Information on EC 3.3.2.B1 - 2-amino-2-deoxyisochorismate hydrolase

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The expected taxonomic range for this enzyme is: Pseudomonas

EC NUMBER
COMMENTARY hide
3.3.2.B1
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
2-amino-2-deoxyisochorismate hydrolase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate + H2O = (5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid + pyruvate
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate pyruvate-hydrolase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 2-79
SwissProt
Manually annotated by BRENDA team
strain 2-79
SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate + H2O
(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid + pyruvate
show the reaction diagram
chorismate + H2O
?
show the reaction diagram
-
-
-
?
isochorismate + H2O
2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate
show the reaction diagram
hydrolyzed slightly less efficiently than (5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate (i.e. 2-amino-2-deoxy-isochorismate)
-
-
?
additional information
?
-
4-amino-4-deoxychorismate is barely hydrolyzed
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate + H2O
(5S,6S)-6-amino-5-hydroxycyclohexa-1,3-diene-1-carboxylic acid + pyruvate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.068
(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate
i.e. 2-amino-2-deoxy-isochorismate, pH 6.5
0.983
chorismate
pH 6.5
0.004
isochorismate
pH 6.5
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.3
(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate
Pseudomonas aeruginosa
Q7DC80
i.e. 2-amino-2-deoxy-isochorismate, pH 6.5
1.2
chorismate
Pseudomonas aeruginosa
Q7DC80
pH 6.5
0.2
isochorismate
Pseudomonas aeruginosa
Q7DC80
pH 6.5
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
107
(5S,6S)-6-amino-5-[(1-carboxyethenyl)oxy]cyclohexa-1,3-diene-1-carboxylate
Pseudomonas aeruginosa
Q7DC80
i.e. 2-amino-2-deoxy-isochorismate, pH 6.5
27219
1.2
chorismate
Pseudomonas aeruginosa
Q7DC80
pH 6.5
204
50
isochorismate
Pseudomonas aeruginosa
Q7DC80
pH 6.5
1280
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23074
x * 23074, calculated from sequence
23196
2 * 23196, calculated from sequence
23478
2 * 23478, crystallization, light scattering
49400
crystallization, light scattering
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
2 * 23196, calculated from sequence; 2 * 23478, crystallization, light scattering
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
hanging drops by vapor diffusion method, 1.5 A crystal structure of native PhzD, and the 1.6 A structure of the inactive D38A variant in complex with isochorismate
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
wild-type and mutant enzymes
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D38A
inactive variant
K122A
lower activity than mutant enzyme