Information on EC 3.4.11.7 - glutamyl aminopeptidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.4.11.7
-
RECOMMENDED NAME
GeneOntology No.
glutamyl aminopeptidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of N-terminal glutamate (and to a lesser extent aspartate) from a peptide
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of peptide bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
alliin metabolism
-
-
methiin metabolism
-
-
propanethial S-oxide biosynthesis
-
-
CAS REGISTRY NUMBER
COMMENTARY hide
9074-83-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Merril cv. Kegon
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-
Manually annotated by BRENDA team
strain DBPZ1009, DBPZ1010, DBPZ1017, DBPZ1018, DBPZ1020, DBPZ1024
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-
Manually annotated by BRENDA team
strain DBPZ1009, DBPZ1010, DBPZ1017, DBPZ1018, DBPZ1020, DBPZ1024
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-
Manually annotated by BRENDA team
strain DBPZ0997, DBPZ1001, DBPZ1004, DBPZ1013, DBPZ1027
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-
Manually annotated by BRENDA team
strain DBPZ0997, DBPZ1001, DBPZ1004, DBPZ1013, DBPZ1027
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-
Manually annotated by BRENDA team
strain DBPZ0984
-
-
Manually annotated by BRENDA team
strain DBPZ0984
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-
Manually annotated by BRENDA team
strain DBPZ0987, DBPZ0988, DBPZ0989, DBPZ0990, DBPZ0991, DBPZ0992, DBPZ0993, DBPZ0998, DBPZ0999, DBPZ1002, DBPZ1003, DBPZ1008, DBPZ1011, DBPZ1012, DBPZ1014, DBPZ1015, DBPZ1019, DBPZ1021, DBPZ1023, DBPZ1025
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-
Manually annotated by BRENDA team
strain DBPZ0987, DBPZ0988, DBPZ0989, DBPZ0990, DBPZ0991, DBPZ0992, DBPZ0993, DBPZ0998, DBPZ0999, DBPZ1002, DBPZ1003, DBPZ1008, DBPZ1011, DBPZ1012, DBPZ1014, DBPZ1015, DBPZ1019, DBPZ1021, DBPZ1023, DBPZ1025
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-
Manually annotated by BRENDA team
strain DBPZ0985, DBPZ0986, DBPZ0995, DBPZ1005, DBPZ1016, DBPZ1026, DBPZ1028
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-
Manually annotated by BRENDA team
Balb/c mice
-
-
Manually annotated by BRENDA team
rainbow trout
-
-
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
PAO1
-
-
Manually annotated by BRENDA team
gilthead sea bream
-
-
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
DBPZ1006, DBPZ1007
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-L-Asp-2-naphthylamide + H2O
alpha-L-Asp + 2-naphthylamide
show the reaction diagram
-
-
-
-
?
alpha-L-Asp-Val-angiotensin II + H2O
Asp + Val-angiotensin II
show the reaction diagram
-
-
-
-
?
alpha-L-Glu-2-naphthylamide + H2O
alpha-L-Glu + 2-naphthylamide
show the reaction diagram
-
-
-
-
?
alpha-L-Glu-beta-naphthylamide + H2O
?
show the reaction diagram
-
-
-
-
?
alpha-L-Glu-beta-naphthylamide + H2O
alpha-L-Glu + beta-naphthylamide
show the reaction diagram
-
-
-
-
?
Angiotensin I + H2O
?
show the reaction diagram
angiotensin I + H2O
Asp + Arg-Val-Tyr-Ile-His-Pro-Phe-His-Leu
show the reaction diagram
-
-
-
-
?
angiotensin II
Asp + Arg-Val-Tyr-Ile-His-Pro-Phe
show the reaction diagram
Angiotensin II + H2O
?
show the reaction diagram
angiotensin II + H2O
angiotensin III + Arg
show the reaction diagram
angiotensin II + H2O
Asp + angiotensin III
show the reaction diagram
angiotensin II + H2O
aspartic acid + angiotensin III
show the reaction diagram
angiotensin II + H2O
L-Asp + angiotensin III
show the reaction diagram
-
-
-
-
?
angiotensin II + H2O
L-aspartic acid + angiotensin III
show the reaction diagram
angiotensin III + H2O
? + angiotensin IV
show the reaction diagram
-
i.e. Tyr-Arg-Val-Tyr-Ile-His-Pro-Phe, low activity in absence of Ca2+
-
-
?
angiotensin III + H2O
angiotensin IV + Arg
show the reaction diagram
Asp-4-methylcoumaryl-7-amide + H2O
Asp + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity in absence and presence of Ca2+
-
-
?
Asp-4-nitroanilide + H2O
Asp + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
Asp-7-amido-4-methylcoumarin + H2O
Asp + 7-amino-4-methylcoumarin
show the reaction diagram
assay for measuring aspartyl aminopeptidase activity
-
-
?
Asp-Ala + H2O
Asp + Ala
show the reaction diagram
Asp-beta-naphthylamide + H2O
Asp + naphthylamine
show the reaction diagram
Asp-Phe-NH2 + H2O
Asp + Phe + NH3
show the reaction diagram
-
-
-
-
?
aspartame + H2O
L-Asp + L-Phe methyl ester
show the reaction diagram
-
-
-
-
?
cholecystokinin-8 + H2O
?
show the reaction diagram
cholecystokinin-8 + H2O
Asp + YMGWMDF-NH2
show the reaction diagram
-
i.e. CCK8 or DYMGWMDF-NH2
-
-
?
cholecystokinin-8 + H2O
L-Asp + YMGWMDF-NH2
show the reaction diagram
-
-
-
-
?
chromogranin A + H2O
?
show the reaction diagram
-
-
-
-
?
chromogranin A + H2O
Glu + EEEEMAVVPQGLFRG-NH2
show the reaction diagram
-
i.e. EEEEEMAVVPQGLFRG-NH2
-
-
?
Gln-4-methylcoumaryl-7-amide + H2O
Gln + 7-amino-4-methylcoumarin
show the reaction diagram
-
low activity in absence and presence of Ca2+
-
-
?
Glu-2-naphthylamide + H2O
Glu + 2-naphthylamide
show the reaction diagram
-
-
-
-
?
Glu-2-naphthylamide + H2O
Glu + 2-naphthylamine
show the reaction diagram
Glu-4-methylcoumaryl-7-amide + H2O
Glu + 7-amino-4-methylcoumarin
show the reaction diagram
Glu-4-nitroanilide + H2O
Glu + 4-nitroaniline
show the reaction diagram
Glu-7-amido-4-methylcoumarin + H2O
Gln + 7-amino-4-methylcoumarin
show the reaction diagram
Glu-7-amido-4-methylcoumarin + H2O
Glu + 7-amino-4-methylcoumarin
show the reaction diagram
assay for measuring aspartyl aminopeptidase activity
-
-
?
Glu-Ala + H2O
Glu + Ala
show the reaction diagram
Glu-beta-naphthylamide + H2O
Glu + naphthylamine
show the reaction diagram
Glu-Glu + H2O
2 Glu
show the reaction diagram
-
many Glu-Xxx and Asp-Xxx dipeptides are substrates
-
-
?
Glu-p-nitroanilide + H2O
Glu + 4-nitroaniline
show the reaction diagram
Glu-p-nitroanilide + H2O
Glu + p-nitroaniline
show the reaction diagram
Glu-p-nitroanilide + H2O
glutamic acid + p-nitroaniline
show the reaction diagram
kallidin + H2O
?
show the reaction diagram
-
-
-
-
?
kallidin + H2O
Lys + bradykinin
show the reaction diagram
-
i.e. Lys-bradykinin or KRPPGFSPFR, the reaction is performed only in absence of Ca2+
i.e. RPPGFSPFR
-
?
