Information on EC 3.2.1.95 - dextran 1,6-alpha-isomaltotriosidase

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The expected taxonomic range for this enzyme is: Brevibacterium fuscum

EC NUMBER
COMMENTARY hide
3.2.1.95
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RECOMMENDED NAME
GeneOntology No.
dextran 1,6-alpha-isomaltotriosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->6)-alpha-D-glucosidic linkages in dextrans, to remove successive isomaltotriose units from the non-reducing ends of the chains
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
6-alpha-D-glucan isomaltotriohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
72561-11-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 0407
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
dextran + H2O
isomaltotriose
show the reaction diagram
isomaltodextrin + H2O
isomaltotriose
show the reaction diagram
-
reduced
-
?
Sephadex G-100 + H2O
?
show the reaction diagram
-
-
-
-
?
Sephadex G-200 + H2O
?
show the reaction diagram
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-
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AgNO3
-
2 mM, 97% inhibition
CuSO4
-
2 mM, 76% inhibition
HgCl2
-
2 mM, complete inhibition
Iodine
-
2 mM, complete inhibition
N-bromosuccinimide
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2 mM, complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
Km: 0.72 mg/ml for the recombinant enzyme and 0.26 mg/ml for the native enzyme
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
21.5
-
recombinant enzyme
additional information
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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recombinant enzyme
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
-
85% of maximum activity at pH 6.5 and pH 8.0
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
-
x * 68000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.6 - 11
-
37°C, 12 h, stable
208775
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
pH 7.5, 30 min, stable up to
60
-
pH 7.5, 30 min, 30% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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