Information on EC 3.4.11.17 - tryptophanyl aminopeptidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.4.11.17
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RECOMMENDED NAME
GeneOntology No.
tryptophanyl aminopeptidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
preferential release of N-terminal tryptophan
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of peptide bond
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exopeptidase, N-terminus, amino acid
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CAS REGISTRY NUMBER
COMMENTARY hide
76689-19-5
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-amide + H2O
Ala + NH3
show the reaction diagram
Asn + H2O
Asp + NH3
show the reaction diagram
citrulline + H2O
? + ?
show the reaction diagram
-
-
-
ir
Gln + H2O
Glu + NH3
show the reaction diagram
His-Ala + H2O
His + Ala
show the reaction diagram
-
-
-
ir
His-Gly + H2O
His + Gly
show the reaction diagram
-
-
-
ir
His-Leu + H2O
His + Leu
show the reaction diagram
-
-
-
ir
His-Phe + H2O
His + Phe
show the reaction diagram
-
-
-
ir
L-citrulline + H2O
(2S)-2-amino-5-(carboxyamino)pentanoic acid + NH3
show the reaction diagram
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22% of the activity with Trp-amide
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-
?
Leu-Ala + H2O
Leu + Ala
show the reaction diagram
-
-
-
ir
Leu-amide + H2O
Leu + NH3
show the reaction diagram
Leu-Gly + H2O
Leu + Gly
show the reaction diagram
-
-
-
ir
Leu-Gly-Gly + H2O
Leu + Gly-Gly
show the reaction diagram
-
-
-
ir
Leu-Phe + H2O
Leu + Phe
show the reaction diagram
-
-
-
ir
Leu-Trp + H2O
Leu + Trp
show the reaction diagram
-
-
-
ir
Met-amide + H2O
Met + NH3
show the reaction diagram
Phe-amide + H2O
Phe + NH3
show the reaction diagram
Phe-Leu + H2O
Phe + Leu
show the reaction diagram
-
-
-
ir
Ser-amide + H2O
Ser + NH3
show the reaction diagram
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7% of the activity with Trp-amide
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?
Trp-Ala + H2O
Trp + Ala
show the reaction diagram
-
-
-
ir
Trp-amide + H2O
Trp + NH3
show the reaction diagram
Trp-Glu + H2O
Trp + Glu
show the reaction diagram
-
-
-
ir
Trp-Gly + H2O
Trp + Gly
show the reaction diagram
-
-
-
ir
Trp-Leu + H2O
Trp + Leu
show the reaction diagram
-
-
-
ir
Trp-Phe + H2O
Trp + Phe
show the reaction diagram
-
-
-
ir
Trp-Trp + H2O
Trp + Trp
show the reaction diagram
-
-
-
ir
Trp-Tyr + H2O
Trp + Tyr
show the reaction diagram
-
-
-
ir
Tyr-amide + H2O
Tyr + NH3
show the reaction diagram
Val-Ala + H2O
Val + Ala
show the reaction diagram
-
-
-
ir
Val-amide + H2O
Val + NH3
show the reaction diagram
Val-Gly-Gly + H2O
Val + Gly-Gly
show the reaction diagram
-
-
-
ir
additional information
?
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generally relatively high affinity towards peptides with N-terminal Trp
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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FgaPT1 shows 144.2% relative activity at 5 mM Ca2+, FtmPT1 shows 144.1% relative activity at 5 mM Ca2+
K+
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FgaPT1 shows 117.9% relative activity at 5 mM Co2+
additional information
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7-DMATS is not activated by 5 mM Ca2+, K+, or Mg2+. CdpNPT is not activated by 5 mM Mg2+ or K+. 7-DMATS, FgaPT1, CdpNPT, and FtmPT1 are not influenced by Na+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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27% inhibition at 1 mM
2,2'-dipyridyl
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alpha,alpha'-dipyridyl
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53% inhibition at 1 mM
Ca2+
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CdpNPT shows 69.2% relative activity at 5 mM Ca2+
Co2+
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7-DMATS, FgaPT1, and CdpNPT show 10.2%, 32.3%, and 46.9% relative activity at 5 mM Co2+, respectively
EDTA
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CdpNPT and FtmPT1 are completely inhibited by 5 mM EDTA, 7-DMATS and FgaPT1 show 11.6% and 43.1% relative activity at 5 mM EDTA
Fe2+
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CdpNPT and FtmPT1 show 34.2% and 42.8% relative activity at 5 mM Fe2+, 7-DMATS and FgaPT1 are completely inhibited by 5 mM Fe2+
K+
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FtmPT1 shows 86.5% relative activity at 5 mM K+
Mn2+
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7-DMATS shows 84.0% relative activity at 5 mM Mn2+, FgaPT1 shows 69.7% relative activity at 5 mM Mn2+
monoiodoacetate
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25% inhibition at 1 mM
N-ethylmaleimide
Zn2+
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7-DMATS, FgaPT1, CdpNPT, and FtmPT1 are completely inhibited by 5 mM Zn2+
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ATP
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7-DMATS, FgaPT1, CdpNPT, and FtmPT1 show 151.6%, 145%, 243.6%, and 149.4% relative activity at 5 mM ATP, respectively
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.6
Trp-amide
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1
N-ethylmaleimide
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 45
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.7
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isoelectric focusing
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
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4 * 68000, SDS-PAGE
270000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 68000, SDS-PAGE
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 8.5
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20 h, stable at room temperature
665098
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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heating above causes rapid inactivation
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis