Gly-DELTAEPhe-Phe-OMe
-
at 37C in acetate buffer (pH 5) containing NaCl (10 mM)
0.0000002
N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-norvalinamide
-
-
0.0018
N2-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-leucinamide
-
-
0.681
tert-butyloxycarbonyl-Gly-DELTA(Z)Phe-AbuPO(OMe)2
-
at 37C in acetate buffer (pH 5) containing NaCl (10 mM)
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
(2S)-2-amino-N-[(1S)-2-(biphenyl-4-yl)-1-cyanoethyl]butanamide
Homo sapiens
-
-
0.0000017
(4R)-N-[(1S)-1-cyano-2-phenylethyl]-4-(methylsulfanyl)-4-phenyl-L-prolinamide
Homo sapiens
-
-
0.0000082
(4R)-N-[(1S)-1-cyano-2-phenylethyl]-4-fluoro-4-phenyl-L-prolinamide
Homo sapiens
-
-
0.000495
(4S)-4-chloro-N-[(1S)-1-cyano-2-phenylethyl]-L-prolinamide
Homo sapiens
-
-
0.000336
(4S)-N-(1-cyanocyclopropyl)-4-(methylsulfanyl)-L-prolinamide
Homo sapiens
-
-
0.00088
(4S)-N-(1-cyanocyclopropyl)-4-fluoro-L-prolinamide
Homo sapiens
-
-
0.000192
(4S)-N-[(1R,2S)-2-benzyl-1-cyanocyclopropyl]-4-(methylsulfanyl)-L-prolinamide
Homo sapiens
-
-
0.000523
(4S)-N-[(1R,2S)-2-benzyl-1-cyanocyclopropyl]-4-fluoro-L-prolinamide
Homo sapiens
-
-
0.000271
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-(ethylsulfanyl)-L-prolinamide
Homo sapiens
-
-
0.000029
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-(methylsulfanyl)-L-prolinamide
Homo sapiens
-
-
0.000133
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-fluoro-L-prolinamide
Homo sapiens
-
-
0.001081
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-methoxy-L-prolinamide
Homo sapiens
-
-
0.002502
(4S)-N-[(1S)-1-cyano-2-phenylethyl]-4-methyl-L-prolinamide
Homo sapiens
-
-
0.000067
(4S)-N-[(1S,2R)-2-benzyl-1-cyanocyclopropyl]-4-(methylsulfanyl)-L-prolinamide
Homo sapiens
-
-
0.00025
(4S)-N-[(1S,2R)-2-benzyl-1-cyanocyclopropyl]-4-fluoro-L-prolinamide
Homo sapiens
-
-
0.002193
(4S)-N-[(1S,2S)-1-cyano-2-phenylcyclopropyl]-4-fluoro-L-prolinamide
Homo sapiens
-
-
0.000019
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(2-naphthyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
Homo sapiens
-
IC50: 19 nM
0.000022
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(benzo[b]-thiophen-3-yl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
Homo sapiens
-
IC50: 22 nM
0.000021
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(indol-3-yl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
Homo sapiens
-
IC50: 21 nM
0.000031
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(m-fluorophenyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
Homo sapiens
-
IC50: 31 nM, competitive inhibition, selective for DPP I over other cysteine and serine proteases, noncytotoxic
0.000039
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(m-methoxyphenyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
Homo sapiens
-
IC50: 39 nM
0.000045
1-(2S-2-aminobutanoyl)-4-(2S-N-(2S-3-(p-chlorophenyl)propan-2-yl-amide)4-phenylbutan-2-yl-amide)semicarbazide
Homo sapiens
-
IC50: 45 nM
0.000186
3-[4-(1-amino-1-cyclopentylethyl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.074
3-[4-(1-amino-2-phenylethyl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.00056
3-[4-(1-aminocyclohexyl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.011
3-[4-(1-aminocyclohexyl)-1H-1,2,3-triazol-1-yl]-2-oxoheptyl 2,6-dimethylbenzoate
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.0007
3-[4-(1-aminoethyl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.00041
3-[4-(2-aminopentan-2-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.0011
3-[4-(2-aminopropan-2-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.00042
3-[4-(2-methylpyrrolidin-2-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.00041
3-[4-(3-aminopentan-3-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.0007
3-[4-(4-aminoheptan-4-yl)-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.000019
3-[4-[(1R)-1-amino-1-cyclopentylethyl]-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.0007
3-[4-[(1S)-1-amino-1-cyclohexylethyl]-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.014
3-[4-[(1S)-1-amino-1-cyclopentylethyl]-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.0077
3-[4-[4-(methylamino)heptan-4-yl]-1H-1,2,3-triazol-1-yl]-1-(2,3,5,6-tetrafluorophenoxy)heptan-2-one
Plasmodium falciparum
-
determined after 30 min incubation of parasite lysate with 5 nM to 0.1 mM inhibitor, pH not specified in the publication, 25C
0.000063
N-(1-cyano-2,2-dimethylcyclopropyl)-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.00123
N-(1-cyano-2-phenylcyclopropyl)-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.001832
N-(1-cyanocyclobutyl)-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.000012
N-(1-cyanocyclopropyl)-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.001419
N-(2-cyanopropan-2-yl)-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.000017
N-(cyanomethyl)-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.0001
N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-norvalinamide
Homo sapiens
-
-
0.000014
N-[(1R,2R)-1-cyano-2-phenylcyclopropyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.0000038
N-[(1S)-1-cyano-2,2-diphenylethyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.0000058
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.0002
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-L-methioninamide
Homo sapiens
-
-
0.00033
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-L-valinamide
Homo sapiens
-
-
0.00063
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-S-ethyl-L-cysteinamide
Homo sapiens
-
-
0.00158
N-[(1S)-1-cyano-2-(1H-indol-3-yl)ethyl]-S-ethyl-L-homocysteinamide
Homo sapiens
-
-
0.0000003
N-[(1S)-1-cyano-2-(5-phenylthiophen-2-yl)ethyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.000001
N-[(1S)-1-cyano-2-(naphthalen-2-yl)ethyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.0000051
