Information on EC 3.4.11.6 - aminopeptidase B

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.4.11.6
-
RECOMMENDED NAME
GeneOntology No.
aminopeptidase B
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of N-terminal Arg and Lys from oligopeptides when P1' is not Pro. Also acts on arylamides of Arg and Lys
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
CAS REGISTRY NUMBER
COMMENTARY hide
9073-92-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
ywad gene
SwissProt
Manually annotated by BRENDA team
strain ATCC 51502
-
-
Manually annotated by BRENDA team
marine psychrophile, strain 34H
-
-
Manually annotated by BRENDA team
Corycaeus anglicus
-
-
-
Manually annotated by BRENDA team
strain CECT 12487
-
-
Manually annotated by BRENDA team
strain CECT 12487
-
-
Manually annotated by BRENDA team
K 12
-
-
Manually annotated by BRENDA team
rainbow trout
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
var. Alaska
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strains ATCC 27853 and ATCC 33348
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
male gilthead sea bream
-
-
Manually annotated by BRENDA team
FSS2
SwissProt
Manually annotated by BRENDA team
FSS2
SwissProt
Manually annotated by BRENDA team
903
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
haploid fungus, strain FB1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
when murine ACE2 is given to mice for 4 weeks, a marked increase in serum ACE2 activity is detected. ACE2 leads to the dissipation of Ang II with formation of Ang (1-7)
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-2-naphthylamide + H2O
Ala + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
show the reaction diagram
-
4.6% of the activity with Leu-4-nitroanilide
-
?
Ala-Ala 4-nitroanilide + H2O
Ala-Ala + 4-nitroaniline
show the reaction diagram
alpha-Glu-4-nitroanilide + H2O
Glu + 4-nitroaniline
show the reaction diagram
-
564% of the activity with Leu-4-nitroanilide
-
?
angiotensin III + H2O
angiotensin IV + ?
show the reaction diagram
-
-
-
-
?
Arg 4-methylcoumarin 7-amide + H2O
Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Arg-4-methylcoumarin 7-amide + H2O
Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Arg-4-methylcoumaryl-7-amide + H2O
Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
show the reaction diagram
Arg-7-amido-4-methylcoumarin + H2O
Arg + 7-amino-4-methylcoumarin
show the reaction diagram
Arg-Ala + H2O
Arg + Ala
show the reaction diagram
-
82% of the activity with Arg-Arg
-
?
Arg-alpha-atrial natriuretic factor1-20 + H2O
Arg + alpha-atrial natriuretic factor1-20
show the reaction diagram
-
-
-
-
?
Arg-Arg + H2O
Arg + Arg
show the reaction diagram
-
-
-
?
Arg-Asp + H2O
Arg + Asp
show the reaction diagram
-
25% of the activity with Arg-Arg
-
?
Arg-beta-atrial natriuretic factor1-20 + H2O
Arg + beta-atrial natriuretic factor1-20
show the reaction diagram
-
-
-
-
?
Arg-beta-naphthylamide + H2O
L-arginine + 2-naphthylamine
show the reaction diagram
-
substrate used in the activity assay
-
-
?
Arg-Gly + H2O
Arg + Gly
show the reaction diagram
-
-
?
Arg-Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro + H2O
Arg + Gly-Asp-Ser-Pro-Ala-Ser-Ser-Lys-Pro
show the reaction diagram
i.e. fibronectin-binding inhibitor
-
?
Arg-Gly-Glu-Ser + H2O
Arg + Gly-Glu-Ser
show the reaction diagram
i.e. platelet aggregation inhibitor
-
?
Arg-Gly-Pro-Phe-Pro-Ile + H2O
Arg + Gly-Pro-Phe-Pro-Ile
show the reaction diagram
i.e. sexual agglutination peptide
-
?
Arg-Gly-Tyr-Ala-Leu-Gly + H2O
Arg + Gly-Tyr-Ala-Leu-Gly
show the reaction diagram
-
-
-
-
?
