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arabinoxylan + H2O
xylo-oligosaccharide + ?
beechwood xylan + H2O
?
-
-
-
?
beta-1,4-xylan + H2O
xylo-oligosaccharide + ?
birchwood xylan + H2O
?
-
-
-
?
oat spelt xylan + H2O
?
-
-
-
?
Remazol brilliant blue-birchwood xylan + H2O
?
-
-
-
?
rye arabinoxylan + H2O
?
-
-
-
?
1,4-beta-D-xylan + H2O
?
-
-
-
-
?
4-methoxyphenol + H2O
?
weak transxylosylation activity
-
-
?
ascorbic acid + H2O
?
weak transxylosylation activity
-
-
?
azurin-labelled birchwood xylan + H2O
?
-
-
-
-
?
beech wood xylan + H2O
?
strong transxylosylation activity
-
-
?
beechwood xylan + H2O
?
low affinity of the enzyme towards this substrate
-
-
?
beta-1,4-xylan + H2O
xylobiose + xylotriose + ?
caffeic acid + H2O
?
weak transxylosylation activity
-
-
?
catechin + H2O
?
strong transxylosylation activity
-
-
?
catechol + H2O
?
strong transxylosylation activity
-
-
?
DOPA + H2O
?
weak transxylosylation activity
-
-
?
dopamine + H2O
?
weak transxylosylation activity
-
-
?
fructose + H2O
?
weak transxylosylation activity
-
-
?
gallic acid + H2O
?
strong transxylosylation activity
-
-
?
glucose + H2O
?
weak transxylosylation activity
-
-
?
hydroquinone + H2O
?
strong transxylosylation activity
-
-
?
lactose + H2O
?
strong transxylosylation activity
-
-
?
maltose + H2O
?
strong transxylosylation activity
-
-
?
mannitol + H2O
?
weak transxylosylation activity
-
-
?
nutrient broth + H2O
?
strong transxylosylation activity
-
-
?
phloroglucinol + H2O
?
weak transxylosylation activity
-
-
?
pyrogallol + H2O
?
strong transxylosylation activity
-
-
?
resorcinol + H2O
?
weak transxylosylation activity
-
-
?
starch + H2O
?
strong transxylosylation activity
-
-
?
sucrose + H2O
D-fructose + D-glucose
strong transxylosylation activity
-
-
?
wheat arabinoxylan + H2O
?
-
-
-
-
?
xylan + H2O
xylobiose + xylotriose + xylotetraose
the hydrolysis products are mainly xylobiose (57.5% with immobilized enzyme, 36.1% with soluble enzyme) and xylotriose (38.4% with immobilized enzyme, 48.7% with soluble enzyme)
-
-
?
xylan + H2O
xylose + xylotriose + xylotetraose
-
-
-
-
?
xylooligosaccharide + H2O
?
weak transxylosylation activity
-
-
?
xylooligosaccharides + H2O
?
-
-
-
?
xylose + H2O
?
weak transxylosylation activity
-
-
?
1,4-beta-D-xylan + H2O
additional information
-
arabinoxylan + H2O
?
-
-
-
?
arabinoxylan + H2O
?
-
-
-
-
?
arabinoxylan + H2O
xylo-oligosaccharide + ?
-
product analysis, overview
-
?
arabinoxylan + H2O
xylo-oligosaccharide + ?
higher activity with water-unextractable, than-extractable arabinoxylans, overview
product analysis, overview
-
?
beta-1,4-xylan + H2O
?
-
-
-
?
beta-1,4-xylan + H2O
?
from birchwood
-
-
?
beta-1,4-xylan + H2O
xylo-oligosaccharide + ?
-
product analysis, overview
-
?
beta-1,4-xylan + H2O
xylo-oligosaccharide + ?
from birchwood xylan and wheat bran
product analysis, overview
-
?
arabinoxylan + H2O
?
-
-
-
-
?
arabinoxylan + H2O
?
-
the GHF 11 endoxylanase has a higher substrate specificity as compared to GHF 10 endoxylanases
-
-
?
beta-1,4-xylan + H2O
xylobiose + xylotriose + ?
