Information on EC 3.4.11.23 - PepB aminopeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.11.23
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RECOMMENDED NAME
GeneOntology No.
PepB aminopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
release of an N-terminal amino acid, Xaa, from a peptide or arylamide. Xaa is preferably Glu or Asp but may be other amino acids, including Leu, Met, His, Cys and Gln
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glutathione metabolism
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Metabolic pathways
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CAS REGISTRY NUMBER
COMMENTARY hide
9056-26-2
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928346-44-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-Leu + H2O
Ala + Leu
show the reaction diagram
Asn-Leu + H2O
Asn + Leu
show the reaction diagram
Asp-Ile + H2O
Asp + Ile
show the reaction diagram
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best substrate, 120% activity compared to substrate Asp-Leu
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?
Asp-Leu + H2O
Asp + Leu
show the reaction diagram
Asp-Leu-Gly + H2O
Asp + Leu-Gly
show the reaction diagram
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higher activity compared to substrate Asp-Leu
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?
Asp-Phe + H2O
Asp + Phe
show the reaction diagram
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105% activity compared to substrate Asp-Leu
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?
Asp-Xaa + H2O
Asp + Xaa
show the reaction diagram
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Xaa is an amino acid, substrate specificity, overview
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?
Cys-Leu + H2O
Cys + Leu
show the reaction diagram
Gln-Leu + H2O
Gln + Leu
show the reaction diagram
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9% activity compared to substrate Asp-Leu
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?
Glu-Leu + H2O
Glu + Leu
show the reaction diagram
His-Leu + H2O
His + Leu
show the reaction diagram
Ile-Leu + H2O
Ile + Leu
show the reaction diagram
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2% activity compared to substrate Asp-Leu
-
?
Leu-Cys + H2O
Leu + Cys
show the reaction diagram
Leu-His + H2O
Leu + His
show the reaction diagram
Leu-Ile + H2O
Leu + Ile
show the reaction diagram
Leu-Leu + H2O
Leu + Leu
show the reaction diagram
Leu-Met + H2O
Leu + Met
show the reaction diagram
Leu-Phe + H2O
Leu + Phe
show the reaction diagram
Leu-Tyr + H2O
Leu + Tyr
show the reaction diagram
Leu-Xaa + H2O
Leu + Xaa
show the reaction diagram
Met-Leu + H2O
Met + Leu
show the reaction diagram
O-benzoyl-DL-serinamide + H2O
O-benzoyl-L-serine + NH3
show the reaction diagram
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72% of the activity with DL-beta-methoxy-alpha-amino propionamide, 98.1% enantiomeric excess
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-
?
O-butyl-DL-serinamide + H2O
O-butyl-L-serine + NH3
show the reaction diagram
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51% of the activity with DL-beta-methoxy-alpha-amino propionamide, 96.2% enantiomeric excess
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-
?
O-ethyl-DL-serinamide + H2O
O-ethyl-L-serine + NH3
show the reaction diagram
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83% of the activity with DL-beta-methoxy-alpha-amino propionamide, 98.3% enantiomeric excess
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-
?
O-isopropyl-DL-serinamide + H2O
O-isopropyl-L-serine + NH3
show the reaction diagram
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60% of the activity with DL-beta-methoxy-alpha-amino propionamide, 97.1% enantiomeric excess
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-
?
O-methyl-DL-serinamide + H2O
O-methyl-L-serine + NH3
show the reaction diagram
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99.1% enantiomeric excess
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?
O-propyl-DL-serinamide + H2O
O-propyl-L-serine + NH3
show the reaction diagram
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65% of the activity with DL-beta-methoxy-alpha-amino propionamide, 98.5% enantiomeric excess
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-
?
O-tert-butyl-DL-serinamide + H2O
O-tert-butyl-L-serine + NH3
show the reaction diagram
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45% of the activity with DL-beta-methoxy-alpha-amino propionamide, 94.3% enantiomeric excess
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-
?
Phe-Leu + H2O
Phe + Leu
show the reaction diagram
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7% activity compared to substrate Asp-Leu
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?
Ser-Leu + H2O
Ser + Leu
show the reaction diagram
Thr-Leu + H2O
Thr + Leu
show the reaction diagram
Trp-Leu + H2O
Trp + Leu
show the reaction diagram
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4% activity compared to substrate Asp-Leu
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?
Tyr-Leu + H2O
Tyr + Leu
show the reaction diagram
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6% activity compared to substrate Asp-Leu
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?
Val-Leu + H2O
Val + Leu
show the reaction diagram
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2% activity compared to substrate Asp-Leu
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?
Xaa-Gly-Gly + H2O
Xaa + Gly-Gly
show the reaction diagram
Xaa-Leu + H2O
Xaa + Leu
show the reaction diagram
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Xaa is an amino acid, substrate specificity, overview
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?
Xaa-peptide + H2O
Xaa + peptide
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
Asp-Leu + H2O
Asp + Leu
show the reaction diagram
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best substrate
-
?
Leu-Xaa + H2O
Leu + Xaa
show the reaction diagram
Xaa-peptide + H2O
Xaa + peptide
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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1 mM, 121% of initial activity
Co2+
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1 mM, 128% of initial activity
Mg2+
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1 mM, 132% of initial activity
Mn2+
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1 mM, 122% of initial activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bestatin
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complete inhibition at 1 mM, 37C, after 20 min
Cu2+
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1 mM, 51% of initial activity
EDTA
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reversible by divalent cations
Zn2+
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1 mM, 82% of initial activity
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
20.1
O-benzoyl-DL-serinamide
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pH 9.0, 40C
38.3
O-butyl-DL-serinamide
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pH 9.0, 40C
19.7
O-ethyl-DL-serinamide
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pH 9.0, 40C
29.4
O-isopropyl-DL-serinamide
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pH 9.0, 40C
13.2
O-methyl-DL-serinamide
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pH 9.0, 40C
25.1
O-propyl-DL-serinamide
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pH 9.0, 40C
43.3
O-tert-butyl-DL-serinamide
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pH 9.0, 40C
additional information
additional information
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Km-values for diverse substrates, kinetics
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
950
Asp-Leu-Gly
Salmonella enterica subsp. enterica serovar Typhimurium
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37C, pH 8.5
866
Glu-Leu
Salmonella enterica subsp. enterica serovar Typhimurium
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37C, pH 8.5
918
Leu-Leu
Salmonella enterica subsp. enterica serovar Typhimurium
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37C, pH 8.5
additional information
additional information
Salmonella enterica subsp. enterica serovar Typhimurium
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substrate specificity, overview
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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substrate specificity, overview
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
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6 * 45000, SDS-PAGE; 6 * 46360-46362, mass spectrometry and amino acid sequence determination
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination, overexpression from plasmid, Salmonella typhimurium strain TN5179
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expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
K310V
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site-directed mutagenesis, more than 200fold reduction in the rate of Asp-Leu hydrolysis with no decrease in the rate of Leu-Leu hydrolysis
R402W
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site-directed mutagenesis, reduction in the rate of Asp-Leu hydrolysis with no decrease in the rate of Leu-Leu hydrolysis
additional information
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gene sequence works as functional promoter
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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enzyme can be used for synthesis of alkoxy-serines from DL-beta-alkoxy-alpha-amino propionamides
Show AA Sequence (1367 entries)
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