Information on EC 3.4.13.17 - non-stereospecific dipeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.13.17
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RECOMMENDED NAME
GeneOntology No.
non-stereospecific dipeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of dipeptides containing either D- or L-amino acids or both
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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CAS REGISTRY NUMBER
COMMENTARY hide
90371-43-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-Ala-D-Ala + H2O
D-Ala + D-Ala
show the reaction diagram
-
3-20% hydrolysis, depending on reaction time
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?
D-Ala-Gly + H2O
D-Ala + Gly
show the reaction diagram
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15-100% hydrolysis, depending on reaction time
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?
D-Ala-L-Ala + H2O
D-Ala + L-Ala
show the reaction diagram
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1-10% hydrolysis, depending on reaction time
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?
D-amino acid peptide + H2O
?
show the reaction diagram
D-Leu-D-Leu + H2O
D-Leu + D-Leu
show the reaction diagram
-
75-100% hydrolysis, depending on reaction time
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?
D-Leu-Gly-Gly + H2O
D-Leu + Gly-Gly
show the reaction diagram
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30-100% hydrolysis, depending on reaction time
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?
D-Leu-Leu + H2O
D-Leu + Leu
show the reaction diagram
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70-100% hydrolysis, depending on reaction time
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?
Gly-D-Ala + H2O
Gly + D-Ala
show the reaction diagram
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80-100% hydrolysis, depending on reaction time
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?
Gly-D-Asp + H2O
Gly + D-Asp
show the reaction diagram
-
30-100% hydrolysis, depending on reaction time
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?
Gly-D-Leu + H2O
Gly + D-Leu
show the reaction diagram
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90-100% hydrolysis, depending on reaction time
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?
Gly-D-Phe + H2O
Gly + D-Phe
show the reaction diagram
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10-50% hydrolysis, depending on reaction time
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?
Gly-D-Ser + H2O
Gly + D-Ser
show the reaction diagram
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30-100% hydrolysis, depending on reaction time
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?
Gly-Gly-D-Leu + H2O
Gly-Gly + D-Leu
show the reaction diagram
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2-20% hydrolysis, depending on reaction time
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?
L-Ala-D-Ala + H2O
L-Ala + Ala
show the reaction diagram
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100% hydrolysis
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?
L-Leu-D-Leu + H2O
Leu + D-Leu
show the reaction diagram
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2-15% hydrolysis, depending on reaction time
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?
L-Tyr-D-Ala-Gly-L-Phe-L-Met-NH2 + H2O
L-Tyr + D-Ala-Gly-L-Phe-L-Met-NH2
show the reaction diagram
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4-40% hydrolysis, depending on reaction time
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?
L-Tyr-D-Arg + H2O
L-Tyr + D-Arg
show the reaction diagram
-
2-20% hydrolysis, depending on reaction time
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?
additional information
?
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also hydrolysis of L-peptides and enkephalins, no hydrolysis of peptides with substituted amino-terminus
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-amino acid peptide + H2O
?
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
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50 mM, 5-10% inhibition, no inhibition up to 1 mM
CdCl2
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50 mM, 5-10% inhibition, no inhibition up to 1 mM
Pb(CH3COO)2
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50 mM, 5-10% inhibition, no inhibition up to 1 mM
additional information
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not inhibitory are 1 mM of MnCl2, MgCl2, ZnCl2, CuCl2 or 100 mM of NaCl, KCl, Na2SO4 or 50 mM of EDTA, guanidine chloride or 3 M of urea
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.4
D-Leu-D-Leu
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5.2
Gly-D-Ala
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7.7
Gly-L-Ala
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2.5
L-Ala-D-Ala
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2.8
L-Ala-L-Ala
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8.6
L-Leu-L-Leu
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23.3
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substrate Gly-D-Ala
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 8.8
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
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1 * 106000 + 1 * 34000, SDS-PAGE
106000
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1 * 106000 + 1 * 34000, SDS-PAGE
140000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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1 * 106000 + 1 * 34000, SDS-PAGE
additional information
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amino acid analysis: high contributions of Asp, Glu, Gly, Val
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
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pH 8.0, stable for 5 days
50
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pH 8.0, 5 min, 50% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 0.05 M phosphate buffer, pH 7.0, 50% glycerol, 6 months, little loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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improved method for hydrolyzing proteins and peptides without inducing racemization and for determining their true D-amino acid content