-
-
the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose
-
?
beta-1,4-xylan + H2O
xylobiose + xylotriose + ?
from birchwood
major products with equimolar ratio
-
?
beta-1,4-xylan + H2O
xylobiose + xylotriose + ?
-
from oat spelt or birchwood
the predominant products resulting from xylan and xylooligosaccharide hydrolysis are xylobiose and xylotriose. The enzyme can hydrolyze xylooligosaccharides larger than xylotriose
-
?
1,4-beta-D-xylan + H2O
additional information
-
-
larchwood xylan
-
-
?
1,4-beta-D-xylan + H2O
additional information
-
-
soluble larchwood xylan
-
-
?
additional information
?
-
-
digestion of a (1, 3),(1, 4)-beta-D-linkage sequence xylan from Rhodymenia into 4-linked xylobiose and xylotriose and 3,4-mixed linked oligosaccharides having a degree of polymerization of four or more
-
-
?
additional information
?
-
displays no transxylosylation activity toward xylitol, cellulose, chitin, chitosan, pectin, sorbitol, ribose, phenol, guaiacol, o-nitorphenol, vanilin and alcoholic compounds (methanol, ethanol, ethylene grecol and grecerol)
-
-
?
additional information
?
-
-
AMX-4 xylanase is not active toward other polysaccharides including carboxymethylcellulose, locust bean gum, lichenan, laminarin, and Avicel or toward synthetic substrate derivatives such as 4-nitrophenyl-beta-xylopyranoside, 4-nitrophenyl-beta-mannoside, and 4-nitrophenyl-beta-cellobioside, it can hydrolyze only beta-1,4-xylosidic linkages and has no beta-xylosidase activity
-
-
?
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D11F/R122D
the mutant shows highly decreased sensitivity to inhibitor Triticum aestivum xylanase inhibitor compared to wild-type enzyme
G23R
construction of a XynA mutant with increased pH stability, Computational design-based molecular engineering, overview
Q175K
construction of a XynA mutant with increased pH stability, Computational design-based molecular engineering, overview
T10H
construction of a XynA mutant with increased pH stability, Computational design-based molecular engineering, overview
W9H
construction of a XynA mutant with increased pH stability, Computational design-based molecular engineering, overview
F48C
-
mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme
T44C
-
mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme
T44Y
-
mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme
T87D
-
mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme
Y94C
-
mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme
additional information
generation of a bifunctional enzyme consisting of a GH11 endo-1,4-beta-xylanase fused to a GH43 beta-xylosidase, both from Bacillus subtilis. The substrate cleavage rate is altered by the molecular fusion improving at least 3fold the xylose production using specific substrates as beechwood xylan and hemicelluloses from pretreated biomass. The chimeric enzyme shows higher thermotolerance with a positive shift of the optimum temperature from 35°C to 50°C for xylosidase activity
additional information
fusion of enzyme with a carbohydrate-binding module from Clostridium thermocellum which exhibits high affinity to xylan. The molecular fusion does not alter the pH and temperature dependence, but leads to an increase of 65% in the catalytic efficiency. As supplement in the commercial cocktail Accellerase1 1500, the chimeric enzyme improves the reducing sugar release by 17% from pretreated sugarcane bagasse
additional information
-
fusion of enzyme with a carbohydrate-binding module from Clostridium thermocellum which exhibits high affinity to xylan. The molecular fusion does not alter the pH and temperature dependence, but leads to an increase of 65% in the catalytic efficiency. As supplement in the commercial cocktail Accellerase1 1500, the chimeric enzyme improves the reducing sugar release by 17% from pretreated sugarcane bagasse
additional information
construction of a fusion protein with the carbohydrate-binding doamin of xylanase XynZ from Clostridium thermocellum. The fusion does not alter the pH and temperature dependence, but increases the catalytic activity by 65%
additional information
-
construction of a fusion protein with the carbohydrate-binding doamin of xylanase XynZ from Clostridium thermocellum. The fusion does not alter the pH and temperature dependence, but increases the catalytic activity by 65%
additional information
-
the fusion of the 1642-bp laccase (CorA) with either the 555-bp xylanase (XynA) or the thermostable variant (XynAG3) are performed by insertion of the xylanase into a surface loop of the laccase. The resulting chimeric constructs of 2197 bp contain a central region composed of the XynA or XynAG3 sequence flanked by the regions of the CorA encoding the N-terminal residues 1216 (forming the 5' region of the chimera) and the C-terminal region comprising residues 217513 of CorA. As a consequence, the final construct results in two linkage points between the laccase and xylanase domains
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Kato, Y.; Nevins, D.J.