Arg-Ile + H2O
Arg + Ile
show the reaction diagram
-
32% of the activity with Arg-Arg
-
?
Arg-Leu + H2O
Arg + Leu
show the reaction diagram
-
25% of the activity with Arg-Arg
-
?
Arg-Leu-enkephalin + H2O
Arg + Leu-enkephalin
show the reaction diagram
-
-
-
-
?
Arg-Lys + H2O
Arg + Lys
show the reaction diagram
-
88% of the activity with Arg-Arg
-
?
Arg-Lys-Asp-Val-Tyr-OH + H2O
?
show the reaction diagram
-
-
-
-
?
Arg-Lys-somatostatin-14 + H2O
Arg + Lys + somatostatin-14
show the reaction diagram
-
sequential removal of basic N-terminal residues
-
-
?
Arg-Lys-somatostatin-14 + H2O
Arg-Lys + somatostatin-14
show the reaction diagram
-
-
-
-
?
Arg-Met-enkephalin + H2O
?
show the reaction diagram
-
-
-
-
?
Arg-Met-enkephalin + H2O
Arg + Met-enkephalin
show the reaction diagram
-
-
-
-
?
Arg-neurokinin A + H2O
Arg + neurokinin A
show the reaction diagram
-
-
-
-
?
Arg-peptides + H2O
?
show the reaction diagram
Arg-Phe + H2O
Arg + Phe
show the reaction diagram
Arg-Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-Lys-Asn-Val + H2O
Arg + Phe-Ala-Arg-Lys-Gly-Ala-Leu-Arg-Gln-Lys-Asn-Val
show the reaction diagram
i.e. protein kinase C substrate
-
?
Arg-Pro + H2O
Arg + Pro
show the reaction diagram
Arg-Pro-Lys-Pro-Gln-Gly-Leu-Met + H2O
Arg + Pro-Lys-Pro-Gln-Gly-Leu-Met
show the reaction diagram
i.e. substance P
-
?
Arg-Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg + H2O
Arg + Pro-Pro-Gly-Phe-Ser-Pro-Phe-Arg
show the reaction diagram
Arg-Tyr-Leu-Pro-Thr + H2O
Arg + Tyr-Leu-Pro-Thr
show the reaction diagram
-
proctolin
-
-
?
Arg-Val-Tyr-Ile-His-Pro-Ile + H2O
Arg + Val-Tyr-Ile-His-Pro-Ile
show the reaction diagram
i.e. angiotensin III
-
?
Arg-Val-Tyr-Ile-His-Pro-Phe + H2O
Arg + Val-Tyr-Ile-His-Pro-Phe
show the reaction diagram
-
angiotensin III
-
-
?
azocasein + H2O
?
show the reaction diagram
-
-
-
-
?
azocasein + H2O
protein fragments of azocasein of MW 71 kDa, 83 kDa, and 22 kDa
show the reaction diagram
-
-
-
-
?
benzoyl-Arg-p-nitroanilide + H2O
benzoyl-Arg + p-nitroaniline
show the reaction diagram
-
-
-
-
?
Cys-Gly + H2O
Cys + Gly
show the reaction diagram
-
4210% of the activity with Leu-4-nitroanilide
-
?
elastin Congo red + H2O
?
show the reaction diagram
-
-
-
-
?
glucagon + H2O
miniglucagon + ?
show the reaction diagram
-
-
-
-
?
Gly-2-naphthylamide + H2O
Gly + 2-naphthylamine
show the reaction diagram
Gly-4-nitroanilide + H2O
Gly + 4-nitroaniline
show the reaction diagram
-
1.3% of the activity with Leu-4-nitroanilide
-
?
Gly-L-Pro-2-naphthylamide + H2O
Gly-L-Pro + 2-naphthylamine
show the reaction diagram
-
1% activity compared to L-Lys-2-naphthylamide
-
-
?