N-[(1S)-1-cyano-2-cyclohexylethyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.000215
N-[(1S)-1-cyano-2-methylpropyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.0000009
N-[(1S)-1-cyano-2-phenylethyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.004806
N-[(1S)-1-cyano-2-phenylethyl]-L-prolinamide
Homo sapiens
-
-
0.002545
N-[(1S)-1-cyano-2-[4-(methylsulfanyl)phenyl]ethyl]-L-prolinamide
Homo sapiens
-
-
0.000011
N-[(1S)-1-cyano-3-phenylpropyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.0000007
N-[(1S)-2-(1,3-benzothiazol-2-yl)-1-cyanoethyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.000017
N-[(1S,2S)-1-cyano-2-phenylcyclopropyl]-3-thiophen-2-yl-L-alaninamide
Homo sapiens
-
-
0.011
N-[2-(1H-indol-3-yl)ethyl]-L-methioninamide
Homo sapiens
-
-
0.004
N2-(morpholin-4-ylcarbonyl)-N-[(1E,3S)-5-phenyl-1-(phenylsulfonyl)pent-1-en-3-yl]-L-leucinamide
Homo sapiens
-
-
additional information
N-[(1S)-1-cyano-2,2-dimethylpropyl]-3-thiophen-2-yl-L-alaninamide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.019
-
-
18.6
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6
-
Asn1-angiotensin II or Ile5-angiotensin II as substrate
5.2
-
His-Ser-beta-naphthylamide as substrate
6.3
-
removal of His-Ser from glucagon
7.2 - 7.8
-
-
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7.5
-
50% of maximal activity at pH 4.0 and at pH 7.5
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.7
calculated from amino acid sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
secretion of active enzyme is stimulated by thyrotropin, insulin, and/or somatostatin
Manually annotated by BRENDA team
-
very low cathepsin C activity in culture medium of untreated keratinocytes, suggesting that the constitutive exocytosis of lysosomes is weak in keratinocytes. Treatment with 0.03 mM ionomycin for 50 min induces the release of about 20% of cathepsin C activity. Incubation of keratinocytes for 30 min with 1 mM calcium and 0.01 mM ionomycin causes a significant release of cathepsin C activity
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
-
cathepsin C activity in monocyte derived dendritic cells (ex vivo) decreases dramatically as they mature
Manually annotated by BRENDA team
-
gene for cathepsin C is upregulated compared to Barrett's esophagus
Manually annotated by BRENDA team
-
activity of dipeptidyl-peptidase I increases significantly as compared to the normal esophageal mucosa
Manually annotated by BRENDA team
-
epithelium from normal control Barrett's esophagus
Manually annotated by BRENDA team
-
activity of dipeptidyl-peptidase I increases significantly as compared to the normal gastric mucosa
Manually annotated by BRENDA team
-
activity of dipeptidyl-peptidase I increases significantly as compared to the normal gastric mucosa
Manually annotated by BRENDA team
-
decrease in enzyme activity compared to normal kidney
Manually annotated by BRENDA team
-
high activity
Manually annotated by BRENDA team
-
cytotoxic
Manually annotated by BRENDA team
additional information
-
almost similar level of IgG, IgM, and IgA antibodies to DPP I and CD13 in patients with mixed connective tissue disease and autism, but not in controls
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
in B-lymphoblastoid cells cathepsin is distrubuted between late endosomes and lysosomes
Manually annotated by BRENDA team
-
trafficking route for the enzyme through the parasitophorous vacuole to the food vacuole
Manually annotated by BRENDA team
additional information
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50000
calculated from amino acid sequence
51000
-
Western blot
100000
-
gel filtration
150000
-
gel filtration
180000
-
gel filtration
200000
-
gel filtration
210000
additional information
-
-
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
-
the proenzyme has a MW of 77400 Da after signal peptide cleavage
side-chain modification
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 8
-
stable
36145
4.6
-
stable up to 50C
36145
5
-
90 min, about 10% loss of activity
36135
5.5
-
stable up to 60C
36145
8
-
90 min, about 75% loss of activity
36135
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
immobilized enzyme, stable up to
60
-
at pH 5.5, stable up to
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, about 50% loss of activity after 4 days and 5 assay cycles, immobilized enzyme
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15C, stable
-
4C, stable for months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
11.1% yield after affinity chromatography on Ni-chelating resin
-
protein A affinity chromatography and Superdex 200 gel filtration
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
cathepsin C propeptide-green fluorescence protein chimera protein is expressed in Caco-2 cells, overexpression of cathepsin C propeptide does not affect cathepsin C activity in Caco-2 cells because the propeptide itself does not have the catalytic domain of cathepsin C
-
expressed in CHO cells
-
expressed in DPPI-deficient mice
-
expression in Plasmodium berghei
expression in the High Five insect cell line
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
highest expression at maturation
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
R272P
-
missense mutation found in patients affected with classical features of Papillon-Lefevre syndrome
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
medicine
-
mast cell DPPI harms the septic host. DPPI is a novel potential therapeutic target for treatment of sepsis
nutrition
-
muscle DPP I may be a key enzyme responsible for the generation of dipeptides in Jinhua ham processing
synthesis
-
specific and efficient method for complete removal of polyhistidine purification tags from the N-termini of target proteins