Information on EC 3.2.1.B23 - ginsenosidase type II

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The expected taxonomic range for this enzyme is: Achatina fulica

EC NUMBER
COMMENTARY hide
3.2.1.B23
preliminary BRENDA-supplied EC number
RECOMMENDED NAME
GeneOntology No.
ginsenosidase type II
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Sequential hydrolysis of the 20-O-beta-D-(1->6)-glucopyranosyl bond (leaving a single glucosyl residue at that position) followed by hydrolysis of the 3-O-beta-D-(1->2)-glucopyranosyl bond and the 3-O-beta-D-glucopyranosyl bond in protopanaxadiol-type ginsenosides
show the reaction diagram
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-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ginsenoside Rb1 + H2O
ginsenoside C-K + 3 D-glucose
show the reaction diagram
-
the enzyme hydrolyzes the 20-O-beta-D-(1->6)-glucoside and both glucosidic linkages at 3-C. The enzyme prefers the 20-C, beta-(1->6)-glucosidic bond to the 3-C, beta-(1->2)-glucosidic bond. Ginsenoside Rb1 is firstly converted to ginsenoside Rd, subsequently to ginsenoside F2, and finally to ginsenoside C-K after 24 h of incubation
-
-
?
ginsenoside Rb2 + 2 H2O
ginsenoside C-Y + 2 D-glucose
show the reaction diagram
-
the enzyme hydrolyzes both glucosidic linkages at 3-C. Ginsenoside Rb2 is firstly converted to ginsenoside C-O and subsequently to ginsenoside C-Y
-
-
?
ginsenoside Rb3 + H2O
ginsenoside Mx + 2 D-glucose
show the reaction diagram
-
the enzyme hydrolyzes both glucosidic linkages at 3-C
-
-
?
ginsenoside Rc + H2O
ginsenoside Mc + 2 D-glucose
show the reaction diagram
-
the enzyme hydrolyzes both glucosidic linkages at 3-C
-
-
?
additional information
?
-
-
the enzyme does not cleave the 20-C-beta-D-(6->1)-arabinopyranoside of ginsenoside Rb2, the 20-C-beta-D-(6->1)-xyloside of ginsenoside Rb3, the 20-O-beta-D-(6->1)-arabinofuranoide of ginsenoside Rc. The enzyme also hydrolyses 4-nitrophenyl-beta-D-glycosides, cellobiose, sophorose, methyl beta-glucopyranoside, hexyl beta-D-glucopyranoside, heptyl beta-D-glucopyranoside, decyl beta-D-glucopyranoside, dodecyl beta-D-glucopyranoside
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
162.8
-
pH 5.0, 50°C, substrate: 4-nitrophenyl-beta-D-glucopyranoside
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 6.5
-
pH 4.0: about 40% of maximal activity, pH 6.5: about 50% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
110000
-
2 * 110000, SDS-PAGE
220000
-
native PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 110000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
might be a glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
-
retains more than 82% of its original activity after 24 h at 30°C
679559
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 50
-
8 h, enzyme retains full activity
60
-
stable for 30 min, 72% loss of activity after 30 min
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE