Information on EC 3.3.2.13 - chorismatase

Word Map on EC 3.3.2.13
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The expected taxonomic range for this enzyme is: Streptomyces hygroscopicus

EC NUMBER
COMMENTARY hide
3.3.2.13
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RECOMMENDED NAME
GeneOntology No.
chorismatase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chorismate + H2O = (4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Phenylalanine, tyrosine and tryptophan biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
chorismate pyruvate-hydrolase
The enzyme found in several bacterial species is involved in the biosynthesis of macrocyclic polyketides.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chorismate + H2O
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
show the reaction diagram
chorismate + H2O
3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
show the reaction diagram
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strict preference for chorismate
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-
?
additional information
?
-
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isochorismate is not accepted as a substrate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
chorismate + H2O
(4R,5R)-4,5-dihydroxycyclohexa-1(6),2-diene-1-carboxylate + pyruvate
show the reaction diagram
chorismate + H2O
3,4-dihydroxycyclohexa-1,5-dienecarboxylate + pyruvate
show the reaction diagram
-
strict preference for chorismate
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-(2-carboxyethyl)benzoate
competitive inhibitor
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.15 - 0.2
chorismate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
17.6
chorismate
Streptomyces hygroscopicus subsp. ascomyceticus
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in deuterated potassium phosphate buffer (20 mM, pH 7.0), at 20C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.02
3-(2-carboxyethyl)benzoate
wild type enzyme, in 100 mM Tris-HCl (pH 7.0), at 25C
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with 3-(2-carboxyethyl)benzoate, hanging drop vapor diffusion method, using 0.1 M 4-morpholineethanesulfonic acid (pH 6.5) and 20-25% (w/v) polyethylene glycol 4000
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Ni-NTA affinity column chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-RPpL1SL2(DE3) cells
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F226A
the mutant shows 2.5% activity compared to the wild type enzyme
R228A
the mutant shows 224.5% activity compared to the wild type enzyme
Y215F
the mutant shows 12% activity compared to the wild type enzyme