Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + D-mannose
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
beta-D-Glc-(1->3)-beta-D-Glc + H2O
beta-D-glucose
i.e. laminaribiose, beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6)
-
-
?
beta-D-Glc-(1->4)-beta-D-Glc + H2O
beta-D-glucose
i.e. cellobiose, beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6)
-
-
?
beta-D-Glc-(1->6)-beta-D-Glc + H2O
beta-D-glucose
beta-linked glucose dimers are poorly hydrolyzed. The order of preference of His-tagged enzyme is beta-(1->3), beta-(1->4), beta-(1->6)
-
-
?
methyl beta-D-glucopyranoside + H2O
methanol + beta-D-glucose
n-amyl beta-D-glucopyranoside + H2O
1-pentanol + beta-D-glucose
-
-
-
?
n-decyl beta-D-glucopyranoside + H2O
1-decanol + beta-D-glucose
the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains
-
-
?
n-dodecyl beta-D-glucopyranoside + H2O
1-dodecanol + beta-D-glucose
the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains
-
-
?
n-hexyl beta-D-glucopyranoside + H2O
1-hexanol + beta-D-glucose
-
-
-
?
n-nonyl beta-D-glucopyranoside + H2O
1-nonanol + beta-D-glucose
-
-
-
?
n-octyl beta-D-glucopyranoside + H2O
1-octanol + beta-D-glucose
-
-
-
?
n-undecyl beta-D-glucopyranoside + H2O
1-undecanol + beta-D-glucose
the best substrates for the His-tagged enzyme are beta-D-glucosides with long alkyl chains
-
-
?
salicin + H2O
beta-D-glucose + 2-(hydroxymethyl)phenol
-
-
-
?
additional information
?
-
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
wide substrate specificity belonging to family 1 of glycoside hydrolases classification. The mutant enzyme E324G does not have any activity with the substrate 2-nitrophenyl-beta-D-glucopyranose. However, in the presence of 3 M sodium formate the mutant is reactivated
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
wide substrate specificity belonging to family 1 of glycoside hydrolases classification. The mutant enzyme E324G does not have any activity with the substrate 2-nitrophenyl-beta-D-glucopyranose. However, in the presence of 3 M sodium formate the mutant is reactivated
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
kcat/Km-values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
kcat/Km-values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
kcat/Km values of His-tagged enzyme decreasing in the order: beta-D-glucopyranoside, beta-D-galactopyranoside, beta-D-xylopyranoside, beta-D-mannopyranoside
-
-
?
methyl beta-D-glucopyranoside + H2O
methanol + beta-D-glucose
-
-
-
?
methyl beta-D-glucopyranoside + H2O
methanol + beta-D-glucose
-
-
-
?
additional information
?
-
in the presence of sodium formate buffer pH 4.0 at 75°C the E324G mutant acts as a hyperthermophilic glycosynthase. Though the yield of the reaction does not exceed 10%, it is demonstrated that this could be a general strategy for the preparation of hyperthermophilic glycosynthase
-
-
?
additional information
?
-
preferred substrate are alkyl beta-glycosides
-
-
?
additional information
?
-
the His-tagged enzyme has approximately 50% of the activity of the enzyme without a tag due to interference by the His-tag located at the N-terminus
-
-
?
additional information
?
-
in the presence of sodium formate buffer pH 4.0 at 75°C the E324G mutant acts as a hyperthermophilic glycosynthase. Though the yield of the reaction does not exceed 10%, it is demonstrated that this could be a general strategy for the preparation of hyperthermophilic glycosynthase
-
-
?
additional information
?
-
the His-tagged enzyme has approximately 50% of the activity of the enzyme without a tag due to interference by the His-tag located at the N-terminus
-
-
?
additional information
?