Enzymic dissociation of Zea shoot cell wall polysaccharides. III. Purification and partial characterization of an endo-(1->4)-beta-D-xylanase
Plant Physiol.
75
753-758
1984
Bacillus subtilis
brenda
Bernier, R.; Desrochers, M.; Jurasek, L.; Paice, M.G.
Isolation and characterization of a xylanase from Bacillus subtilis
Appl. Environ. Microbiol.
46
511-514
1983
Bacillus subtilis, Bacillus subtilis PAP115
brenda
Sa-Pereira, P.; Carvalho, A.S.L.; Costa-Ferreira, M.; Aires-Barros, M.R.
Thermostabilization of Bacillus subtilis CCMI 966 xylanases with trehalose. Study of deactivation kinetics
Enzyme Microb. Technol.
34
278-282
2004
Bacillus subtilis, Bacillus subtilis CCMI 966
-
brenda
Belien, T.; Verjans, P.; Courtin, C.M.; Delcour, J.A.
Phage display based identification of novel stabilizing mutations in glycosyl hydrolase family 11 B. subtilis endoxylanase XynA
Biochem. Biophys. Res. Commun.
368
74-80
2008
Bacillus subtilis
brenda
Dornez, E.; Gebruers, K.; Wiame, S.; Delcour, J.A.; Courtin, C.M.
Insight into the distribution of arabinoxylans, endoxylanases, and endoxylanase inhibitors in industrial wheat roller mill streams
J. Agric. Food Chem.
54
8521-8529
2006
Triticum aestivum, Bacillus subtilis (P18429), Talaromyces purpureogenus (Q9P8J1)
brenda
Verwimp, T.; Van Craeyveld, V.; Courtin, C.M.; Delcour, J.A.
Variability in the structure of rye flour alkali-extractable arabinoxylans
J. Agric. Food Chem.
55
1985-1992
2007
Aspergillus aculeatus, Bacillus subtilis
brenda
Dornez, E.; Cuyvers, S.; Gebruers, K.; Delcour, J.A.; Courtin, C.M.
Contribution of wheat endogenous and wheat kernel associated microbial endoxylanases to changes in the arabinoxylan population during breadmaking
J. Agric. Food Chem.
56
2246-2253
2008
Triticum aestivum, Bacillus subtilis (P18429), Talaromyces purpureogenus (Q9P8J1)
brenda
Bourgois, T.M.; Nguyen, D.V.; Sansen, S.; Rombouts, S.; Belien, T.; Fierens, K.; Raedschelders, G.; Rabijns, A.; Courtin, C.M.; Delcour, J.A.; Van Campenhout, S.; Volckaert, G.
Targeted molecular engineering of a family 11 endoxylanase to decrease its sensitivity towards Triticum aestivum endoxylanase inhibitor types
J. Biotechnol.
130
95-105
2007
Aspergillus niger, Bacillus subtilis (P18429), Bacillus subtilis
brenda
Chiku, K.; Uzawa, J.; Seki, H.; Amachi, S.; Fujii, T.; Shinoyama, H.
Characterization of a novel polyphenol-specific oligoxyloside transfer reaction by a family 11 xylanase from Bacillus sp. KT12
Biosci. Biotechnol. Biochem.
72
2285-2293
2008
Aspergillus niger, Penicillium expansum, Trichoderma viride, Bacillus subtilis (Q45VU2), Bacillus subtilis KT12 (Q45VU2), Penicillium expansum IFO 8800, Aspergillus niger IFO 31628
brenda
Jalal, A.; Rashid, N.; Rasool, N.; Akhtar, M.
Gene cloning and characterization of a xylanase from a newly isolated Bacillus subtilis strain R5
J. Biosci. Bioeng.
107
360-365
2009
Bacillus subtilis (B9ZZN9), Bacillus subtilis R5 (B9ZZN9)
brenda
Lee, J.; Heo, S.; Lee, J.; Yoon, K.; Kim, Y.; Nam, S.
