Information on EC 3.3.2.14 - 2,4-dinitroanisole O-demethylase

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The expected taxonomic range for this enzyme is: Nocardioides

EC NUMBER
COMMENTARY hide
3.3.2.14
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RECOMMENDED NAME
GeneOntology No.
2,4-dinitroanisole O-demethylase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2,4-dinitroanisole + H2O = methanol + 2,4-dinitrophenol
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
2,4-dinitroanisole degradation
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SYSTEMATIC NAME
IUBMB Comments
2,4-dinitroanisole methanol hydrolase
The enzyme, characterized from the bacterium Nocardioides sp. JS1661, is involved in the degradation of 2,4-dinitroanisole. Unlike other known O-demethylases, such as EC 1.14.99.15, 4-methoxybenzoate monooxygenase (O-demethylating), or EC 1.14.11.32, codeine 3-O-demethylase, it does not require oxygen or electron donors, and produces methanol rather than formaldehyde.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
A0A096ZEC9: 2,4-dinitroanisole hydrolase A, A0A096ZED0: 2,4-dinitroanisole hydrolase B
A0A096ZEC9 and A0A096ZED0
UniProt
Manually annotated by BRENDA team
A0A096ZEC9: 2,4-dinitroanisole hydrolase A, A0A096ZED0: 2,4-dinitroanisole hydrolase B
A0A096ZEC9 and A0A096ZED0
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitroanisole + H2O
methanol + 2,4-dinitrophenol
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,4-dinitroanisole + H2O
methanol + 2,4-dinitrophenol
show the reaction diagram
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
A0A096ZEC9 and A0A096ZED0
pH 7.0, temperature not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
A0A096ZEC9 and A0A096ZED0
assay at
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
A0A096ZEC9 and A0A096ZED0
x * 36000 + y * 34000, SDS-PAGE
36000
A0A096ZEC9 and A0A096ZED0
x * 36000 + y * 34000, SDS-PAGE
234000
A0A096ZEC9 and A0A096ZED0
gel filtration
265000
A0A096ZEC9 and A0A096ZED0
native PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
multimer
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
-
A0A096ZEC9 and A0A096ZED0