L-Ala-4-methylcoumaryl-7-amide + H2O
L-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
L-Arg-2-naphthylamide + H2O
L-Arg + 2-naphthylamine
show the reaction diagram
L-Asp-peptide + H2O
L-Asp + peptide
show the reaction diagram
L-Glu-2-naphthylamide + H2O
L-Glu + 2-naphthylamine
show the reaction diagram
L-Glu-4-methylcoumaryl-7-amide + H2O
L-Glu + 7-amino-4-methylcoumarin
show the reaction diagram
L-Glu-7-amido-4-methylcoumarin + H2O
L-Glu + 7-amino-4-methylcoumarin
show the reaction diagram
L-Glu-p-nitroanilide + H2O
L-Glu + p-nitroaniline
show the reaction diagram
-
-
-
-
?
L-Glu-peptide + H2O
L-Glu + peptide
show the reaction diagram
L-Leu-4-methylcoumaryl-7-amide + H2O
L-Leu + 7-amino-4-methylcoumarin
show the reaction diagram
Leu-7-amido-4-methylcoumarin + H2O
Leu + 7-amino-4-methylcoumarin
show the reaction diagram
assay for measuring aspartyl aminopeptidase activity
-
-
?
milk proteins + H2O
?
show the reaction diagram
-
-
-
-
-
N-(alpha-L-Ala-L-Leu-L-Lys-Arg)-2-naphthylamide + H2O
?
show the reaction diagram
N-(alpha-L-aspartyl)-2-naphthylamide + H2O
L-aspartic acid + 2-naphthylamine
show the reaction diagram
N-(alpha-L-aspartylyl)-2-naphthylamide + H2O
L-aspartic acid + 2-naphthylamine
show the reaction diagram
N-(alpha-L-glutamyl)-2-naphthylamide + H2O
L-glutamic acid + 2-naphthylamine
show the reaction diagram
N-(alpha-L-glutamyl)-4-nitroanilide + H2O
L-glutamic acid + 4-nitroaniline
show the reaction diagram
N-(alpha-L-glutamyl)-beta-naphthylamide + H2O
L-glutamic acid + beta-naphthylamine
show the reaction diagram
N-Glu-L-Phe-4-nitroanilide + H2O
L-Glu + L-Phe-4-nitroanilide
show the reaction diagram
-
-
-
-
?
neurokinin B + H2O
?
show the reaction diagram
-
-
-
-
?
neurokinin B + H2O
Asp + MHDFFVGLM-NH2
show the reaction diagram
-
i.e. DMHDFFVGLM-NH2
-
-
?
Ser-Ala + H2O
Ser + Ala
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Angiotensin II + H2O
?
show the reaction diagram
-
-
-
-
-
angiotensin II + H2O
Asp + angiotensin III
show the reaction diagram
angiotensin II + H2O
aspartic acid + angiotensin III
show the reaction diagram
angiotensin III + H2O
angiotensin IV + Arg
show the reaction diagram
cholecystokinin-8 + H2O
?
show the reaction diagram
-
i.e. CCK8
-
-
?
chromogranin A + H2O
?
show the reaction diagram
-
-
-
-
?
milk proteins + H2O
?
show the reaction diagram
-
-
-
-
-
neurokinin B + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Zinc
-
zinc-dependent metalloprotease, activity requires the HEXXH zinc-binding motif
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(1-aminopropane-1,3-diyl)bis(phosphonic acid)
-
(3R)-3-amino-3-phosphonopropanoic acid
-
(R)-3-amino-4-mercaptobutane-1-sulfonic acid
-
i.e. EC33
(S)-3-amino-4-mercapto-butylsulfonic acid
(S)-3-amino-4-mercaptobutyl sulfonic acid
1,10-phenanthroline
2-amino-4-methylsulfonyl butane thiol
3-amino-3-[hydroxy(phenyl)phosphoryl]propanoic acid
-
3-amino-4-thio-butyl sulfonate
-
-
3-amino-4-thio-butyl sulfonic acid
-
EC33, specific and selective APA inhibitor
3-[(1-amino-2-carboxyethyl)(hydroxy)phosphoryl]-2-methylpropanoic acid
-
3-[[amino(carboxy)methyl](hydroxy)phosphoryl]-2-methylpropanoic acid
-
4,4'-dithio [bis[(3S)-3-aminobutyl sulfonic acid]]
-
i.e. RB150
4,4'-dithio[bis(3)-aminobutylsulfonic acid]
4,4'-dithio[bis(3-aminobutyl sulfonic acid)]
-
RB150, a prodrug of EC33
5-amino-5-phosphonopentanoic acid
-
Ala-PSI[PO2-CH2]-Leu-Ala
-
-
amastatin
amastin
-
3% residual activity at a concentration of 0.001 mM
-
amino(phosphono)acetic acid
-
amino[hydroxy(methyl)phosphoryl]acetic acid
-
angiotensin II
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+
Angiotensin III
-
competitive against Glu-4-methylcoumaryl-7-amide
angiotensin IV
aspartate hydroxamate
-
-
bestatin
bradykinin
-
-
captopril
cholecystokinin-8
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.024 mM in presence of Ca2+
chromogranin A
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.049 mM in presence of Ca2+
-
D-glutamate phosphonic acid
-
-
diisopropylfluorophosphate
-
-
dithiothreitol
DTT
-
32% activity at 0.1 mM
E64
-
slight inhibition at 1 mM
Glu-PSI[PO2-CH2]-Leu
-
-
Glu-PSI[PO2-CH2]-Leu-Ala
-
-
Glu-PSI[PO2-CH2]-Leu-Arg
-
-
Glu-PSI[PO2-CH2]-Leu-Asp
-
-
Glu-PSI[PO2-CH2]-Leu-Leu
-
-
glutamate phosphonate
glutamate phosphonic acid
-
-
glutamate thiol
-
-
glutamate-thiol
-
-
glutamine-thiol
-
-
kallidin
-
competitive against Glu-4-methylcoumaryl-7-amide
L-alpha-glutaryl-L-phenylalanine
-
-
L-aspartic acid
-
-
L-glutamate phosphinic acid
L-glutamate phosphonic acid
-
-
L-Glutamic acid
-
-
leupeptin
-
slight inhibition at 1 mM
lysine thiol
-
-
lysine-thiol
-
-
mercaptoethanol
-
-
Met-enkephalin
-
-
methionine thiol
Mg2+
-
inhibits the activity with Glu-substrate moderately
N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide
-
-
N-bromosuccinimide
-
-
Neurokinin A
-
-
neurokinin B
-
competitive against Glu-4-methylcoumaryl-7-amide,IC50 is 0.060 mM in presence of Ca2+
o-phenanthroline
p-hydroxymercuribenzoate
-
-
parathyroid hormone residues 69-84
-
-
-
phenylmethylsulfonyl fluoride
-
-
puromycin
-
-
RB150
Sodium azide
-
-
Substance P
-
-
ZnCl2
-
0% residual activity at a concentration of 1 mM
[Ag2(E-6-(hydroxyimino)ethyl-1,3,7-trimethyl-pteridine-2,4(1H,3H)-dione)2(CF3SO3)2(EtOH)]n x nEtOH
-
compound shows cytotoxicity against both U373-MG abd NB-69 cell lines. NB-69 cells need higher concentrations of silver complexes than U373-MG cells to obtain a 50% growth inhibition. All compounds tested show apoptotic effects, with U373-MG cells being more susceptible. The silver complexes tested also show a dose-dependent inhibitory effect on aminopeptidase A activity in NB-69 and U373-MG cells, although U373-MG cells are more sensitive
-
[Ag2(E-6-(hydroxyimino)ethyl-1,3,7-trimethyl-pteridine-2,4(1H,3H)-dione)2(ClO4)2]n
-
compound shows cytotoxicity against both U373-MG abd NB-69 cell lines. NB-69 cells need higher concentrations of silver complexes than U373-MG cells to obtain a 50% growth inhibition. All compounds tested show apoptotic effects, with U373-MG cells being more susceptible. The silver complexes tested also show a dose-dependent inhibitory effect on aminopeptidase A activity in NB-69 and U373-MG cells, although U373-MG cells are more sensitive
-
[Ag2(E-6-(hydroxyimino)ethyl-1,3,7-trimethyl-pteridine-2,4(1H,3H)-dione)2(NO3)2]n
-
compound shows cytotoxicity against both U373-MG abd NB-69 cell lines. NB-69 cells need higher concentrations of silver complexes than U373-MG cells to obtain a 50% growth inhibition. All compounds tested show apoptotic effects, with U373-MG cells being more susceptible. The silver complexes tested also show a dose-dependent inhibitory effect on aminopeptidase A activity in NB-69 and U373-MG cells, although U373-MG cells are more sensitive
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
-
-
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
-
-
[[(2R,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2R,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2R,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
-
[[(2R,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentane]-Ile-Asp-OH
-
-
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Ile-Asp-OH
-
-
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Tyr-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
-
-
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
-
-
[[(2S,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2S,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
[[(2S,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
-
[[(2S,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
-
additional information
-
inhibitory effects of peptide hormones on enzyme activity in presence or absence of Ca2+, overview, no or poor inhibition by 4-(amidinophenyl)-methanesulfonyl fluoride, pepstatin, and bestatin
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
BaCl2
-
319% residual activity at a concentration of 1 mM
CaCl2
-
473% residual activity at a concentration of 1 mM
MnCl2
-
181% residual activity at a concentration of 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.515 - 2.307
alpha-L-Asp-2-naphthylamide
0.243 - 2.687
alpha-L-Glu-2-naphthylamide
0.1 - 5.94
alpha-L-Glu-beta-naphthylamide
0.062 - 2.85
angiotensin II
1.4 - 2.3
Asp-4-methylcoumaryl-7-amide
0.3273 - 0.3848
Asp-7-amido-4-methylcoumarin
0.21 - 0.212
Asp-Ala
0.72 - 0.846
Asp-beta-naphthylamide
-
maternal and normal serum
1.21 - 3.25
cholecystokinin-8
0.6 - 7.1
Gln-4-methylcoumaryl-7-amide
0.4 - 1.2
Glu-4-methylcoumaryl-7-amide
0.1351 - 4.36
Glu-7-amido-4-methylcoumarin
0.28 - 0.283
Glu-Ala
0.031 - 1.2
Glu-beta-naphthylamide
0.87 - 2.05
Glu-p-nitroanilide
0.36 - 1.13
kallidin
0.159 - 2.034
L-Glu-2-naphthylamide
0.152 - 1.475
L-Glu-p-nitroanilide
1.84 - 4.94
neurokinin B
27.3
Ser-Ala
-
-
additional information
additional information
-
kinetics and substrate specificity in presence or absence of Ca2+
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14 - 102
alpha-L-Asp-2-naphthylamide
184 - 404
alpha-L-Glu-2-naphthylamide
1.8 - 1800
alpha-L-Glu-beta-naphthylamide
2.4 - 20
angiotensin II
2.8 - 12.2
Asp-4-methylcoumaryl-7-amide
0.05 - 0.354
Asp-7-amido-4-methylcoumarin
0.031 - 9.95
cholecystokinin-8
9.5 - 14.1
Gln-4-methylcoumaryl-7-amide
13.3 - 33.3
Glu-4-methylcoumaryl-7-amide
0.011 - 5.25
Glu-7-amido-4-methylcoumarin
0.7 - 1.55
kallidin
2 - 640
L-Glu-2-naphthylamide
295 - 720
L-Glu-p-nitroanilide
0.052 - 10.47
neurokinin B
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0044
(1-aminopropane-1,3-diyl)bis(phosphonic acid)
-
0.00034
(3R)-3-amino-3-phosphonopropanoic acid
-
0.00025
(S)-3-amino-4-mercaptobutyl sulfonic acid
-
-
2
3-amino-3-[hydroxy(phenyl)phosphoryl]propanoic acid
-
0.0427
3-[(1-amino-2-carboxyethyl)(hydroxy)phosphoryl]-2-methylpropanoic acid
-
0.1831
3-[[amino(carboxy)methyl](hydroxy)phosphoryl]-2-methylpropanoic acid
-
0.105
5-amino-5-phosphonopentanoic acid
-
0.0000009
Ala-PSI[PO2-CH2]-Leu-Ala
-
pH 7.4, 25C
0.0000059 - 0.0001927
amastatin
0.0271
amino(phosphono)acetic acid
-
0.395
amino[hydroxy(methyl)phosphoryl]acetic acid
-
0.00026 - 0.001297
angiotensin IV
0.0141 - 0.0289
captopril
0.0019 - 0.016
D-glutamate phosphonic acid
0.00025
EC33
-
-
0.000194
Glu-PSI[PO2-CH2]-Leu