-
preferred substrate are alkyl beta-glycosides
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
15.11 - 39.47
2-nitrophenyl beta-D-glucopyranoside
1.3
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 90°C
0.35
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 90°C
0.14
4-nitrophenyl beta-D-mannopyranoside
pH 6.0, 90°C
0.1
4-nitrophenyl beta-D-xylopyranoside
pH 6.0, 90°C
138.23
beta-D-Glc-(1->3)-beta-D-Glc
pH 6.0, 90°C
1698.18
beta-D-Glc-(1->4)-beta-D-Glc
pH 6.0, 90°C
40.74
methyl beta-D-glucopyranoside
pH 6.0, 90°C
2.02
n-amyl beta-D-glucopyranoside
pH 6.0, 90°C
0.08
n-decyl beta-D-glucopyranoside
pH 6.0, 90°C
0.03
n-dodecyl beta-D-glucopyranoside
pH 6.0, 90°C
0.54
n-hexyl beta-D-glucopyranoside
pH 6.0, 90°C
0.08
n-nonyl beta-D-glucopyranoside
pH 6.0, 90°C
0.2
n-octyl beta-D-glucopyranoside
pH 6.0, 90°C
0.05
n-undecyl beta-D-glucopyranoside
pH 6.0, 90°C
1.96
Salicin
pH 6.0, 90°C
15.11
2-nitrophenyl beta-D-glucopyranoside
pH 6.5, 50 mM sodium phosphate buffer + 3 M sodium formate, 75°C
30.55
2-nitrophenyl beta-D-glucopyranoside
pH 4.0, 50 mM sodium phosphate buffer, 75°C
39.47
2-nitrophenyl beta-D-glucopyranoside
pH 4.0, 200 mM sodium phosphate buffer, 75°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.092 - 1.302
2-nitrophenyl beta-D-glucopyranoside
123
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 90°C
79
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 90°C
2
4-nitrophenyl beta-D-mannopyranoside
pH 6.0, 90°C
3
4-nitrophenyl beta-D-xylopyranoside
pH 6.0, 90°C
184
beta-D-Glc-(1->3)-beta-D-Glc
pH 6.0, 90°C
194
beta-D-Glc-(1->4)-beta-D-Glc
pH 6.0, 90°C
35
methyl beta-D-glucopyranoside
pH 6.0, 90°C
31
n-amyl beta-D-glucopyranoside
pH 6.0, 90°C
37
n-decyl beta-D-glucopyranoside
pH 6.0, 90°C
3 - 6
n-dodecyl beta-D-glucopyranoside
pH 6.0, 90°C
33
n-hexyl beta-D-glucopyranoside
pH 6.0, 90°C
39
n-nonyl beta-D-glucopyranoside
pH 6.0, 90°C
34
n-octyl beta-D-glucopyranoside
pH 6.0, 90°C
43
n-undecyl beta-D-glucopyranoside
pH 6.0, 90°C
0.092
2-nitrophenyl beta-D-glucopyranoside
pH 6.5, 50 mM sodium phosphate buffer + 3 M sodium formate, 75°C
0.592
2-nitrophenyl beta-D-glucopyranoside
pH 4.0, 50 mM sodium phosphate buffer, 75°C
1.302
2-nitrophenyl beta-D-glucopyranoside
pH 4.0, 200 mM sodium phosphate buffer, 75°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.006 - 0.033
2-nitrophenyl beta-D-glucopyranoside
94.34
4-nitrophenyl beta-D-galactopyranoside
pH 6.0, 90°C
225.67
4-nitrophenyl beta-D-glucopyranoside
pH 6.0, 90°C
14.6
4-nitrophenyl beta-D-mannopyranoside
pH 6.0, 90°C
31.83
4-nitrophenyl beta-D-xylopyranoside
pH 6.0, 90°C
1.33
beta-D-Glc-(1->3)-beta-D-Glc
pH 6.0, 90°C
0.11
beta-D-Glc-(1->4)-beta-D-Glc
pH 6.0, 90°C
0.85
methyl beta-D-glucopyranoside
pH 6.0, 90°C
15.11
n-amyl beta-D-glucopyranoside
pH 6.0, 90°C
469.62
n-decyl beta-D-glucopyranoside
pH 6.0, 90°C
1152.9
n-dodecyl beta-D-glucopyranoside
pH 6.0, 90°C
60.28
n-hexyl beta-D-glucopyranoside
pH 6.0, 90°C
471.57
n-nonyl beta-D-glucopyranoside
pH 6.0, 90°C
170.7
n-octyl beta-D-glucopyranoside
pH 6.0, 90°C
944.37
n-undecyl beta-D-glucopyranoside
pH 6.0, 90°C
22.2
Salicin
pH 6.0, 90°C
0.006
2-nitrophenyl beta-D-glucopyranoside
pH 6.5, 50 mM sodium phosphate buffer + 3 M sodium formate, 75°C
0.019
2-nitrophenyl beta-D-glucopyranoside
pH 4.0, 50 mM sodium phosphate buffer, 75°C
0.033
2-nitrophenyl beta-D-glucopyranoside
pH 4.0, 200 mM sodium phosphate buffer, 75°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Perugino, G.; Falcicchio, P.; Michela Corsaro, M.; Matsui, I.; Parrilli, M.; Rossi, M.; Moracci, M.
Preparation of a glycosynthase from the beta-glycosidase of the Archaeon Pyrococcus horikoshii
Biocatal. Biotransform.
24
23-29
2006
Pyrococcus horikoshii (O58104), Pyrococcus horikoshii OT-3 (O58104)
-
brenda
Matsui, I.; Sakai, Y.; Matsui, E.; Kikuchi, H.; Kawarabayasi, Y.; Honda, K.
Novel substrate specificity of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii
FEBS Lett.
467
195-200
2000
Pyrococcus horikoshii (O58104), Pyrococcus horikoshii OT-3 (O58104)
brenda
Akiba, T.; Nishio, M.; Matsui, I.; Harata, K.
X-ray structure of a membrane-bound beta-glycosidase from the hyperthermophilic archaeon Pyrococcus horikoshii
Proteins
57
422-431
2004
Pyrococcus horikoshii (O58104), Pyrococcus horikoshii OT-3 (O58104)
brenda