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Information on EC 3.2.2.16 - methylthioadenosine nucleosidase and Organism(s) Staphylococcus aureus and UniProt Accession Q99TQ0

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.16 methylthioadenosine nucleosidase
IUBMB Comments
Unlike EC 3.2.2.9, adenosylhomocysteine nucleosidase, this plant enzyme has little or no activity with S-adenosyl-L-homocysteine.
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This record set is specific for:
Staphylococcus aureus
UNIPROT: Q99TQ0
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Word Map
The taxonomic range for the selected organisms is: Staphylococcus aureus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
5'-methylthioadenosine/s-adenosylhomocysteine nucleosidase, 5'-methylthioadenosine nucleosidase, mta/sah nucleosidase, methylthioadenosine nucleosidase, mta nucleosidase, bgp protein, methylthioadenosine/s-adenosylhomocysteine nucleosidase, glycosaminoglycan-binding protein, s-adenosylhomocysteine/5'-methylthioadenosine nucleosidase, 5-methylthioadenosine/s-adenosylhomocysteine nucleosidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
5-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
5'-methylthioadenosine nucleosidase
-
-
-
-
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
-
MeSAdo nucleosidase
-
-
-
-
methylthioadenosine/S-adenosylhomocysteine nucleosidase
-
-
MTA nucleosidase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-methyl-5'-thioadenosine + H2O = S-methyl-5-thio-D-ribose + adenine
show the reaction diagram
early dissociative transition state
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
S-methyl-5'-thioadenosine adeninehyrolase
Unlike EC 3.2.2.9, adenosylhomocysteine nucleosidase, this plant enzyme has little or no activity with S-adenosyl-L-homocysteine.
CAS REGISTRY NUMBER
COMMENTARY hide
50812-28-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5'-methylthioadenosine + H2O
5-methylthio-D-ribose + adenine
show the reaction diagram
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-D-ribosyl-L-homocysteine + adenine
show the reaction diagram
-
-
-
ir
S-methyl-5'-thioadenosine + H2O
S-methyl-5-thio-D-ribose + adenine
show the reaction diagram
-
-
-
?
5'-deoxyadenosine + H2O
5-deoxy-D-ribose + adenine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-ribosyl-L-homocysteine + adenine
show the reaction diagram
-
-
-
-
?
S-methyl-5'-thioadenosine + H2O
adenine + S-methyl-5-thio-D-ribose
show the reaction diagram
-
-
-
-
?
S-methyl-5'-thioadenosine + H2O
S-methyl-5-thio-D-ribose + adenine
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-homocysteine + H2O
S-D-ribosyl-L-homocysteine + adenine
show the reaction diagram
-
-
-
ir
S-methyl-5'-thioadenosine + H2O
S-methyl-5-thio-D-ribose + adenine
show the reaction diagram
-
-
-
?
5'-deoxyadenosine + H2O
5-deoxy-D-ribose + adenine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-homocysteine + H2O
S-ribosyl-L-homocysteine + adenine
show the reaction diagram
-
-
-
-
?
S-methyl-5'-thioadenosine + H2O
adenine + S-methyl-5-thio-D-ribose
show the reaction diagram
-
-
-
-
?
S-methyl-5'-thioadenosine + H2O
S-methyl-5-thio-D-ribose + adenine
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(3R,4S)-1-[9-deazaadenin-(9-yl)methyl]-3-hydroxy-4-(methylthiomethyl)pyrrolidine
-
4-chlorophenylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
analysis of Kd value, comparison with inhibition of Neisseria meningitides, Helicobacter pylori, Escherichia coli, Streptococcus pneumoniae and human enzyme
benzylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
analysis of Kd value, comparison with inhibition of Neisseria meningitides, Helicobacter pylori, Escherichia coli, Streptococcus pneumoniae and human enzyme
ethylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
analysis of Kd value, comparison with inhibition of Neisseria meningitides, Helicobacter pylori, Escherichia coli, Streptococcus pneumoniae and human enzyme
methylthio-4'-deaza-1'-aza-2'-deoxy-1'-(9-methylene)-immucillin-A
-
analysis of Kd value, comparison with inhibition of Neisseria meningitides, Helicobacter pylori, Escherichia coli, Streptococcus pneumoniae and human enzyme
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00124
5'-methylthioadenosine
recombinant enzyme, in 50 mM potassium phosphate, pH 7.0
0.0009 - 0.0029
S-methyl-5'-thioadenosine
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00973
5'-methylthioadenosine
recombinant enzyme, in 50 mM potassium phosphate, pH 7.0
7.2 - 10.2
S-methyl-5'-thioadenosine
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2480 - 11300
S-methyl-5'-thioadenosine
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
the deletion of the enzyme gene reduces bacterial clumping ability and results in the decreased biofilm formation. The gene mutation also significantly decreases the amount of eDNA present in the biofilm and the cell autolysis
physiological function
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
biologically active dimer, X-ray crystallography
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with the transition-state analogue formycin A, hanging drop vapour diffusion method, using 50 mM potassium phosphate with 20% (w/v) PEG 8000
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
F104C/C186S
the mutant shows reduced activity compared to the wild type
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superdex 200 gel filtration
Ni-NTA Superflow resin column chromatography and Superdex-200 gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 (DE3) cells
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gutierrez, J.A.; Luo, M.; Singh, V.; Li, L.; Brown, R.L.; Norris, G.E.; Evans, G.B.; Furneaux, R.H.; Tyler, P.C.; Painter, G.F.; Lenz, D.H.; Schramm, V.L.
Picomolar inhibitors as transition-state probes of 5-methylthioadenosine nucleosidases
ACS Chem. Biol.
2
725-734
2007
Escherichia coli, Helicobacter pylori, Neisseria meningitidis, Staphylococcus aureus, Streptococcus pneumoniae
Manually annotated by BRENDA team
Siu, K.K.; Lee, J.E.; Smith, G.D.; Horvatin-Mrakovcic, C.; Howell, P.L.
Structure of Staphylococcus aureus 5-methylthioadenosine/S-adenosylhomocysteine nucleosidase
Acta Crystallogr. Sect. F
64
343-350
2008
Escherichia coli (P0AF14), Escherichia coli, Staphylococcus aureus (Q99TQ0), Staphylococcus aureus
Manually annotated by BRENDA team
Wang, S.; Thomas, K.; Schramm, V.L.
Catalytic site cooperativity in dimeric methylthioadenosine nucleosidase
Biochemistry
53
1527-1535
2014
Escherichia coli (P0AF12), Staphylococcus aureus (Q99TQ0), Staphylococcus aureus, Vibrio cholerae (A5F5R2)
Manually annotated by BRENDA team
Bao, Y.; Li, Y.; Jiang, Q.; Zhao, L.; Xue, T.; Hu, B.; Sun, B.
Methylthioadenosine/S-adenosylhomocysteine nucleosidase (Pfs) of Staphylococcus aureus is essential for the virulence independent of LuxS/AI-2 system
Int. J. Med. Microbiol.
303
190-200
2013
Staphylococcus aureus, Staphylococcus aureus NCTC 8325, Staphylococcus aureus RN4220
Manually annotated by BRENDA team
Bao, Y.; Zhang, X.; Jiang, Q.; Xue, T.; Sun, B.
Pfs promotes autolysis-dependent release of eDNA and biofilm formation in Staphylococcus aureus
Med. Microbiol. Immunol.
204
215-226
2015
Staphylococcus aureus, Staphylococcus aureus NCTC 8325
Manually annotated by BRENDA team