Gly-Phe 4-nitroanilide + H2O
Gly-Phe + 4-nitroaniline
show the reaction diagram
kallidin + H2O
?
show the reaction diagram
-
-
-
-
?
kallidin-10 + H2O
Lys + bradykinin
show the reaction diagram
-
-
-
-
?
L-Ala-2-naphthylamide + H2O
L-Ala + 2-naphthylamine
show the reaction diagram
L-Ala-4-nitroanilide + H2O
L-Ala + 4-nitroaniline
show the reaction diagram
L-Ala-7-amido-4-methylcoumarin + H2O
L-Ala + 7-amino-4-methylcoumarin
show the reaction diagram
L-Ala-Ala-Pro-Leu-4-nitroanilide + H2O
L-Ala-Ala-Pro-Leu + 4-nitroaniline
show the reaction diagram
-
-
-
-
?
L-Arg 7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
-
fluorogenic substrate, best substrate
-
-
?
L-Arg-(Met)enkephalin + H2O
L-Arg + (Met)enkephalin
show the reaction diagram
L-Arg-2-naphthylamide + H2O
L-Arg + 2-naphthylamine
show the reaction diagram
L-Arg-4-nitroanilide + H2O
L-Arg + 4-nitroaniline
show the reaction diagram
L-Arg-7-amido-4-methylcoumarin + H2O
L-Arg + 7-amino-4-methylcoumarin
show the reaction diagram
L-Arg-p-nitroanilide + H2O
L-Arg + p-nitroaniline
show the reaction diagram
L-Arg-peptide + H2O
L-Arg + peptide
show the reaction diagram
-
specific for N-terminal Arg or Lys residues, di-, tri-, and polypeptides
-
-
?
L-arginine-2-naphthylamide + H2O
2-naphthylamine + L-Arg
show the reaction diagram
-
assay at 37C
-
-
?
L-Asn-7-amido-4-methylcoumarin + H2O
L-Asn + 7-amino-4-methylcoumarin
show the reaction diagram
53% activity compared to L-Arg-7-amido-4-methylcoumarin
-
-
?
L-Asp-7-amido-4-methylcoumarin + H2O
L-Asp + 7-amino-4-methylcoumarin
show the reaction diagram
15% activity compared to L-Arg-7-amido-4-methylcoumarin
-
-
?
L-beta-Asn-2-naphthylamide + H2O
L-beta-Asn + 2-naphthylamine
show the reaction diagram
-
9% activity compared to L-Lys-2-naphthylamide
-
-
?
L-Cys-2-naphthylamide + H2O
L-Cys + 2-naphthylamine
show the reaction diagram
-
1% activity compared to L-Lys-2-naphthylamide
-
-
?
L-Cys-7-amido-4-methylcoumarin + H2O
L-Cys + 7-amino-4-methylcoumarin
show the reaction diagram
-
5% activity compared to L-Arg-7-amido-4-methylcoumarin
-
-
?
L-Gly-L-Arg-2-naphthylamide + H2O
L-Gly-L-Arg + 2-naphthylamine
show the reaction diagram
-
250% activity compared to L-Lys-2-naphthylamide
-
-
?
L-His-2-naphthylamide + H2O
L-His + 2-naphthylamine
show the reaction diagram
-
15% activity compared to L-Lys-2-naphthylamide
-
-
?
L-Leu 7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
show the reaction diagram
-
fluorogenic substrate
-
-
?
L-Leu-2-naphthylamide + H2O
L-Leu + 2-naphthylamine
show the reaction diagram
L-Leu-4-nitroanilide + H2O
L-Leu + 4-nitroaniline
show the reaction diagram
L-Leu-7-amido-4-methylcoumarin + H2O
L-Leu + 7-amino-4-methylcoumarin
show the reaction diagram
L-Lys-(Met)enkephalin + H2O
L-Lys + (Met)enkephalin
show the reaction diagram
L-Lys-2-naphthylamide + H2O
L-Lys + 2-naphthylamine
show the reaction diagram
L-Lys-4-nitroanilide + H2O
L-Lys + 4-nitroaniline
show the reaction diagram
L-Lys-7-amido-4-methylcoumarin + H2O
L-Lys + 7-amino-4-methylcoumarin
show the reaction diagram
L-Lys-L-Ala-2-naphthylamide + H2O
L-Lys-L-Ala + 2-naphthylamine
show the reaction diagram
-
7% activity compared to L-Lys-2-naphthylamide
-
-
?
