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Information on EC 3.4.11.2 - membrane alanyl aminopeptidase and Organism(s) Manduca sexta and UniProt Accession P91885

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.2 membrane alanyl aminopeptidase
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This record set is specific for:
Manduca sexta
UNIPROT: P91885 not found.
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Word Map
The taxonomic range for the selected organisms is: Manduca sexta
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Release of an N-terminal amino acid, Xaa-/-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide
Synonyms
aminopeptidase, aminopeptidase n, dipeptidase, aminopeptidase m, alanine aminopeptidase, apn/cd13, gp150, aminopeptidase-n, anpep, alanyl aminopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alanine aminopeptidase
-
-
-
-
alanine-specific aminopeptidase
-
-
-
-
alanyl aminopeptidase
-
-
-
-
Alpha-aminoacylpeptide hydrolase
-
-
-
-
amino-oligopeptidase
-
-
-
-
aminopeptidase M
-
-
-
-
aminopeptidase M II
-
-
-
-
aminopeptidase N
aminopeptidase, microsomal
-
-
-
-
APN1
-
-
-
-
APN2
-
-
-
-
CD13
-
-
-
-
CryIA(C) receptor
-
-
-
-
GP 130
-
-
-
-
GP150
-
-
-
-
L-alanine aminopeptidase
-
-
-
-
Leukemia antigen CD13
-
-
-
-
Lys-AP
-
-
-
-
Lysyl aminopeptidase
-
-
-
-
Membrane glycoprotein H11
-
-
-
-
Membrane protein p161
-
-
-
-
Microsomal aminopeptidase
-
-
-
-
Myeloid plasma membrane glycoprotein CD13
-
-
-
-
particle-bound aminopeptidase
-
-
-
-
PATHWAY SOURCE
PATHWAYS
CAS REGISTRY NUMBER
COMMENTARY hide
9054-63-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
aminoacyl peptide
?
show the reaction diagram
-
-
-
?
insecticidal crystal protein
?
show the reaction diagram
from Bacillus thuringensis, binds to aminopeptidase N
-
-
?
Leu-p-nitroanilide + H2O
Leu + p-nitroaniline
show the reaction diagram
-
-
?
additional information
?
-
-
the enzyme binds the Cry1Ac toxin from Bacillus thuringiensis
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
aminoacyl peptide
?
show the reaction diagram
-
-
-
?
insecticidal crystal protein
?
show the reaction diagram
from Bacillus thuringensis, binds to aminopeptidase N
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
brush border membrane
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AMPN_MANSE
942
0
106780
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
SDS-PAGE
120000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
side-chain modification
-
glycoprotein
-
determination of highly fucosylated N-linked glycans possessing difucosylated cores and Fucalpha(1-3)GlcNAc antennae, N-glycosylation sites are residues N295, N623, and N752, the predominant oligosaccharide bound to N609 is the paucimannoside N-glyca Man3GlcNAc2, detailed overview
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by preparation of brush border membrane vesicles from whole larvae, anion exchange and hydrophobic interaction chromatography, or separation by affinity binding to Cry1Ac toxin
-
native enzyme from brush border membrane vesicles
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
Bacillus thuringiensis Cry1Ab toxin binds to cadherin through loops 2 and 3 of domain II promoting the formation of the pre-pore inducing some structural changes, then the pre-pore interacts with aminopeptidese N through beta-16 of domain III promoting membrane insertion of the toxin and cell death
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Denholf, P.; Hendrickx, K.; Van Damme, J.; Jansens, S.; Peferoen, M.; Degheele, D.; Van Rie, J.
Cloning and characterization of Manduca sexta and Plutella xylostella midgut aminopeptidase N enzymes related to Bacillus thuringiensis toxin-binding proteins
Eur. J. Biochem.
248
748-761
1997
Manduca sexta (P91885), Manduca sexta, Plutella xylostella (P91887), Plutella xylostella
Manually annotated by BRENDA team
Stephens, E.; Sugars, J.; Maslen, S.L.; Williams, D.H.; Packman, L.C.; Ellar, D.J.
The N-linked oligosaccharides of aminopeptidase N from Manduca sexta: site localization and identification of novel N-glycan structures
Eur. J. Biochem.
271
4241-4258
2004
Manduca sexta
Manually annotated by BRENDA team
Adang, M.J.
Insect aminopeptidase N
Handbook of Proteolytic Enzymes (Barrett, J. ; Rawlings, N. D. ; Woessner, J. F. , eds. )
1
296-299
2004
Bombyx mori, Drosophila melanogaster, Heliothis virescens, Manduca sexta, Plutella xylostella, Tenebrio molitor, Epiphyas postvittana, Helicoverpa punctigera
-
Manually annotated by BRENDA team
Gomez, I.; Arenas, I.; Benitez, I.; Miranda-Rios, J.; Becerril, B.; Grande, R.; Almagro, J.C.; Bravo, A.; Soberon, M.
Specific epitopes of domains II and III of Bacillus thuringiensis Cry1Ab toxin involved in the sequential interaction with cadherin and aminopeptidase-N receptors in Manduca sexta
J. Biol. Chem.
281
34032-34039
2006
Manduca sexta
Manually annotated by BRENDA team