Information on EC 3.4.11.B3 - Sulfolobus solfataricus aminopeptidase

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The expected taxonomic range for this enzyme is: Sulfolobus solfataricus

EC NUMBER
COMMENTARY hide
3.4.11.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Sulfolobus solfataricus aminopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Preferentially hydrolyzes Xaa-/-Yaa-, in which Xaa is preferentially a non-polar amino acid, L-Ala or L-Leu. When Xaa is L-Phe or a polar amino acid residue like L-Gly or a basic amino acid residue like L-Lys and L-Arg the rates of hydrolysis are lower
show the reaction diagram
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glycyl 4-nitroanilide + H2O
glycine + 4-nitroaniline
show the reaction diagram
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11% of the activity compared to L-Leu-4-nitroanilide
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-
?
L-alanyl 4-nitroanilide + H2O
L-alanine + 4-nitroaniline
show the reaction diagram
L-arginyl 4-nitroanilide + H2O
L-arginine + 4-nitroaniline
show the reaction diagram
L-leucyl 4-nitroanilide + H2O
L-leucine + 4-nitroaniline
show the reaction diagram
L-lysyl 4-nitroanilide + H2O
L-lysine + 4-nitroaniline
show the reaction diagram
L-methionyl 4-nitroanilide + H2O
L-methionine + 4-nitroaniline
show the reaction diagram
about 70% of the activity compared to L-leucyl 4-nitroanilide
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-
?
L-phenylalanyl 4-nitroanilide + H2O
L-phenylalanine + 4-nitroaniline
show the reaction diagram
L-prolyl 4-nitroanilide + H2O
L-proline + 4-nitroaniline
show the reaction diagram
about 20% of the activity compared to L-leucyl 4-nitroanilide
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?
additional information
?
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no activity with N-acetyl-L-phenylalanine, N-acetyl-L-leucine, N-benzoyl-L-arginine
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
-
1 mM, restores enzyme activity after inhibition with EDTA
Mn2+
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1 mM, restores enzyme activity after inhibition with EDTA
Zn2+
zinc-dependent enzyme
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetic acid
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1%, 86% inhibition
acetonitrile
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10%, 26% inhibition
bestatin
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1 mM, 97% inhibition
EDTA
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1 mM, complete inhibition
ethanol
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10%, complete inhibition
mercaptoethanol
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1%, 56% inhibition
SDS
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0.1%, complete inhibition
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
L-alanyl 4-nitroanilide
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pH 7.0, 70°C
0.1
L-leucyl 4-nitroanilide
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pH 7.0, 70°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.225
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pH 7.0, 70°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7.5
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pH 5.5: about 45% of maximal activity, pH 7.5: about 65% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75
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adition of 0.05 mM each of Co2+, Mn2+, Mg2+, Ca2+ and Zn2+ has no effect on the temperature optimum. At higher concentrations of divalent metal ions a decrease of activity occurs
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60 - 90
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60°C: about 60% of maximal activity, 90°C: about 35% of maximal activity
80 - 95
80°C: optimum, 95°C: 40% of maximal activity, no activity below 30°C
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.4
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isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80000
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4 * 80000, SDS-PAGE
90000
x * 90000, SDS-PAGE
320000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 90000, SDS-PAGE
homotetramer
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4 * 80000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 9
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14 h, at least 70% residual activity
718953
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75
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15 min, activity decreases to 10% in absence of Co2+, in presence of 0.05 mM the remaining activity is 76%
85
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15 min, complete loss of activity in absence of Co2+, in presence of 0.05 mM the remaining activity is 60%
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE