Information on EC 3.2.1.88 - non-reducing end beta-L-arabinopyranosidase

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The expected taxonomic range for this enzyme is: Phlebotomus papatasi

EC NUMBER
COMMENTARY
3.2.1.88
-
RECOMMENDED NAME
GeneOntology No.
non-reducing end beta-L-arabinopyranosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
removal of a terminal beta-L-arabinopyranose residue from the non-reducing end of its substrate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-L-arabinopyranoside non-reducing end beta-L-arabinopyranosidase
The enzyme, which was characterized from dormant seeds of the plant Cajanus cajan (pigeon pea), has been shown to remove the terminal non-reducing beta-L-arabinopyranoside residue from the artificial substrate p-nitrophenyl-beta-L-arabinopyranose [1]. In the presence of methanol the enzyme demonstrates transglycosylase activity, transferring the arabinose moiety to methanol while retaining the anomeric configuration, generating 1-O-methyl-beta-L-arabinopyranose [2].
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
arabinosidase,beta-L-
-
-
-
-
beta-L-arabinosidase
-
-
-
-
beta-L-arabinosidase
-
-
vicianosidase
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
39361-63-2
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Cajanus indicus
-
-
-
Manually annotated by BRENDA team
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
p-nitrophenyl beta-L-arabinoside + H2O
p-nitrophenol + beta-L-arabinose
show the reaction diagram
Cajanus indicus
-
-
-
?
p-nitrophenyl beta-L-arabinoside + H2O
p-nitrophenol + beta-L-arabinose
show the reaction diagram
Cajanus indicus
-
-
-
-
?
4-nitrophenyl-alpha-arabinopyranoside + H2O
?
show the reaction diagram
-
activity measured in whole fly homogenates, substrate concentration of 60 mM in acetate phospate buffer used
-
-
?
additional information
?
-
Cajanus indicus
-
no hydrolysis of: p-nitrophenyl alpha-D-galactoside, p-nitrophenyl alpha-D-fucoside, p-nitrophenyl beta-D-galactoside
-
-
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.8
-
p-nitrophenyl beta-L-arabinoside
Cajanus indicus
-
-
0.83
-
p-nitrophenyl-beta-L-arabinoside
Cajanus indicus
-
-
additional information
-
additional information
Cajanus indicus
-
very small effect of pH in the range 2.5-7.2, no influence of alcohols on Km
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
assay described, activity measured in whole body homogenates of 10 to 15 male and female flies before and after sucrose meals, monitoring by spectroscopy, values of relative activity shown
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
4.6
Cajanus indicus
-
-
5.7
-
-
assay at, pH-optimum estimated for
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
6.5
Cajanus indicus
-
-
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
-
Cajanus indicus
-
assay at
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
Cajanus indicus
-
-
Manually annotated by BRENDA team
-
whole fly homogenates
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25900
-
Cajanus indicus
-
gel filtration
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
6.5
Cajanus indicus
-
30°C, 30 min
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
40
Cajanus indicus
-
-
OXIDATION STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
photooxidation in presence of methylene blue
Cajanus indicus
-
136932
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-
Cajanus indicus
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
comparison of glycolytic and chitinolytic enzyme activities between desert and oasis flies of Phlebotomus papatasi to evaluate potential differences in susceptibility to infection with Leishmania major