Information on EC 3.4.13.12 - Met-Xaa dipeptidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.13.12
-
RECOMMENDED NAME
GeneOntology No.
Met-Xaa dipeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of Met-/-Xaa dipeptides.
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
-
-
dipeptide hydrolase
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Cysteine and methionine metabolism
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-
CAS REGISTRY NUMBER
COMMENTARY hide
37341-91-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-Ala + H2O
Ala + Ala
show the reaction diagram
-
-
-
ir
Ala-Leu + H2O
Ala + Leu
show the reaction diagram
-
-
-
ir
Ala-Met + H2O
Ala + Met
show the reaction diagram
ethionyl-Ala + H2O
ethionine + Ala
show the reaction diagram
-
-
-
ir
Gly-Gly + H2O
Gly + Gly
show the reaction diagram
-
-
-
ir
Leu-Ala + H2O
Leu + Ala
show the reaction diagram
Met-Ala + H2O
Met + Ala
show the reaction diagram
Met-amino acid + H2O
?
show the reaction diagram
Met-Glu + H2O
Met + Glu
show the reaction diagram
-
cleaved at about 10% of the maximal rate
-
-
?
Met-Gly + H2O
Met + Gly
show the reaction diagram
Met-Ile + H2O
Met + Ile
show the reaction diagram
Met-Leu + H2O
Met + Leu
show the reaction diagram
Met-Met + H2O
Met + Met
show the reaction diagram
-
-
-
ir
Met-Ser + H2O
Met + Ser
show the reaction diagram
Met-Thr + H2O
Met + Thr
show the reaction diagram
Met-Val + H2O
Met + Val
show the reaction diagram
norleucyl-Ala + H2O
norleucine + Ala
show the reaction diagram
-
-
-
ir
Ser-Ala + H2O
Ser + Ala
show the reaction diagram
Thr-Ala + H2O
Thr + Ala
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Met-amino acid + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
52% of activity with manganese
Fe2+
-
21% of activity with manganese
Zn2+
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20% of activity with manganese
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
iodoacetate
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100% inhibition at 10 mM, 10 mM mercaptoethanol restores activity
iodoacetic acid
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p-chloromercuribenzoate
PCMB
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inhibition is reversed by 2-mercaptoethanol
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25 - 0.31
Met-Ala
0.42 - 1.02
Met-Ile
0.45
Met-Leu
-
-
0.57 - 0.6
Met-Ser
0.53
Met-Thr
-
-
0.13
Met-Val
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8 - 9
8.5
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substrate Met-Ala
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
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2 * 47000, SDS-PAGE
93000 - 95000
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gel filtration
94000
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gel filtration
110000 - 114000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
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2 * 47000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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below, 30 min, Mn2+ protects against heat inactivation
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
generally unstable
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unstable in absence of metal ions and reducing agents. Mn2+ is the best cation for maintaining stability
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, 10 mM imidazole buffer, pH 7.2, 2 mg/ml bovine serum albumin, 30 days, 50% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE