Information on EC 3.3.1.2 - adenosylmethionine hydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.3.1.2
-
RECOMMENDED NAME
GeneOntology No.
adenosylmethionine hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + H2O = L-homoserine + S-methyl-5'-thioadenosine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
thioether hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine hydrolase
Also hydrolyses S-methyl-L-methionine sulfonium salt to dimethyl sulfide and homoserine.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-62-3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cabbage
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + H2O
methylthioadenosine + L-homoserine
show the reaction diagram
S-adenosyl-L-methionine chloride + H2O
? + L-homoserine
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine iodide + H2O
? + L-homoserine
show the reaction diagram
-
-
-
?
S-methylmethionine + H2O
L-homoserine + dimethylsulfide
show the reaction diagram
-
i.e. vitamin U, heat and pH sensitive substrate
dimethylsulfide is volatile and serves as quality marker for cabbage
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + H2O
methylthioadenosine + L-homoserine
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-homocysteine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2
S-adenosyl-L-methionine
-
-
1.5
S-adenosyl-L-methionine chloride
-
-
3
S-adenosyl-L-methionine iodide
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
pH 6 20% of maximal activity pH 8 90% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
inactive above
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5000 - 22000
-
3 peaks at 5 kDa, 10 kDa, and 22 kDa, gel filtration
17000
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
stable below
246556
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
stable for 4 min
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 3 months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
expression in Escherichia coli K12
-
expression in tomato
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
-
dimethylsulfide is volatile and serves as quality marker for cabbage