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EC Tree
The taxonomic range for the selected organisms is: Humicola insolens The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
glucanase, cbh i, cbh ii, cellobiohydrolase ii, cellobiosidase, celluclast 1.5, cellobiohydrolase 1, cel7d, avicelase ii, cellobiohydrolase a,
more
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cellobiohydrolase class II
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1,4-beta-cellobiohydrolase
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-
-
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1,4-beta-D-glucan cellobiohydrolase
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-
-
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1,4-beta-glucan cellobiohydrolase
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-
-
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1,4-beta-glucan cellobiosidase
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-
-
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beta-1,4-glucan cellobiohydrolase
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-
-
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beta-1,4-glucan cellobiosylhydrolase
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-
-
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Beta-1,4-glycanase CEX
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-
-
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Beta-glucancellobiohydrolase
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-
-
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cellobiohydrolase I
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-
-
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cellobiohydrolase II
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-
-
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cellobiohydrolase, exo-
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-
-
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cellobiosidase, 1,4-beta-glucan
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-
-
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exo-beta-1,4-glucan cellobiohydrolase
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-
-
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exo-cellobiohydrolase
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-
-
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exocellobiohydrolase
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-
-
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Cel6A
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-
-
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cellobiohydrolase
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-
-
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hydrolysis of O-glycosyl bond
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-
-
-
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4-beta-D-glucan cellobiohydrolase (non-reducing end)
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avicel + H2O
?
microcrystalline cellulose
-
-
?
cellulose + H2O
cellobiose + ?
-
-
-
?
phosphoric acid swollen cellulose + H2O
cellobiose + ?
-
-
-
?
alpha-cellobiosyl fluoride + H2O
fluoride + cellobiose
-
-
-
-
?
avicel + H2O
cellobiose + ?
-
-
-
-
?
beta-cellobiosyl fluoride + H2O
fluoride + cellobiose
-
-
-
-
?
cellulose + H2O
cellobiose + ?
-
-
-
-
?
cellulose + H2O
cellobiose + D-glucose + cellotriose
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Cel6A is a more endo-processive enzyme, whereas Cel7A is essentially a processive enzyme, both forms show a synergistic effect in the digestion of bacterial cellulose
-
-
?
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cellulose + H2O
cellobiose + ?
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-
-
-
?
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0.77
alpha-cellobiosyl fluoride
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pH 5, 20°C
0.022
beta-cellobiosyl fluoride
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pH 5, 20°C
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0.01
alpha-cellobiosyl fluoride
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pH 5, 20°C
0.071
beta-cellobiosyl fluoride
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pH 5, 20°C
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additional information
wild-type, 4.0 microg glucose reducing sugar equivalent per microg enzyme and min, chimera HJPlus, 10.0 microg glucose reducing sugar equivalent per microg enzyme and min, pH 4.8, 50°C
additional information
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wild-type, 4.0 microg glucose reducing sugar equivalent per microg enzyme and min, chimera HJPlus, 10.0 microg glucose reducing sugar equivalent per microg enzyme and min, pH 4.8, 50°C
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Uniprot
brenda
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GUX6_HUMIN
476
0
51276
Swiss-Prot
Secretory Pathway (Reliability: 1 )
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glycoprotein
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alpha-linked mannose and N-linked glycolysation
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D416A mutant, hanging drop vapor diffusion method, complexed with methyl cellobiosyl-4-thio-beta-cellobioside
wild-type and mutant forms in complex with nonhydrolyzable thio-oligosaccharides, hanging drop vapor diffusion method
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C313S
random mutagenesis, the mutation causes increased thermostability of the mutant enzyme compared to the wild-type, with decreased inactivation, increased maximum Avicel hydrolysis temperature, and improved long time hydrolysis performance
D405A
about 1% enzyme activity
D405N
only small structural changes, about 1% enzyme activity
A385P
-
the mutant shows a T50 value of 64.7°C
M235P
-
the mutant shows a T50 value of 63.1°C
Q390P
-
the mutant shows a T50 value of 66.1°C
S215P
-
the mutant shows a T50 value of 65.6°C
D416A
active center N-terminal loop has a more open conformation
D416A
crystal structure similar to wild-type enzyme, mutant retains about 10% of wild-type activity
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75
-
the wild type enzyme shows a half-life of more than 2.5 min at 75°C and T50 of 65.2°C
90
-
the enzyme is inactivated after 15-minute incubation at 90°C and pH 7.0
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His-Trap column chromatography
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Ni-NTA column chromatography
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expression in Saccharomyces cerevisiae
expressed in Aspergillus oryzae
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expressed in Saccharomyces cerevisiae strain YDR483W BY4742
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detergent
usage in paper-, detergent- and textile industry
paper production
usage in paper-, detergent- and textile industry
synthesis
SCHEMA structure-guided recombination of fungal class II cellobiohydrolases (CBH II cellulases) from Humicola insolens, Hypocrea jecorina and Chaetomium thermophiulum and mathematical modeling yields a collection of highly thermostable CBH II chimeras with more activity than Humicola insolens CBH II after incubation at 63 °C. The total of 15 validated thermostable CBH II enzymes have high sequence diversity, differing from their closest natural homologs at up to 63 amino acid positions. Selected purified thermostable chimeras hydrolyze phosphoric acid swollen cellulose at temperatures 7 to 15°C higher than the parent enzymes. These chimeras also hydrolyze as much or more cellulose than the parent CBH II enzymes in long-time cellulose hydrolysis assays and have pH/activity profiles as broad, or broader than, the parent enzymes. The best chimera with buildung blocks from all three organisms exhibits both relatively high specific activity and high thermostability
textile production
usage in paper-, detergent- and textile industry
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Varrot, A.; Hastrup, S.; Schulein, M.; Davies, G.J.
