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Information on EC 3.2.2.B5 - 8-oxoguanine DNA glycosylase and Organism(s) Pyrobaculum aerophilum and UniProt Accession Q8ZVK6

for references in articles please use BRENDA:EC3.2.2.B5
preliminary BRENDA-supplied EC number
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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.B5 8-oxoguanine DNA glycosylase
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This record set is specific for:
Pyrobaculum aerophilum
UNIPROT: Q8ZVK6 not found.
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Word Map
The taxonomic range for the selected organisms is: Pyrobaculum aerophilum
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
DNA containing 8-oxoguanine
+
=
DNA with an abasic site
+
Synonyms
8-oxoguanine dna glycosylase, tvg_rs00315, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
archaeal GO glycosylase
-
Pa-AGOG
bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18)
Pa-AGOG DNA glycosylase
PAE2237
locus name
SYSTEMATIC NAME
IUBMB Comments
DNA glycohydrolase [8-oxoguanine releasing]
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA containing 8-oxoguanine + H2O
DNA with an abasic site + 8-oxoguanine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA containing 8-oxoguanine + H2O
DNA with an abasic site + 8-oxoguanine
show the reaction diagram
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
9.2
calculated from sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the enzyme that is part of the base excision repair (BER) pathway. It protects from oxidative damage by removing the major product of DNA oxidation, 8-oxoguanine, from single- and double-stranded DNA substrates. Bifunctional enzyme that catalyzes the excision of 8-oxoguanine by cleaving the N-glycosylic bond between the base and the deoxyribose moiety (glycosylase activity) and subsequently cleave the DNA backbone (lyase activity, EC 4.2.99.18)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29467
x * 29467, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 29467, calculated from sequence
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of the enzyme and that of its complex with 8-oxo-guanosine at 1.0 and 1.7 A resolution, respectively, are grown by sitting drops method
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D166N
mutant enzyme shows wild-type activity
D172N
D172Q
K140Q
K147Q
activity of mutant enzyme is severely attenuated
Q31E/D218S
almost no glycosylase activity
Q31S
significantly reduced glycosylase activity
W222A
almost no glycosylase activity
W222F
mutant is similarly active as wild type on 8-oxoguanine/C substrates
W69F
mutant is similarly active as wild type on 8-oxoguanine/C substrates
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
78
Tm-value for mutant enzyme W69F
80
15 min, no notable effect on catalytic activity
84
Tm-value for mutant enzyme D172Q
86
Tm-value for mutant enzyme Q31S
88
Tm-value for mutant enzyme W222F
93
Tm-value for wild-type enzyme
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lingaraju, G.M.; Prota, A.E.; Winkler, F.K.
Mutational studies of Pa-AGOG DNA glycosylase from the hyperthermophilic crenarchaeon Pyrobaculum aerophilum
DNA Repair
8
857-864
2009
Pyrobaculum aerophilum (Q8ZVK6), Pyrobaculum aerophilum DSM 7523 (Q8ZVK6)
Manually annotated by BRENDA team
Sartori, A.A.; Lingaraju, G.M.; Hunziker, P.; Winkler, F.K.; Jiricny, J.
Pa-AGOG, the founding member of a new family of archaeal 8-oxoguanine DNA-glycosylases
Nucleic Acids Res.
32
6531-6539
2004
Pyrobaculum aerophilum (Q8ZVK6), Pyrobaculum aerophilum DSM 7523 (Q8ZVK6)
Manually annotated by BRENDA team
Lingaraju, G.M.; Sartori, A.A.; Kostrewa, D.; Prota, A.E.; Jiricny, J.; Winkler, F.K.
A DNA glycosylase from Pyrobaculum aerophilum with an 8-oxoguanine binding mode and a noncanonical helix-hairpin-helix structure
Structure
13
87-98
2005
Pyrobaculum aerophilum (Q8ZVK6), Pyrobaculum aerophilum DSM 7523 (Q8ZVK6)
Manually annotated by BRENDA team