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Information on EC 3.2.1.86 - 6-phospho-beta-glucosidase and Organism(s) Erwinia rhapontici and UniProt Accession Q9AI65

for references in articles please use BRENDA:EC3.2.1.86
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IUBMB Comments
Also hydrolyses several other phospho-beta-D-glucosides, but not their non-phosphorylated forms.
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This record set is specific for:
Erwinia rhapontici
UNIPROT: Q9AI65
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Word Map
The taxonomic range for the selected organisms is: Erwinia rhapontici
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
phospho-beta-glucosidase, 6-phospho-alpha-glucosidase, phospho-alpha-glucosidase, 6-phospho-beta-glucosidase, gan1d, lacg2, spy1599, lacg1, bgla-2, bgl-2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6-phospho-alpha-glucosidase
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phospho-alpha-glucosidase
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Cellobiose-6-phosphate hydrolase
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-
-
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phospho-beta-glucosidase
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-
-
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phospho-beta-glucosidase A
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-
-
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phosphocellobiase
-
-
-
-
additional information
the enzyme belongs to the glycsoylhydrolase family GH4. Glycosyl hydrolase family 4, GH4 is exceptional among the 114 families in this enzyme superfamily. Members of GH4 exhibit unusual cofactor requirements for activity, and an essential cysteine residue is present at the active site
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
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SYSTEMATIC NAME
IUBMB Comments
6-phospho-beta-D-glucosyl-(1->4)-D-glucose glucohydrolase
Also hydrolyses several other phospho-beta-D-glucosides, but not their non-phosphorylated forms.
CAS REGISTRY NUMBER
COMMENTARY hide
37205-51-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain DSM448, gene palH
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PALH_ERWRD
453
0
50341
Swiss-Prot
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E173S
site-directed mutagenesis
E173S/I174V
site-directed mutagenesis
I174V
site-directed mutagenesis
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant soluble His-tagged wild-type and mutant PalHs from Escherichia coli by ultracentrifugation, anion exchange chromatography, and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene palH, palH is present in a cluster of nine genes whose products may facilitate the transport, and metabolism of palatinose in this plant pathogen, phylogenetic analysis, expression of soluble His-tagged wild-type and mutant PalHs in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Hall, B.G.; Pikis, A.; Thompson, J.
Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations
Mol. Biol. Evol.
26
2487-2497
2009
Erwinia rhapontici (Q9AI65), Erwinia rhapontici, Erwinia rhapontici DSM 448 (Q9AI65)
Manually annotated by BRENDA team