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Information on EC 3.4.11.1 - leucyl aminopeptidase and Organism(s) Schizosaccharomyces pombe and UniProt Accession Q09735

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EC Tree
     3 Hydrolases
         3.4 Acting on peptide bonds (peptidases)
             3.4.11 Aminopeptidases
                3.4.11.1 leucyl aminopeptidase
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: Q09735 not found.
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Word Map
The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
release of an N-terminal amino acid, Xaa-/-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid mides and methyl esters are also readily hydrolysed, but rates on arylamides are exceedingly low
Synonyms
leucine aminopeptidase, erap2, leucyl aminopeptidase, leucine amino peptidase, l-leucine aminopeptidase, leucinaminopeptidase, pilsap, leucine aminopeptidase 3, leucylaminopeptidase, adipocyte-derived leucine aminopeptidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
leucyl aminopeptidase yspII
-
Aminopeptidase A/I
-
-
-
-
cathepsin III
-
-
-
-
cytosol aminopeptidase
-
-
-
-
DR57
-
-
-
-
FTBL proteins
-
-
-
-
L-leucine aminopeptidase
-
-
-
-
LAP
-
-
-
-
leucinamide aminopeptidase
-
-
-
-
leucinaminopeptidase
-
-
-
-
leucine aminopeptidase
-
-
-
-
Leucyl aminopeptidase
-
-
-
-
leucyl peptidase
-
-
-
-
peptidase S
-
-
-
-
proline aminopeptidase
-
-
-
-
Prolyl aminopeptidase
-
-
-
-
proteins, specific or class, FTBL
-
-
-
-
PATHWAY SOURCE
PATHWAYS
CAS REGISTRY NUMBER
COMMENTARY hide
9001-61-0
-
90119-07-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-4-nitroanilide + H2O
Ala + 4-nitroaniline
show the reaction diagram
-
-
-
?
Arg-4-nitroanilide + H2O
Arg + 4-nitroaniline
show the reaction diagram
-
-
-
?
Leu-4-nitroanilide + H2O
Leu + 4-nitroaniline
show the reaction diagram
-
-
-
?
Lys-4-nitroanilide + H2O
Lys + 4-nitroaniline
show the reaction diagram
-
-
-
?
Met-4-nitroanilide + H2O
Met + 4-nitroaniline
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
enhances activity
Mn2+
maximal (5fold) activation at 0.5 mM or higher
Ni2+
enhances activity
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
1 mM, 38% inhibition
bestatin
100 ng/ml, 29% inhibition, competitive
Chloroquine
1 mM, 70% inhibition
EDTA
1 mM, 56% inhibition
leupeptin
0.01 mg/ml, 12% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.7
Arg-4-nitroanilide
pH 8.5, 37°C
4.5
Leu-4-nitroanilide
pH 8.5, 37°C
3.6
Lys-4-nitroanilide
pH 8.5, 37°C
4.4
Met-4-nitroanilide
pH 8.5, 37°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
320000
gel filtration
54000
6 * 54000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
6 * 54000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Herrera-Camacho, I.; Rosas-Murrieta, N.H.; Rojo-Dominguez, A.; Millan, L.; Reyes-Leyva, J.; Santos-Lopez, G.; Suarez-Rendueles, P.
Biochemical characterization and structural prediction of a novel cytosolic leucyl aminopeptidase of the M17 family from Schizosaccharomyces pombe
FEBS J.
274
6228-6240
2007
Schizosaccharomyces pombe (Q09735), Schizosaccharomyces pombe
Manually annotated by BRENDA team