L-Lys-p-nitroanilide + H2O
L-Lys + p-nitroaniline
show the reaction diagram
-
-
-
-
?
L-Lys-peptide + H2O
L-Lys + peptide
show the reaction diagram
-
specific for N-terminal Arg or Lys residues, di-, tri-, and polypeptide
-
-
?
L-lysine-2-naphthylamide + H2O
2-naphthylamine + L-Lys
show the reaction diagram
-
assay at 37C
-
-
?
L-Met-2-naphthylamide + H2O
L-Met + 2-naphthylamine
show the reaction diagram
L-Met-7-amido-4-methylcoumarin + H2O
L-Met + 7-amino-4-methylcoumarin
show the reaction diagram
L-Phe-2-naphthylamide + H2O
L-Phe + 2-naphthylamine
show the reaction diagram
-
0.171% activity compared to L-Arg-2-naphthylamide
-
-
?
L-Phe-7-amido-4-methylcoumarin + H2O
L-Phe + 7-amino-4-methylcoumarin
show the reaction diagram
L-Pro-7-amido-4-methylcoumarin + H2O
L-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
7% activity compared to L-Arg-7-amido-4-methylcoumarin
-
-
?
L-Ser-7-amido-4-methylcoumarin + H2O
L-Ser + 7-amino-4-methylcoumarin
show the reaction diagram
15% activity compared to L-Arg-7-amido-4-methylcoumarin
-
-
?
L-Tyr 7-amido-4-methylcoumarin + H2O
L-Tyr + 7-amino-4-methylcoumarin
show the reaction diagram
-
fluorogenic substrate
-
-
?
L-Tyr-2-naphthylamide + H2O
L-Tyr + 2-naphthylamine
show the reaction diagram
L-Val-2-naphthylamide + H2O
L-Val + 2-naphthylamine
show the reaction diagram
-
0.034% activity compared to L-Arg-2-naphthylamide
-
-
?
L-Val-4-nitroanilide + H2O
L-Val + 4-nitroaniline
show the reaction diagram
low activity
-
-
?
Leu-2-naphthylamide + H2O
Leu + 2-naphthylamine
show the reaction diagram
-
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
show the reaction diagram
Leu-Gly + H2O
Leu + Gly
show the reaction diagram
-
1200% of the activity with Leu-4-nitroanilide
-
?
leukotriene A4 + H2O
L-Arg + leukotriene B4
show the reaction diagram
-
weak in vitro activity
-
-
?
leukotriene A4 + H2O
leukotriene B4 + ?
show the reaction diagram
-
has a residual catalytic ability to hydrolyze leukotriene A4
-
-
?
Lys 4-methylcoumarin 7-amide + H2O
Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
-
-
-
?
Lys-2-naphthylamide + H2O
Lys + 2-naphthylamine
show the reaction diagram
Lys-4-methylcoumarin 7-amide + H2O
Lys + 7-amino-4-methylcoumarin
show the reaction diagram
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
show the reaction diagram
Lys-7-amido-4-methylcoumarin + H2O
Lys + 7-amino-4-methylcoumarin
show the reaction diagram
-
4.3% of the activity with Arg-7-amido-4-methylcoumarin
-
?
Lys-Ala + H2O
Lys + Ala
show the reaction diagram
-
88% of the activity with Arg-Arg
-
?
Lys-Leu-2-naphthylamide + H2O
Lys + Leu-2-naphthylamide
show the reaction diagram
-
-
-
-
?
Lys-Lys + H2O
Lys + Lys
show the reaction diagram
-
30% of the activity with Arg-Arg
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
show the reaction diagram
-
40.6% of the activity with Leu-4-nitroanilide
-
?
Met-enkephalin + H2O
?
show the reaction diagram
-
-
-
-
?
N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O
N-p-tosyl-Gly-Pro-Lys + p-nitroaniline
show the reaction diagram
-
-
-
-
?
N-succinyl-Gly-Pro-Leu-Gly-Pro 7-amido-4-methylcoumarin + H2O
N-succinyl-Gly-Pro-Leu-Gly-Pro + 7-amino-4-methylcoumarin
show the reaction diagram
-
fluorogenic substrate
-
-
?
Poly-L-lysine + H2O
?
show the reaction diagram
-
-
-
-
?
thymopentin + H2O
?
show the reaction diagram
-
-
-
-
?
Val-4-nitroanilide + H2O
Val + 4-nitroaniline
show the reaction diagram
-
1.2% of the activity with Leu-4-nitroanilide
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Arg-peptides + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Br-
-
activation
Cu2+
-
; the metal-substituted derivative Cu(II)-Ap-B contains almost 1 mol of copper(II) ions per molecule
Fe2+
-
inhibitor
Fe3+
-
29% activation at 0.1 mM
Hg2+
-
inhibitor
Li+
-
0.4 M activates the activity by 45%
Mg2+
-
22% activation at 10
NH4Cl
-
enhances activity, maximal activation at 100 mM
Sn2+
-
slight enzyme activation
Zinc
essential zinc-binding site HEXXH at amino acid residues 370-374 with a second glutamic acid separated by 18 amino acids
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
1-methyl cyclohexan bestatin
-
i.e. BE15, strong inhibition
2,2'-dipyridyl
-
-
2,4-Dinitrofluorobenzene
-
-
2,6-pyridinedicarboxylate
-
-
2-Chloroethylphosphonic acid
-
-
2-mercaptoethanol
3,4-dichloroisocoumarin
4-chloromercuribenzoate
-
-
5,5 dithiobis(2-nitrobenzoic acid)
-
1 mM, 74.89% enzyme inhibition
5,5'-dithiobis(2-nitrobenzoate)
8-hydroxyquinoline
-
-
Acetate buffer
-
-
actinonin
Ag+
-
complete inhibition at 0.1 mM
amastatin
amino acids
antipain
-
-
Aprotinin
-
34% residual activity at 0.25 mg/ml
apstatin
0.1 mM, 50% inhibition
arginine derivatives
-
with carboxyl or alpha-amino group blocked
arphamemine B
-
-
arphamenine
-
aminopeptidase B-specific inhibitor
-
Arphamenine A
Arphamenine B
bestatin
beta-mercaptoethanol
bipyridine
-
-
Borax-pyruvic acid buffer
-
-
-
cementoin
-
-
-
citric acid-sodium citrate buffer
-
-
-
Cyclopeptide OF49-II
-
from Penicillium regulosum, total synthesis
-
cysteine
D,L-Homolysine
-
-
D-Arg
-
-
D-penicillamine
-
-
DFP
5 mM, 25% inhibition. 10 mM, 44% inhibition
dithiothreitol
DX600
-
only blocks human ACE2 activity but not mouse
E-64
-
99% residual activity at 0.1 mM
ethanol
-
-
ethyliodoacetate
-
-
Glu-pyrrolidide
0.1 mM, 98% inhibition
glutathione
-
high concentration
Gly-Gly
5 mM, 8% inhibition
HgCl2
-
complete inhibition at 1 mM
iodoacetamide
iodoacetate
-
0.1 mM, complete inactivation
Iodobenzoate
-
-
-
kallidin
-
human peptide hormone, 10 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 73.7% (wild-type enzyme)
L-1-tosylamido-2-phenylethylchloromethylketone
-
-
L-Arg