-
pH 7.4, 25C
0.0000008
Glu-PSI[PO2-CH2]-Leu-Ala
-
pH 7.4, 25C
0.00003
Glu-PSI[PO2-CH2]-Leu-Arg
-
pH 7.4, 25C
0.0000057
Glu-PSI[PO2-CH2]-Leu-Asp
-
pH 7.4, 25C
0.000037
Glu-PSI[PO2-CH2]-Leu-Leu
-
pH 7.4, 25C
0.000038 - 0.056
glutamate phosphonic acid
0.00038 - 0.00206
glutamate thiol
0.00012 - 0.01
glutamate-thiol
0.000462 - 0.00202
glutamine-thiol
0.000045 - 0.021
L-glutamate phosphonic acid
0.0001 - 0.00394
lysine thiol
0.00046 - 0.0199
lysine-thiol
0.00019 - 0.0084
methionine thiol
0.00007
N-(2-aminoethyl)-4-methyl-5-sulfanyl-pentanamide
-
wild type enzyme, in 50 mM Tris/HCl buffer (pH 7.4), 4 mM Ca2+, at 37C
0.00025
[(S)-3-amino-4-mercaptobutyl sulfonic acid]
-
-
0.000292
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
-
pH 7.3, 37C
0.0000833
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
-
pH 7.3, 37C
0.000053 - 0.00047
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
0.000283
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
-
pH 7.3, 37C
0.0042
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
-
pH 7.3, 37C
0.0012
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
-
pH 7.3, 37C
0.000027
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
-
pH 7.3, 37C
0.0000733
[[(2R,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
-
pH 7.3, 37C
0.000267
[[(2R,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
0.00091
[[(2R,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
0.00005
[[(2R,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
0.000016
[[(2R,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
0.000051
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentane]-Ile-Asp-OH
-
pH 7.3, 37C
0.00008
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
0.00015
[[(2RS,3RS)-3-amino2-sulfhydryl-5-sulfamoyl]pentanoyl]-Tyr-Asp-OH
-
pH 7.3, 37C
0.0000147
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-D-Ile-Asp-OH
-
pH 7.3, 37C
0.0000036
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-3-sulfoalanine-OH
-
pH 7.3, 37C
0.0000032 - 0.000015
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
0.00000536
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-D-Asp-OH
-
pH 7.3, 37C
0.000145
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-NH2
-
pH 7.3, 37C
0.00004
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-OH
-
pH 7.3, 37C
0.0000043
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-Asp-OH
-
pH 7.3, 37C
0.0000093
[[(2S,3R)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Tyr-homosulfoalanine-OH
-
pH 7.3, 37C
0.00000356
[[(2S,3R)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
0.000012
[[(2S,3R)-3-amino-5-phosphonate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
0.00002
[[(2S,3S)-3-amino-2-sulfhydryl-5-sulfonate]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
0.000013
[[(2S,3S)-3-amino-5-carboxylate-2-sulfhydryl]pentanoyl]-Ile-Asp-OH
-
pH 7.3, 37C
additional information
additional information
-
inhibition constant of 3-amino-4-thio-butyl sulfonic acid for aminopeptidase A: 0.29 microM/L
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.06
1,10-phenanthroline
Homo sapiens
-
IC50 is 0.06 mM
0.000157
amastatin
Homo sapiens
-
IC50 is 157 nM
0.065
angiotensin II
Homo sapiens
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.065 mM in presence of Ca2+
0.01
angiotensin IV
Homo sapiens
-
IC50 is 0.010 mM
0.024
cholecystokinin-8
Homo sapiens
