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Information on EC 3.2.1.B31 - beta-rutinosidase

for references in articles please use BRENDA:EC3.2.1.B31
preliminary BRENDA-supplied EC number
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This record set is specific for:
UNIPROT: Q51723
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The expected taxonomic range for this enzyme is: Eukaryota, Archaea
Reaction Schemes
Synonyms
fhg i, beta-rutinosidase, flavonol 3-glucosidase, more
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
isoquercitrin + H2O
quercetin + beta-D-glucose
show the reaction diagram
best substrate
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
show the reaction diagram
-
-
-
?
rutin + H2O
quercetin + rutinose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
isoquercitrin + H2O
quercetin + beta-D-glucose
show the reaction diagram
best substrate
-
-
?
quercitrin + H2O
quercetin + L-rhamnose
show the reaction diagram
-
-
-
?
rutin + H2O
quercetin + rutinose
show the reaction diagram
-
-
-
?
additional information
?
-
no or poor activity with 4-nitrophenyl alpha-D-glucopyranoside and 4-nitrophenyl alpha-L-rhamnopyranoside, no activity with rutinose, maltose, and turanose
-
-
?
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-methyl-1-butanol
-
Isopropyl alcohol
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
isoquercitrin
pH 5.0, 09°C, recombinant enzyme
0.07
quercitrin
pH 5.0, 09°C, recombinant enzyme
0.34
rutin
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
53.3
isoquercitrin
pH 5.0, 09°C, recombinant enzyme
0.22
quercitrin
pH 5.0, 09°C, recombinant enzyme
0.026 - 0.027
rutin
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
303.3
isoquercitrin
pH 5.0, 09°C, recombinant enzyme
2.33
quercitrin
pH 5.0, 09°C, recombinant enzyme
0.075 - 0.078
rutin
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.23
substrate quercitrin, pH 5.0, 09°C, recombinant enzyme
220
substrate isoquercitrin, pH 5.0, 09°C, recombinant enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
homology modelling using crystal structure of Thermosphaera aggregans beta-glucosidase, PDB ID 1QVB, molecular dynamics, molecular docking of rutin or isoquercitrin as a substrate into the predicted active site, overview. An additional L-rhamnose in rutin might disrupt the binding of rutin to the catalytic residues because of its bulky size and hydroxyl groups
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q51723_9EURY
472
0
54665
TrEMBL
-
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75
half-life: 1017 h
80
half-life: 461 h
85
half-life: 286 h
90
half-life: 173 h
95
half-life: 101 h
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant extracellular enzyme from culture supernatant by heat treatment and centrifugation
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA aand amino acid sequence determination and analysis, expression in Escherichia coli strain ER2566, the enzyme is secreted
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nam, H.K.; Hong, S.H.; Shin, K.C.; Oh, D.K.
Quercetin production from rutin by a thermostable beta-rutinosidase from Pyrococcus furiosus
Biotechnol. Lett.
34
483-489
2012
Pyrococcus furiosus (Q51723)
Manually annotated by BRENDA team