Thermostability and xylan-hydrolyzing property of endoxylanase expressed in yeast Saccharomyces cerevisiae
Biotechnol. Bioprocess Eng.
14
639-644
2009
Bacillus subtilis (Q45VU2)
-
brenda
Rasmussen, L.E.; Soerensen, J.F.; Meyer, A.S.
Kinetics and substrate selectivity of a Triticum aestivum xylanase inhibitor (TAXI) resistant D11F/R122D variant of Bacillus subtilis XynA xylanase
J. Biotechnol.
146
207-214
2010
Bacillus subtilis (P18429)
brenda
Yoon, K.H.
Cloning of a Bacillus subtilis AMX-4 xylanase gene and characterization of the gene product
J. Microbiol. Biotechnol.
19
1514-1519
2009
Bacillus subtilis, Bacillus subtilis AMX-4
brenda
Belien, T.; Joye, I.J.; Delcour, J.A.; Courtin, C.M.
Computational design-based molecular engineering of the glycosyl hydrolase family 11 B. subtilis XynA endoxylanase improves its acid stability
Protein Eng. Des. Sel.
22
587-596
2009
Bacillus subtilis (P18429), Bacillus subtilis
brenda
Ribeiro, L.F.; Furtado, G.P.; Lourenzoni, M.R.; Costa-Filho, A.J.; Santos, C.R.; Nogueira, S.C.; Betini, J.A., Polizeli, Mde L.; Murakami, M.T.; Ward, R.J.
Engineering bifunctional laccase-xylanase chimeras for improved catalytic performance
J. Biol. Chem.
286
43026-4338
2011
Bacillus subtilis
brenda
Diogo, J.A.; Hoffmam, Z.B.; Zanphorlin, L.M.; Cota, J.; Machado, C.B.; Wolf, L.D.; Squina, F.; Damasio, A.R.; Murakami, M.T.; Ruller, R.
Development of a chimeric hemicellulase to enhance the xylose production and thermotolerance
Enzyme Microb. Technol.
69
31-37
2015
Bacillus subtilis (P18429), Bacillus subtilis 168 (P18429)
brenda
Kosciow, K.; Domin, C.; Schweiger, P.; Deppenmeier, U.
Extracellular targeting of an active endoxylanase by a TolB negative mutant of Gluconobacter oxydans
J. Ind. Microbiol. Biotechnol.
43
989-999
2016
Bacillus subtilis (P18429), Bacillus subtilis, Bacillus subtilis 168 (P18429)
brenda
Hoffmam, Z.; Zanphorlin, L.; Cota, J.; Diogo, J.; Almeida, G.; Damasio, A.; Squina, F.; Murakami, M.; Ruller, R.
Xylan-specific carbohydrate-binding module belonging to family 6 enhances the catalytic performance of a GH11 endo-xylanase
New Biotechnol.
33
467-472
2016
Bacillus subtilis (P18429), Bacillus subtilis, Bacillus subtilis 168 (P18429)
brenda
Gagoski, D.; Shi, Z.; Nielsen, L.K.; Vickers, C.E.; Mahler, S.; Speight, R.; Johnston, W.A.; Alexandrov, K.
Cell-free pipeline for discovery of thermotolerant xylanases and endo-1,4-beta-glucanases
J. Biotechnol.
259
191-198
2017
Thermothielavioides terrestris, Bacillus subtilis, Humicola insolens
brenda
Wu, B.; Yu, Q.; Chang, S.; Pedroso, M.M.; Gao, Z.; He, B.; Schenk, G.
Expansin assisted bio-affinity immobilization of endoxylanase from Bacillus subtilis onto corncob residue Characterization and efficient production of xylooligosaccharides
Food Chem.
282
101-108
2019
Bacillus subtilis (A0A172MAU1), Bacillus subtilis
brenda
de Almeida, M.; Guimaraes, V.; Falkoski, D.; de Camargo, B.; Fontes-Santana, G.; Maitan-Alfenas, G.; de Rezende, S.
Purification and characterization of an invertase and a transfructosylase from Aspergillus terreus
J. Food Biochem.
42
e12551
2018
Bacillus subtilis, Bacillus subtilis DFR40
-
brenda