5 mM, 70% inhibition
L-Arg-2-naphthylamide
-
competitive inhibition of hydrolysis of L-Ala-2-naphthylamide, no inhibition vice versa
L-Lys
5 mM, 28% inhibition
L-Lysinethiol
-
-
L-Penicillamine
-
-
Leucine hydroxamate
-
-
leucinthiol
-
-
leuhistin
-
40% inhibition at 0.0003 mM, independent on TGF-beta1 activation
Leupeptin
methyl bestatin
-
-
Mg2+
-
slight inhibition at 1 mM
MgCl2
-
0.1 mM MgCl2, 22% inhibition
MLN-4760
N-alpha-p-Tosyl-L-lysine
-
-
N-alpha-p-tosyl-L-lysine-chloromethyl ketone
-
specific and irreversible inhibitor
N-ethylmaleimide
N-[(2S,3R)-3-amino-2-hydroxy-5-methylhexanoyl]-L-valyl-L-valyl-L-aspartic acid
-
-
N-[1-(R,S)-carboxy-3-phenyl propyl] Ala-Ala-Phe-p-aminobenzoate
-
-
Na+
-
NaCl (0.1-100 mM) has an opposite effect on the EGTA-treated KAP apo-enzyme, it inhibits 13% at 0.1 mM and 100% at 100 mM
NEM
-
13% inhibition at 1 mM
nitrobestatin
-
92.3% inhibition at 0.133 mM
o-phenanthroline
-
the native enzyme is inhibited by 76% at 0.5 mM, the recombinant enzyme is inhibited by 80% at 0.5 mM
p-aminophenylmercuric acetate
-
1 mM, 41.27% enzyme inhibition
p-chloromercuribenzene sulfonic acid
-
-
p-chloromercuribenzoate
p-hydroxymercuriphenyl sulfonic acid
-
1 mM, 72.22% enzyme inhibition
p-Hydroxymercuriphenylsulfonic acid
-
-
PCMB
2 mM, complete inactivation
pefabloc
Pepstatin
Phe
-
noncompetitive
Phenylmethanesulfonylfluoride
phosphate
-
-
Porphyrindine
-
-
pre-elafin
-
-
-
puromycin
Substance P
-
human peptide hormone, 100 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 63.8% (wild-type enzyme); human peptide hormone, 10 microM, Arg 4-methylcoumarin 7-amide hydrolysis: 78% (wild-type enzyme)
tert-butyl bestatin
-
i.e. BE17, the BE15 derivative has a dual inhibitory effect of invasion and motility on tumor and endothelial cells
thioglycolic acid
-
-
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
acetate
-
activation
bacitracin
-
activation
cysteine
-
slight activation
diphosphate
-
activation in presence of low Cl- concentrations
dithiothreitol
E64
-
slight activation at 0.01-0.05 mM
L-Cys
5 mM, activation to 148% of control
NaF
-
0.4 M activates the activity by 20%
phosphate
-
activation
sulfhydryl compounds
-
activation, enzyme I
TGF-beta1
-
activates aminopeptidase activity
-
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0533 - 0.0774
Arg 4-methylcoumarin 7-amide
0.00318
Arg-4-methylcoumaryl-7-amide
pH 7.5, 37C
0.051
Arg-4-nitroanilide
pH 7.8, 37C
0.0159
Arg-7-amido-4-methylcoumarin
-
pH 5.0, 37C
0.014
Arg-alpha-atrial natriuretic factor1-20
-
-
-
0.183 - 0.267
Arg-beta-naphthylamide
0.111
Arg-Leu-enkephalin
-
-
0.005
Arg-Lys-somatostatin-14
-
-
0.021 - 0.125
Arg-Met-enkephalin
0.058
Arg-neurokinin A
-
-
0.07 - 0.27
arginyl-4-methylcoumarin 7-amide
7.8
Cys-Gly
-
pH 7.5, 37C
0.0157 - 0.0651
kallidin
10
L-Ala-4-nitroanilide
pH 8.0, 30C
0.043 - 0.072
L-Ala-Ala-Pro-Leu-4-nitroanilide
0.00003 - 0.162
L-Arg-2-naphthylamide
0.6
L-Arg-4-nitroanilide
pH 8.0, 30C
0.048 - 0.091
L-Arg-7-amido-4-methylcoumarin
0.0026 - 0.3
