Information on EC 3.2.1.B35 - Thermococcus kodakaraensis beta-glycosidase

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The expected taxonomic range for this enzyme is: Thermococcus kodakarensis

EC NUMBER
COMMENTARY hide
3.2.1.B35
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Thermococcus kodakaraensis beta-glycosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Exo-type beta-glycosidase activity with cellobiose, cellotriose, cellotetraose or cellopentaose. In vitro it shows high catalytic efficiency with 4-nitrophenyl beta-D-glucopyranoside or 4-nitrophenyl beta-D-mannopyranoside, and lower catalytic efficiency with 4-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-xylopyranoside
show the reaction diagram
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-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucopyranose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
4-nitrophenyl beta-D-mannopyranoside + H2O
4-nitrophenol + beta-D-mannopyranose
show the reaction diagram
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
show the reaction diagram
-
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
-
-
-
?
cellopentaose + H2O
5 D-glucose
show the reaction diagram
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-
-
?
cellotetraose + H2O
4 D-glucose
show the reaction diagram
-
-
-
?
cellotriose + H2O
3 D-glucose
show the reaction diagram
-
-
-
?
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.94 - 1.29
4-nitrophenyl beta-D-fucopyranoside
34.6
4-nitrophenyl beta-D-galactopyranoside
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pH 6.5, 80C
1.79 - 9.23
4-nitrophenyl beta-D-glucopyranoside
0.254 - 8.37
4-nitrophenyl beta-D-mannopyranoside
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
322.3 - 323.4
4-nitrophenyl beta-D-fucopyranoside
3270
4-nitrophenyl beta-D-galactopyranoside
Thermococcus kodakarensis
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pH 6.5, 80C
227.6 - 1650
4-nitrophenyl beta-D-glucopyranoside
20.69 - 243
4-nitrophenyl beta-D-mannopyranoside
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
250.7 - 342.8
4-nitrophenyl beta-D-fucopyranoside
5389
94
4-nitrophenyl beta-D-galactopyranoside
Thermococcus kodakarensis
-
pH 6.5, 80C
999
36.9 - 922
4-nitrophenyl beta-D-glucopyranoside
510
22 - 957
4-nitrophenyl beta-D-mannopyranoside
2120
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60 - 100
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60C: about 40% of maximal activity, 95-100C: optimum
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
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x * 56000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 56000, SDS-PAGE
dimer
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wild-type and mutant enzymes (Q77R, D206N, D206Q, E207S and E399S)
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
90
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half-life: more than 22 h
100
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half-life: less than 1 h
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D206N
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mutation alters the catalytic turn-over rate for glucosidase and mannosidase activities with fucosidase activity remain unchanged
D206Q
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catalytically inactive mutant enzyme. The extended side chain of D206Q is predicted to affect the substrate binding during catalysis
E207S
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catalytically inactive mutant enzyme
E399S
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catalytically inactive mutant enzyme
Q77R
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catalytically inactive mutant enzyme. Q77R might have made some changes in three dimensional structure due to its electrostatic effect and lost its catalytic activity