Information on EC 3.2.1.B3 - sucrose 6-phosphate hydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.B3
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
sucrose 6-phosphate hydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside + H2O = alpha-D-glucopyranosyl 6-phosphate + beta-D-fructofuranose
show the reaction diagram
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SYSTEMATIC NAME
IUBMB Comments
sucrose-6-phosphate phosphohydrolase
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
encoded by cg2927 gene that corresponds to the scrB gene; ATCC13032 as wild-type strain, deletion mutant
SwissProt
Manually annotated by BRENDA team
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside + H2O
alpha-D-glucose 6-phosphate + beta-D-fructose
show the reaction diagram
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside + H2O + H2O
alpha-D-glucose 6-phosphate + beta-D-fructose
show the reaction diagram
i.e. sucrose 6-phosphate
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?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
show the reaction diagram
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
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?
sucrose 6-phosphate + H2O
alpha-D-glucose 6-phosphate + beta-D-fructose
show the reaction diagram
metabolism of sucrose, alpha-D-glucose 6-phosphate + beta-D-fructose are metabolized via the glycolytic pathway to yeld primary lactic acid
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?
sucrose 6-phosphate + H2O
glucose 6-phosphate + fructose
show the reaction diagram
additional information
?
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no detectable hydrolysis (at 1 mM) of any of the phosphorylated isomers of sucrose, the enzyme fails to hydrolyze other phospho-alpha-glucosides including maltose 6'-phosphate and trehalose 6-phosphate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside + H2O
alpha-D-glucose 6-phosphate + beta-D-fructose
show the reaction diagram
P13522
i.e. sucrose 6-phosphate, important enzyme in the transport and metabolism of dietary sucrose. Streptococcus mutans coordinates the activities of enzyme IIscr and sucrose-6-phosphate hydrolase by transcriptional repressor ScrR binding to the promoter regions of the scr regulon
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?
sucrose + H2O
alpha-D-glucose + beta-D-fructose
show the reaction diagram
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sucrose uptake and catabolism play important roles in colonization of the murine nasopharynx and an accessory role in the infection of the lung, but is dispensable in a model of bacteraemia. The two sucrose uptake systems are differentially expressed such that one is dominant during lung infection while the other is dominant in colonization of the nasopharynx. Sucrose is a major carbon source utilized by Streptococcus pneumoniae during colonization and infection of mucosal surfaces
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?
sucrose 6-phosphate + H2O
alpha-D-glucose 6-phosphate + beta-D-fructose
show the reaction diagram
P27217
metabolism of sucrose, alpha-D-glucose 6-phosphate + beta-D-fructose are metabolized via the glycolytic pathway to yeld primary lactic acid
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?
sucrose 6-phosphate + H2O
glucose 6-phosphate + fructose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
20% stimulation by MgSO4, 5 mM
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
CaCl2 inhibits activity of, 5 mM, 10% inhibition
fructose
reduced to 70% by the presence of 100 mM
Mn2+
MnCl2 inhibits activity of, 5 mM, 26% inhibition
Zn2+
ZnSO4 inhibits activity of, 5 mM, 98% inhibition
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
fructose 6-phosphate
1.5 fold stimulation, 10 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 0.0853
beta-D-fructofuranosyl 6-O-phosphono-alpha-D-glucopyranoside
20.3 - 190
Sucrose
0.04
sucrose 6-phosphate
recombinant protein, pH 6.5, at 30C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
cg2927 gene corresponds to the scrB gene and encodes sucrose 6-phosphate hydrolase, recombinant protein hydrolyzes sucrose 6-phosphate, sucrose 6-phosphate much more effective than sucrose as substrate, deletion mutant does not grow on sucrose as the sole carbon source, growth in the absence of enzyme activity severely decreased when sucrose is present in addition to glucose, fructose or acetate, transcriptional start sites analyzed, sucrose 6-phosphate hydrolase activity increased during growth in fructose- or sucrose-containing media, overexpression increases hydrolase activity sevenfold, no activity in the scrB deletion mutant
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5
assay at, optimum between 6.5 and 7.0 at 30C
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
13% activity at pH 5.0, 45% activity at pH 8.5 if compared to activity at pH 6.5, no activity below pH 3.0
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
temperature optimum of ScrB protein
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli DH5alpha and BL21(DE3), plasmids pGEM-T, pVWEx1, pET16b, recombinant protein and deletion mutant
expression in Escherichia coli