L-Arg-beta-naphthylamide
3.6
L-Leu-4-nitroanilide
pH 8.0, 30C
0.00014 - 0.333
L-Lys-2-naphthylamide
0.0544 - 0.85
L-Lys-4-nitroanilide
0.12 - 0.36
L-Lys-beta-naphthylamide
1.2
L-Lys-p-nitroanilide
-
-
0.000124 - 0.000197
L-Tyr-2-naphthylamide
19
L-Val-4-nitroanilide
pH 8.0, 30C
4.9
Leu-4-nitroanilide
-
pH 7.5, 37C
13.5
Leu-Gly
-
pH 7.5, 37C
0.381 - 0.568
Lys 4-methylcoumarin 7-amide
0.026
Lys-7-amido-4-methylcoumarin
-
pH 5.0, 37C
0.28 - 0.4
lysyl-4-methylcoumarin 7-amide
0.0251 - 0.36
Met-enkephalin
0.1 - 0.2
N-naphthalen-2-yl-L-argininamide
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.06 - 9.27
Arg 4-methylcoumarin 7-amide
2.3
Arg-4-methylcoumaryl-7-amide
Homo sapiens
Q6P179
pH 7.5, 37C
53.1 - 69.5
Arg-beta-naphthylamide
1.07 - 3.63
Arg-Met-enkephalin
10700
Cys-Gly
Escherichia coli
-
pH 7.5, 37C
1.99 - 2.35
kallidin
0.37
L-Ala-4-nitroanilide
Bacillus subtilis
P25152
pH 8.0, 30C
0.043 - 0.36
L-Ala-Ala-Pro-Leu-4-nitroanilide
0.49 - 69.47
L-Arg-2-naphthylamide
34
L-Arg-4-nitroanilide
Bacillus subtilis
P25152
pH 8.0, 30C
55
L-Leu-4-nitroanilide
Bacillus subtilis
P25152
pH 8.0, 30C
0.037 - 79.3
L-Lys-2-naphthylamide
10
L-Lys-4-nitroanilide
Bacillus subtilis
P25152
pH 8.0, 30C
1 - 2.1
L-Tyr-2-naphthylamide
0.43
L-Val-4-nitroanilide
Bacillus subtilis
P25152
pH 8.0, 30C
307
Leu-4-nitroanilide
Escherichia coli
-
pH 7.5, 37C
6630
Leu-Gly
Escherichia coli
-
pH 7.5, 37C
1.95 - 14.9
Lys 4-methylcoumarin 7-amide
0.47 - 0.48
Met-enkephalin
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
52400 - 175000
Arg 4-methylcoumarin 7-amide
10.4 - 173
Arg-Met-enkephalin
30.1 - 156
kallidin
48 - 157
L-Arg-2-naphthylamide
3440 - 39200
Lys 4-methylcoumarin 7-amide
1 - 19
Met-enkephalin
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
1,10-phenanthroline
-
-
0.02
actinonin
-
at 37C in Bicine buffer complemented with 0.2 M NaCl
0.0125
arphamemine B
-
at 37C in Bicine buffer complemented with 0.2 M NaCl
0.0000076 - 0.0000085
Arphamenine A
0.0000085 - 0.0000103
Arphamenine B
0.00021
bestatin
-
at 37C in Bicine buffer complemented with 0.2 M NaCl
0.024
Leucine hydroxamate
-
at 37C in Bicine buffer complemented with 0.2 M NaCl
0.00054
leucinthiol
-
at 37C in Bicine buffer complemented with 0.2 M NaCl
0.006
Zn2+
-
in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, at 37C
additional information
additional information
-
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00006 - 0.000077
1,10-phenanthroline
0.00000316 - 0.00000373
Arphamenine A
0.00000072 - 0.00000155
Arphamenine B
0.00000349 - 0.000289
bestatin
0.00000391 - 0.00000592
ZnCl2
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00011
-
plasma enzyme, 37C
0.00012
-
plasma enzyme, 20C
0.0003
-
plasma enzyme, 20C
0.00031
-
plasma enzyme, 20C
0.00082
-
plasma enzyme, 37C
0.0011
-
plasma enzyme, 37C
0.0016
-
normal healthy rats and rats treated/deprived with salt and water, soluble fraction of adrenal gland
0.0018
-
normal healthy rats and rats treated/deprived with salt and water, soluble fraction of lung
0.002
-
