Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.2.2.B5 - 8-oxoguanine DNA glycosylase and Organism(s) Archaeoglobus fulgidus and UniProt Accession O29876

for references in articles please use BRENDA:EC3.2.2.B5
preliminary BRENDA-supplied EC number
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.B5 8-oxoguanine DNA glycosylase
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Archaeoglobus fulgidus
UNIPROT: O29876 not found.
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Archaeoglobus fulgidus
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
DNA containing 8-oxoguanine
+
=
DNA with an abasic site
+
Synonyms
8-oxoguanine dna glycosylase, tvg_rs00315, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8-oxoG DNA glycosylase
-
Afogg enzyme
bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities
SYSTEMATIC NAME
IUBMB Comments
DNA glycohydrolase [8-oxoguanine releasing]
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA containing 8-oxoguanine + H2O
DNA with an abasic site + 8-oxoguanine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA containing 8-oxoguanine + H2O
DNA with an abasic site + 8-oxoguanine
show the reaction diagram
the enzyme excises an oxidatively-damaged form of guanine. Bifunctional enzyme with both DNA glycosylase and apurinic/apyrimidinic lyase activities
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NaCl
catalytic activity is inhibited in buffer more than 100 mM NaCl
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 10
pH 7.5: about 50% of maximal activity, pH 10.0: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
since the Tm-value of the DNA substrates (39-mer) of is 74°C, the activity above 60°C is not convincible. The actual optimum temperature of the enzyme might be higher than 60°C
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 80
40°C: about 60% of maximal activity, 80°C: about 70% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22000
1 * 22000, SDS-PAGE
22639
1 * 22639, calculated from sequenec
23000
gel filtration
23124
1 * 23124, mass spectrophotometry
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
pH 7.5 (20 mM Tris–Cl buffer), denatured irreversibly above 80°C
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
overproduced in Escherichia coli
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
overproduced in Escherichia coli as a maltose binding protein(MBP)-Afogg fusion protein
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chung, J.H.; Suh, M.J.; Park, Y.I.; Tainer, J.A.; Han, Y.S.
Repair activities of 8-oxoguanine DNA glycosylase from Archaeoglobus fulgidus, a hyperthermophilic archaeon
Mutat. Res.
486
99-111
2001
Archaeoglobus fulgidus (O29876), Archaeoglobus fulgidus
Manually annotated by BRENDA team