Information on EC 3.4.13.5 - Xaa-methyl-His dipeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.13.5
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RECOMMENDED NAME
GeneOntology No.
Xaa-methyl-His dipeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of anserine (beta-alanyl-/-Npi-methyl-L-histidine), carnosine, homocarnosine, glycyl-/-leucine and other dipeptides with broad specificity.
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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dipeptide hydrolase
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-Alanine metabolism
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Histidine metabolism
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CAS REGISTRY NUMBER
COMMENTARY hide
37289-11-5
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9027-38-7
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-His + H2O
Ala + His
show the reaction diagram
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-
-
?
Ala-Pro + H2O
Ala + Pro
show the reaction diagram
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-
-
?
anserine + H2O
beta-Ala + 1-methylhistidine
show the reaction diagram
anserine + H2O
beta-alanine + 3-methyl-L-histidine
show the reaction diagram
-
-
-
?
carnosine + H2O
?
show the reaction diagram
carnosine + H2O
beta-alanine + L-His
show the reaction diagram
-
-
-
?
Gly-Gly + H2O
2 Gly
show the reaction diagram
306% of the activity with L-Ala-L-His
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-
?
Gly-Gly + H2O
Gly
show the reaction diagram
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-
-
?
Gly-Gly + H2O
Gly + Gly
show the reaction diagram
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385% of the activity with N-acetyl-L-His
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-
?
Gly-His + H2O
Gly + His
show the reaction diagram
Gly-L-His + H2O
Gly + L-His
show the reaction diagram
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110% of the activity with N-acetyl-L-His, brain enzyme
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-
?
Gly-L-Leu + H2O
Gly + L-Leu
show the reaction diagram
Gly-Leu + H2O
Gly + Leu
show the reaction diagram
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-
-
-
?
His-Gly + H2O
His + Gly
show the reaction diagram
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-
-
?
homocarnosine + H2O
4-aminobutyric acid + L-His
show the reaction diagram
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-
-
?
homocarnosine + H2O
?
show the reaction diagram
L-Ala-L-His + H2O
L-Ala + L-His
show the reaction diagram
L-Leu-Gly + H2O
L-Leu + Gly
show the reaction diagram
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101% of the activity with N-acetyl-L-His, brain enzyme
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-
?
L-Pro-Gly + H2O
L-Pro + Gly
show the reaction diagram
180% of the activity with L-Ala-L-His
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?
Leu-Gly + H2O
Leu + Gly
show the reaction diagram
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-
-
?
N-acetyl-L-Glu + H2O
L-Glu + acetate
show the reaction diagram
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23% of the activity with N-acetyl-L-His, brain enzyme
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-
?
N-acetyl-L-His + H2O
His + acetate
show the reaction diagram
N-acetyl-L-Leu + H2O
L-Leu + acetate
show the reaction diagram
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110% of the activity with N-acetyl-L-His, brain enzyme
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-
?
N-acetyl-L-methionine + H2O
L-Met + acetate
show the reaction diagram
N-acetyl-L-Trp + H2O
L-Trp + acetate
show the reaction diagram
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30% of the activity with N-acetyl-L-His, brain enzyme
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-
?
Nalpha-acetyl-L-His + H2O
His + acetate
show the reaction diagram
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-
-
?
Nalpha-chloro-acetyl-L-Leu + H2O
chloroacetate + Leu
show the reaction diagram
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-
-
?
Nalpha-chloroacetyl-L-Leu + H2O
chloroacetic acid + L-Leu
show the reaction diagram
501% of the activity with L-Ala-L-His
-
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?
Nalpha-chloroacetyl-L-Leu + H2O
L-Leu + chloroacetate
show the reaction diagram
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1235% of the activity with N-acetyl-L-His, brain enzyme
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?
Pro-Gly + H2O
Pro + Gly
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
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enzyme may be required to degrade N-acetyl-L-histidine after formation of a transient capsular complex
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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2-mercaptoethanol
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Ag+
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1 mM, 90% inhibition
bestatin
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0.01 mM, 15% inhibition of the enzyme from eye, 1% inhibition of the enzyme from brain
Chloropromazine
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Co2+
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1 mM, 10% inhibition
Cys
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90% inhibition at 1 mM, 40% inhibition at 0.1 mM
dithiothreitol
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Fe3+
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1 mM, 94% inhibition
Mn2+
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1 mM, 30% inhibition
phenylmethylsulfonyl fluoride
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phosphate
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Trifluorperazine
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.03
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brain
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
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anserine
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
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less than 10% of maximal activity at pH 5.0 and 9.0
5.2 - 8.5
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50% of maximal activity at pH 5.2 and at pH 8.5
6 - 8
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pH 6.0: about 35% of maximal activity, pH 8.0: about 55% of maximal activity
6.5 - 7.8
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activity is sharply reduced below pH 6.5 and above pH 7.8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 60
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10C: about 50% of maximal activity, brain and eye enzyme, 60C, about 40% of maximal activity, brain enzyme, 60C: about 90% of maximal activity, eye enzyme
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3
calculated from sequence
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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trace amounts
Manually annotated by BRENDA team
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moderate activity
Manually annotated by BRENDA team
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cortex, concentrated in the capsule at levels up to 3 mg/ml
Manually annotated by BRENDA team
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trace amounts
Manually annotated by BRENDA team
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trace amounts
Manually annotated by BRENDA team
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trace amounts
Manually annotated by BRENDA team
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very rich source
Manually annotated by BRENDA team
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trace amounts
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52661
x * 52661, calculated
53311
x * 53311, calculated from sequence
55000
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2 * 55000, SDS-PAGE
83000
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gel filtration
120000
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 55000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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unstable at
35025
4.7 - 6.4
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maximal stability at 30C, pH 4.7-6.4, in presence of ZnSO4
35025
5.2 - 8.1
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maximal stability
36081
8.5
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unstable at
35025
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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maximal stability at 30C, pH 4.7-6.4, in presence of ZnSO4
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
from skeletal muscle
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE