Information on EC 3.2.2.15 - DNA-deoxyinosine glycosylase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.2.15
-
RECOMMENDED NAME
GeneOntology No.
DNA-deoxyinosine glycosylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolyses DNA and polynucleotides, releasing free hypoxanthine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
DNA-deoxyinosine deoxyribohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
68247-62-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
calf
-
-
Manually annotated by BRENDA team
Wistar rats and OXYS rats, the latter showing inherited overgeneration of free radicals
-
-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
a repair enzyme involved in the removal of base damage
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
show the reaction diagram
DNA + H2O
hypoxanthine + ?
show the reaction diagram
dsDNA + H2O
ethenocytosine + ?
show the reaction diagram
-
-
-
-
?
dsDNA + H2O
hypoxanthine + ?
show the reaction diagram
-
efficiency of its excision is poor from Hx:T or Hx:C pair but better from an Hx:G pair
-
-
?
dsDNA + H2O
uracil + ?
show the reaction diagram
-
no activity with ssDNA
-
-
?
hypoxanthine-containing DNA + H2O
hypoxanthine + DNA
show the reaction diagram
-
incision activity is increased with age above 12 months of the Wistar and OXYS rats, relation to content of free radicals, overview
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA
show the reaction diagram
strongest activity on the G/I base pair but no activity detected on the C/I base pair
-
-
?
hypoxanthine-containing oligodeoxynucleotide + H2O
hypoxanthine + oligodeoxynucleotide
show the reaction diagram
-
synthetic mismatched substrate, hyopxanthine-removing, incision activity
-
-
?
polydeoxynucleotides + H2O
hypoxanthine + ?
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + H2O
?
show the reaction diagram
hypoxanthine-containing DNA + H2O
hypoxanthine + DNA
show the reaction diagram
-
incision activity is increased with age above 12 months of the Wistar and OXYS rats, relation to content of free radicals, overview
-
-
?
hypoxanthine-containing double-stranded DNA + H2O
hypoxanthine + double-stranded DNA
show the reaction diagram
Q5SJ65
strongest activity on the G/I base pair but no activity detected on the C/I base pair
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
-
the enzyme contains Fe-S cluster
KCl
-
activity 4fold stimulated by 0.1 M KCl
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
higher concentrations, above 10 mM CaCl2
DNA
-
markedly inhibited by low concentrations of native DNA
dsDNA
-
AP-site containing
-
KCl
-
2fold inhibited
Mg2+
-
higher concentrations, above 20 mM MgCl2
NaCl
-
70% inhibited by addition of 0.1 M NaCl
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0005
poly(dAdT)
-
-
-
0.0009
poly-dC
-
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
activity at different ages of both rat races
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2 - 7.8
-
-
7.5
-
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
assay at
60
-
10 min, stable below
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
SDS-PAGE
37000
-
SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56
-
half-life of 3 min when heated at 56°C
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
fully active in presence of 1-5 mM EDTA
-
relatively sensitive to inhibition by neutral salts
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially
-
partially, preparation of mitochondria from liver
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BH214 cells
overexpression in Escherichia coli
-