Information on EC 3.2.2.11 - beta-aspartyl-N-acetylglucosaminidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.2.11
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RECOMMENDED NAME
GeneOntology No.
beta-aspartyl-N-acetylglucosaminidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
1-beta-aspartyl-N-acetyl-D-glucosaminylamine + H2O = L-asparagine + N-acetyl-D-glucosamine
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of N-glycosyl bond
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-
-
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SYSTEMATIC NAME
IUBMB Comments
1-beta-aspartyl-N-acetyl-D-glucosaminylamine L-asparaginohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9027-31-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
human
-
-
Manually annotated by BRENDA team
snail, L.
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-
Manually annotated by BRENDA team
sheep
-
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Manually annotated by BRENDA team
rat
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,2-dideoxy-1-[L-beta-aspartamido]-2-acetamido-beta-D-glucopyranose + H2O
?
show the reaction diagram
-
-
-
-
?
1-beta-aspartyl-2-acetamido-1,2-dideoxy-D-glucosylamine + H2O
N-acetylglucosamine + asparagine
show the reaction diagram
glycopeptide + H2O
?
show the reaction diagram
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-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycopeptide + H2O
?
show the reaction diagram
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-
-
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
630 units/g protein, eluate of starch block after electrophoresis
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
-
activity near the maximum, completely inactive at pH 4.4
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
ultracentrifugation
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
3-5 min at 100°C, activity lost
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
stable to lyophilization and freezing after butanol extraction
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C stable for several months
-
4°C, pH 2-10, several days
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
zone electrophoresis on starch block
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