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EC Tree
The taxonomic range for the selected organisms is: Thermotoga maritima The enzyme appears in selected viruses and cellular organisms
Synonyms
endoxylanase, xylanase a, endo-xylanase, xyn11a, xyn10a, beta-xylanase, endo-1,4-beta-xylanase, gh11 xylanase, xylanase b, xynii,
more
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endo-(1,4)-beta-xylanase
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(1--> 4)-beta-xylan 4-xylanohydrolase
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1,4-beta-D-xylan xylanohydrolase
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1,4-beta-D-xylan xylanohydrolase 22
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1,4-beta-D-xylan-xylanohydrolase
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1,4-beta-xylan xylanohydrolase
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beta-1,4-D-xylanase
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beta-1,4-xylan xylanohydrolase
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beta-1,4-xylanase
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endo-(1--> 4)-beta-xylanase
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endo-1,4-beta-D-xylanase
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endo-1,4-beta-xylanase
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endo-1,4-xylanase
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endo-beta-1,4-xylanase
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xylanase, endo-1,4-
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xylanase
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XynA
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XynB
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4-beta-D-xylan xylanohydrolase
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1,4-beta-D-xylan + H2O
?
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?
beta-1,4-xylan + H2O
?
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?
beta-1,4-xylan + H2O
xylobiose + xylotriose + ?
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from birchwood
the main degradation products
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?
carboxymethylcellulose + H2O
?
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low activity, 1.4% activity compared to xylan
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?
gellan gum + H2O
?
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low activity, 7.3% activity compared to xylan
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?
laminarin + H2O
?
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low activity, 3.4% activity compared to xylan
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?
lichenan + H2O
?
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low activity, 19% activity compared to xylan
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?
oat spelt xylan + H2O
?
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?
xylan + H2O
xylobiose + xylose + ?
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birchwood and soluble oat spelt xylan, endo-acting
main degradation products
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?
xylooligosaccharides + H2O
xylobiose + xylose
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predominantly xylobiose
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?
additional information
?
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substrate specificity, no activity with Avicel, filter paper, starch, amylopectin, pullulan, galactoglucomannan, or galactomannan
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xylan + H2O
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hydrolysis of beta-1,4-glycosidic bonds
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xylan + H2O
?
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from birchwood or oatspelt
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?
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beta-1,4-xylan + H2O
?
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?
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additional information
additional information
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89.7
xylan
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soluble from oat spelt, pH 6.1, 50°C
106.5
xylan
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from birchwood, pH 6.1, 50°C
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1022
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substrate oat spelt xylan, purified recombinant enzyme
1099
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substrate beechwood xylan, purified recombinant enzyme
12
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substrate carboxymethylcellulose, purified recombinant enzyme
164
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substrate lichenan, purified recombinant enzyme
30
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substrate laminarin, purified recombinant enzyme
64
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substrate gellan gum, purified recombinant enzyme
867
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substrate birchwood xylan, purified recombinant enzyme
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65
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recombinant hybrid mutant
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30 - 90
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activity range of mutant hybrid enzyme, profile, overview
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endo-1,4-beta-xylanase A precursor
UniProt
brenda
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most XynA remains cell associated in the outer membrane
brenda
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XynB is detected in the periplasmic fraction and in the culture supernatant
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brenda
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physiological function
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the N-terminal domain A2 is responsible for both the thermostability and substrate-binding capacity of XynA
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119642
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x * 119642, calculated
26000
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x * 26000, mutant hybrid enzyme, SDS-PAGE
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additional information
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enzyme folds into a (beta/alpha)8-barrel structure, and has characteristic clusters of aromatic residues together with a lack of exposed hydrophobic residues, crystallization data
?
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x * 119642, calculated
?
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x * 26000, mutant hybrid enzyme, SDS-PAGE
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in complex with xylotriose and xylobiose
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purified recombinant enzyme, sitting drop vapour diffusion method, 40 mM zinc acetate, 20 mM MES, pH 6.0, 3% ethanol, X-ray diffraction structure determination and preliminary analysis at 2.5 A resolution
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additional information
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improving the thermostability of Trichoderma reesei xylanase 2 by introducing the thermostabilizing domain A2 from Thermotoga maritima XynA into the N-terminal region of the Xyn2 protein
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60
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recombinant mutant hybrid enzyme, 30 min, over 85% activity remaining
additional information
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features that account for the high thermostability of enzyme are clusters of aromatic residues together with a lack of exposed hydrophobic residues
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recombinant His-tagged enzyme from Escherichia coli, 12.5fold to homogeneity by Ni2+-chelate affinity chromatography
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expressed in Escherichia coli
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expression of the hybrid gene in Pichia pastoris under control of the inducible alcohol oxidase 1, AOX1, promoter using the secretion signal sequence from Saccharomyces cerevisiae, subcloning in Escherichia coli strain DH5alpha
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gene xynB, expression as His-tagged enzyme in Escherichia coli BL21
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xylanase (XynB) and alpha-glucuronidase (AguA) from Thermotoga maritima are coproduced in Escherichia coli through dual-promoter and bicistronic constructs
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food industry
xylooligosaccharide derived from enzymatic hydrolysis of biopolymers is of considerable importance in preparing nutritional health oligosaccharides useful in food and pharmaceutical industries. To create added value products from hardwood xylan, xylanase (XynB) and alpha-glucuronidase (AguA) from Thermotoga maritima were co-produced in Escherichia coli through dual-promoter and bicistronic constructs
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Ihsanawati; Kumasaka, T.; Kaneko, T.; Morokuma, C.; Nakamura, S.; Tanaka, N.
Crystallization and preliminary X-ray studies of xylanase 10B from Thermotoga maritima
Acta Crystallogr. Sect. D
59
1659-1661
2003
Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
brenda
Jiang, Z.Q.; Deng, W.; Zhu, Y.P.; Li, L.T.; Sheng, Y.J.; Hayashi, K.
The recombinant xylanase B of Thermotoga maritima is highly xylan specific and produces exclusively xylobiose from xylans, a unique character for industrial applications
J. Mol. Catal. B
27
207-213
2004
Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
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brenda
Yoon, H.; Han, N.S.; Kim, C.H.
Expression of Thermotoga maritima endo-beta-1,4-xylanase gene in E. coli and characterization of the recombinant enzyme
Agric. Chem. Biotechnol.
47
157-160
2004
Thermotoga maritima
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brenda
Ihsanawati, K.; Kumasaka, T.; Kaneko, T.; Morokuma, C.; Yatsunami, R.; Sato, T.; Nakamura, S.; Tanaka, N.
Structural basis of the substrate subsite and the highly thermal stability of xylanase 10B from Thermotoga maritima MSB8
Proteins
61
999-1009
2005
Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
brenda
Berrin, J.G.; Juge, N.
Factors affecting xylanase functionality in the degradation of arabinoxylans
Biotechnol. Lett.
30
1139-150
2008
Bacillus sp. (in: Bacteria), Cellvibrio japonicus, Cellvibrio japonicus (Q59675), Acetivibrio thermocellus (O52780), Streptomyces lividans (P26514), Neocallimastix patriciarum (P29127), Paenibacillus polymyxa (P45796), Cellulomonas fimi (P54865), Aspergillus niger (P55329), Rhodothermus marinus (P96988), Aspergillus nidulans (Q00177), Thermotoga maritima (Q60037), Streptomyces olivaceoviridis (Q7SI98), Thermoclostridium stercorarium (Q8GJ44), Talaromyces funiculosus (Q9HFH0), Trichoderma viride (Q9UVF9)
brenda
Liebl, W.; Winterhalter, C.; Baumeister, W.; Armbrecht, M.; Valdez, M.
Xylanase attachment to the cell wall of the hyperthermophilic bacterium Thermotoga maritima
J. Bacteriol.
190
1350-1358
2008
Thermotoga maritima, Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
brenda
Jun, H.; Bing, Y.; Keying, Z.; Xuemei, D.; Daiwen, C.
Thermostable carbohydrate binding module increases the thermostability and substrate-binding capacity of Trichoderma reesei xylanase 2
New Biotechnol.
26
53-59
2009
Thermotoga maritima, Trichoderma reesei, Trichoderma reesei RUT C-30
brenda
Zhou, T.; Xue, Y.; Ren, F.; Dong, Y.
Antioxidant activity of xylooligosaccharides prepared from Thermotoga maritima using recombinant enzyme cocktail of beta-xylanase and alpha-glucuronidase
J. Carbohydr. Chem.
37
210-224
2018
Thermotoga maritima (Q9WXS5), Thermotoga maritima ATCC 43589 (Q9WXS5)
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brenda