Crystal structure of the catalytic core domain of the family 6 cellobiohydrolase II, Cel6A, from Humicola insolens, at 1.92 A resolution
Biochem. J.
337
297-304
1999
Humicola insolens
brenda
Varrot, A.; Frandsen, T.P.; Driguez, H.; Davies, G.J.
Structure of the Humicola insolens cellobiohydrolase Cel6A D416A mutant in complex with a non-hydrolysable substrate analogue, methyl cellobiosyl-4-thio-beta-cellobioside, at 1.9.ANG
Acta Crystallogr. Sect. D
D58
2201-2204
2002
Humicola insolens (Q9C1S9)
brenda
Boisset, C.; Fraschini, C.; Schulein, M.; Henrissat, B.; Chanzy, H.
Imaging the enzymatic digestion of bacterial cellulose ribbons reveals the endo character of the cellobiohydrolase Cel6A from Humicola insolens and its mode of synergy with cellobiohydrolase Cel7A
Appl. Environ. Microbiol.
66
1444-1452
2000
Humicola insolens
brenda
Becker, D.; Johnson, K.S.; Koivula, A.; Schulein, M.; Sinnott, M.L.
Hydrolyses of alpha- and beta-cellobiosyl fluorides by Cel6A (cellobiohydrolase II) of Trichoderma reesei and Humicola insolens
Biochem. J.
345
315-319
2000
Humicola insolens, Trichoderma reesei
-
brenda
Varrot, A.; Frandsen, T.P.; von Ossowski, I.; Boyer, V.; Cottaz, S.; Driguez, H.; Schulein, M.; Davies, G.J.
Structural basis for ligand binding and processivity in cellobiohydrolase Cel6A from Humicola insolens
Structure
11
855-864
2003
Humicola insolens (Q9C1S9)
brenda
Heinzelman, P.; Snow, C.D.; Smith, M.A.; Yu, X.; Kannan, A.; Boulware, K.; Villalobos, A.; Govindarajan, S.; Minshull, J.; Arnold, F.H.
SCHEMA recombination of a fungal cellulase uncovers a single mutation that contributes markedly to stability
J. Biol. Chem.
284
26229-26233
2009
Phanerodontia chrysosporium, Trichoderma reesei (P07987), Humicola insolens (Q9C1S9), Humicola insolens
brenda
Wu, I.; Heel, T.; Arnold, F.H.
Role of cysteine residues in thermal inactivation of fungal Cel6A cellobiohydrolases
Biochim. Biophys. Acta
1834
1539-1544
2013
Humicola insolens, Trichoderma reesei
brenda
Wu, I.; Arnold, F.H.
Engineered thermostable fungal Cel6A and Cel7A cellobiohydrolases hydrolyze cellulose efficiently at elevated temperatures
Biotechnol. Bioeng.
110
1874-1883
2013
Thermochaetoides thermophila, Humicola insolens, Trichoderma reesei
brenda
Heinzelman, P.; Snow, C.D.; Wu, I.; Nguyen, C.; Villalobos, A.; Govindarajan, S.; Minshull, J.; Arnold, F.H.
A family of thermostable fungal cellulases created by structure-guided recombination
Proc. Natl. Acad. Sci. USA
106
5610-5615
2009
Trichoderma reesei (P07987), Thermochaetoides thermophila (Q4JQF8), Humicola insolens (Q9C1S9), Humicola insolens
brenda