Information on EC 3.2.1.B33 - Sulfolobus shibatae beta-glycosidase

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The expected taxonomic range for this enzyme is: Sulfolobus shibatae

EC NUMBER
COMMENTARY hide
3.2.1.B33
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
Sulfolobus shibatae beta-glycosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of beta-glycosidic bonds with broad specificity. It prefers laminaribiose (beta1->3Glc), followed by sophorose (beta1->2Glc), gentiobiose (beta1->6Glc), and cellobiose (beta1->4Glc)
show the reaction diagram
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
cellobiose + H2O
D-glucose + D-glucose
show the reaction diagram
gentiobiose + H2O
D-glucose + D-glucose
show the reaction diagram
lactose + H2O
D-glucose + beta-D-galactose
show the reaction diagram
lactose + H2O
D-glucose + D-galactose
show the reaction diagram
laminaribiose + H2O
D-glucose + D-glucose
show the reaction diagram
sophorose + H2O
D-glucose + D-glucose
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
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no effect by 1-5 mM. Addition of 10 mM of MnCl2 enhances the hydrolysis activity of rSSG up to 23% whereas most metal ions do not show any considerable effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,10-phenanthroline
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5 mM, no inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 5% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
4-chloromercuribenzoate
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5 mM, 94% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 90% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Ba2+
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1 mM, 5% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 6% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Ca2+
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1 mM, 5% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 6% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Cd2+
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no inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 12% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Cu2+
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1 mM, 81% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, 36% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Fe2+
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1 mM, 1% inhibition of 2-nitrophenyl mbeta-D-galactopyranoside hydrolysis, 16% inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Hg2+
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1 mM, complete inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis and 4-nitrophenyl beta-D-glucopyranoside hydrolysis
monoiodoacetate
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5 mM, 3% inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis, no inhibition of 4-nitrophenyl beta-D-glucopyranoside hydrolysis
Zn2+
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1 mM, complete inhibition of 2-nitrophenyl beta-D-galactopyranoside hydrolysis and 4-nitrophenyl beta-D-glucopyranoside hydrolysis
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-mercaptoethanol
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increases of the hydrolysis activity
dithiothreitol
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increases the hydrolysis activity
phenylmethanesulfonyl fluoride
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5 mM, 1.1fold activation
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
292.5
75°C, pH not specified in the publication
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.2 - 6.9
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about 80% of maximal activity
5 - 7
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pH 4.0: apout 5% of maximal activity, pH 5.0: maximal activity, pH 7.0: about 40% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
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assay at
98
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hydrolysis of 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80 - 100
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80°C: about 70% of maximal activity, 100°C: about 80% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57400
MALDI-TOF MS
240000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
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the enzyme retains 50% of the initial activity towards 2-nitrophenyl beta-D-galactopyranoside or 4-nitrophenyl beta-D-glucopyranoside after 146 h and 127 h of incubation in phosphate (pH 7.2) or acetate (pH 5.5) buffers. The half-lifes of the enzyme activity at this temperature are decreased after preincubation in acetate buffer (pH 5.5) up to 32 and 27 h, respectively
75
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half-life: 15 h
80
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5 h, in 0.1 M acetate buffer (pH 5.5), the enzyme retained about 67% of the initial activity
95
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half-life: 3.9 min
98
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half-life at pH 7.2: 54 min, half-life at pH 5.5: 30 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
food industry