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.024 mM in presence of Ca2+
0.049
chromogranin A
Homo sapiens
-
competitive against Glu-4-methylcoumaryl-7-amide, IC50 is 0.049 mM in presence of Ca2+
-
0.06
neurokinin B
Homo sapiens
-
competitive against Glu-4-methylcoumaryl-7-amide,IC50 is 0.060 mM in presence of Ca2+
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000003
-
substrate Glu-4-methyl-coumaryl-7-amide
0.000004
-
soluble glutamyl aminopeptidase activity in the atrium of euthyroid adult male rats; soluble glutamyl aminopeptidase activity in the atrium of hyperthyroid adult male rats; soluble glutamyl aminopeptidase activity in the atrium of hypothyroid adult male rats
0.0000071
0.0000138
-
substrate Glu-4-methyl-coumaryl-7-amide
0.000014
0.000028
-
plasma enzyme, 37C
0.000045
-
membrane-bound glutamyl aminopeptidase activity in the atrium of hypothyroid adult male rats
0.000046
-
plasma enzyme, 37C
0.00005
-
membrane-bound glutamyl aminopeptidase activity in the atrium of euthyroid adult male rats
0.00006
-
plasma enzyme, 20C
0.000065
-
membrane-bound glutamyl aminopeptidase activity in the atrium of hyperthyroid adult male rats
0.000069
-
plasma enzyme, 20C
0.00007
-
plasma enzyme, 37C
0.000686
-
DOCA-salt rat, 30 mg/kg RB150, 3.5 h after po
0.00071
-
DOCA-salt rat, 50 mg/kg RB150, 3.5 h after po
0.000726
-
DOCA-salt rat, 15 mg/kg RB150, 3.5 h after po
0.000728
-
normotensive WKY rat, 15 mg/kg RB150, 3.5 h after po
0.000752
-
normotensive WKY rat, 3.5 h after administration of saline po
0.00077
-
normotensive Sham rat, 3.5 h after administration of saline po
0.001083
-
DOCA-salt rat, 7.5 mg/kg RB150, 3.5 h after po
0.001525
-
DOCA-salt rat, 3.5 h after administration of saline po
0.001557
-
DOCA-salt rat, 15 mg/kg RB150, 24 h after po
0.06
-
aminopeptidase A activity, cortex, aminopeptidase A-deficient mice; aminopeptidase A activity, striatum, aminopeptidase A-deficient mice
0.09
-
aminopeptidase A activity, tegmentum/colliculi, aminopeptidase A-deficient mice
0.25
-
mutant enzyme T348Y, in the absence of Ca2+
0.4
-
aminopeptidase A activity, striatum, wild-type mice
0.45
-
aminopeptidase A activity, cortex, wild-type mice
0.629
-
-
0.8
-
mutant enzyme T348D, in the absence of Ca2+
1.4
-
aminopeptidase A activity, tegmentum/colliculi, wild-type mice
1.6
-
mutant enzyme T348D, in the presence of 4 mM Ca2+
1.98
-
wild type enzyme, in the absence of Ca2+
3.05
-
mutant enzyme T348S, in the absence of Ca2+
7
-
aminopeptidase A activity depending on alcohol, lung, wild-type mice, with access to a 10% ethanol solution
7.4
-
mutant enzyme T348Y, in the presence of 4 mM Ca2+
9.88
-
1 mM Glu-p-nitranilide in 0.05 M Tris/HCl, pH 7.5
11
-
aminopeptidase A activity depending on alcohol, lung, wild-type mice, without access to a 10% ethanol solution
12
-
ATA I
14.67
-
wild type enzyme, in the presence of 4 mM Ca2+
19.76
-
-
21
-
aminopeptidase A activity depending on alcohol, kidney, wild-type mice, without access to a 10% ethanol solution
22.8
-
-
26
-
aminopeptidase A activity depending on alcohol, kidney, wild-type mice, with access to a 10% ethanol solution
31.54
-
mutant enzyme T348S, in the presence of 4 mM Ca2+
36.1
-
-
40
-
ATA II
54.9
-
-
66.3
-
substrate Glu-Glu, pH 7.2, 37C
132
-
substrate Asp-4-nitroanilide, pH 7.2, 37C
319
-
substrate Glu-4-nitroanilide, pH 7.2, 37C
607
Glu-7-amido-4-methylcoumarin
662.5
Asp-7-amido-4-methylcoumarin
additional information