normal healthy rats and rats treated/deprived with salt and water, soluble fraction of heart
0.0024
-
normal healthy rats and rats treated/deprived with salt and water, soluble fraction of kidney cortex
0.0042
-
normal healthy rats and rats treated/deprived with salt and water, soluble fraction of kidney medulla
0.06708
-
after purification
0.1
-
Cu(II)-Ap-B; metal-substituted enzyme form Cu(II)-Ap-B, using L-Arg-2-naphthylamide as a substrate, at pH 7.4 and 25C
0.63
-
purified enzyme
2.16
-
-
12.5
-
Ap-B; recombinant Ap-B, using L-Arg-2-naphthylamide as a substrate, at pH 7.4 and 25C
55
-
purified enzyme, substrate L-Leu 7-amido-4-methylcoumarin
64
purified recombinant enzyme
263.7
-
purified enzyme, substrate L-Arg 7-amido-4-methylcoumarin
297.9
-
purified enzyme
6974
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 6.5
7.8
-
immobilized enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5 - 5.5
-
pH 4.5: about 80% of maximal activity, pH 5.5: about 25% of maximal activity, activity is negligible at pH values beyond pH 4.0 and pH 6.0
5.5 - 8.25
-
-
6 - 9
-
pH profile
7 - 9
-
narrow pH-optimum
7.5 - 9.5
-
-
additional information
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
activity assay
28 - 32
-
-
45
-
free enzyme
50
-
immobilized enzyme
additional information
-
the enzyme is cold-active
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 45
-
15C: about 30% of maximal activity, 25C: about 75% of maximal activity, 45C: about 70% of maximal activity
20 - 37
35 - 80
-
-
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7
-
chromatofocusing
5.1
-
isoelectric focusing
5.3
calculated from amino acid sequence
5.6 - 5.9
isoelectric focusing
5.83
-
theoretical
7.5
-
band I, isoelectric fosusing
8.8
-
band II, isoelectric fosusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
neuroblastoma cell line
Manually annotated by BRENDA team
-
colorectal adenocarcinoma cell line
Manually annotated by BRENDA team
-
B-cell line
Manually annotated by BRENDA team
-
high enzyme activity
Manually annotated by BRENDA team
-
cardiac, ventricular
Manually annotated by BRENDA team
-
pituitary tumor-derived cell line
Manually annotated by BRENDA team
-
neutrophilic promyelocyte cell line
Manually annotated by BRENDA team
-
T-cell line
Manually annotated by BRENDA team
-
lung cell line
Manually annotated by BRENDA team
-
adrenal pheochromocytoma cell line
Manually annotated by BRENDA team
-
enzyme probably derived from blood
Manually annotated by BRENDA team
-
somatic, testis
Manually annotated by BRENDA team
-
primary pituitary gland cell line
Manually annotated by BRENDA team
-
lymphoblast-like cell line
Manually annotated by BRENDA team
enzyme expression from embryo to adult rat, neuronal cell, developmental distribution, overview
Manually annotated by BRENDA team
-
high enzyme activity
Manually annotated by BRENDA team
-
high enzyme activity
Manually annotated by BRENDA team
-
high enzyme activity
Manually annotated by BRENDA team
additional information